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AB242296

Gamma H2A.X Staining Kit

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(4 Publications)

Gamma H2A.X Staining Kit (ab242296) is based on the phosphorylation of the histone H2A.X at serine 139 in response to DNA damaging agents which cause double strand breaks in cells that are cultured in microtiter plates.

View Alternative Names

H2AFX, H2AX, Histone H2AX, H2a/x, Histone H2A.X

1 Images
Fluorescence Microscopy - Gamma H2A.X Staining Kit (AB242296)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Gamma H2A.X Staining Kit (AB242296)

DNA DSB Formation in A549 Cells.

A549 cells were seeded at 50,000/well overnight, then treated with (B) and without (A) 100 μM Etoposide for 1 hour.

Key facts

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Product details

Gamma H2A.X Staining Kit (ab242296) is based on the phosphorylation of the histone H2A.X at serine 139 in response to DNA damaging agents which cause double strand breaks in cells that are cultured in microtiter plates.

The kit provides sufficient reagents for up to 100 stainings in 96- well plate

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma H2A.X also known as phospho H2A.X or γH2A.X is a phosphorylated form of the histone variant H2A.X. It has a molecular weight of about 14 kilodaltons and occurs primarily in they nucleus. When DNA double-strand breaks (DSBs) occur serine 139 in H2A.X undergoes rapid phosphorylation resulting in gamma H2A.X. This modification happens swiftly at the site of damage and gamma H2A.X spreads over a large chromatin area facilitating the recruitment of DNA repair proteins. Gamma H2A.X staining typically evaluated using gamma H2A.X immunofluorescence techniques aids in identifying the presence and extent of DNA damage.
Biological function summary

Gamma H2A.X plays a role in DNA damage response and repair. It does not operate alone; it acts as part of a complex with other repair proteins. The formation of gamma H2A.X foci at DNA damage sites creates a signal attracting repair factors that help maintain genome stability. Its interaction with MDC1 and ATM proteins exemplifies its significant role in orchestrating an effective response to DNA damage. Beyond DNA repair gamma H2A.X influences cell cycle checkpoints permitting cells to pause and repair before proceeding with division.

Pathways

Gamma H2A.X plays a pivotal role in the DNA damage response (DDR) pathway. This pathway is essential for detecting and repairing DNA lesions to uphold genomic integrity. Within the DDR pathway gamma H2A.X is closely associated with proteins such as NBS1 and BRCA1 which assist in repairing double-strand breaks. In addition gamma H2A.X is integral to the ATM-ATR signaling pathway where its activation promotes cell survival following genotoxic stress by signaling for damage repair or triggering apoptosis.

Gamma H2A.X has connections to cancer and neurodegeneration. Aberrant DNA repair pathways often indicated by persistent gamma H2A.X signals correlate with tumor formation and progression. For instance a failure to repair DNA damage effectively can lead to mutations that drive cancer development. Gamma H2A.X also links to neurodegenerative diseases where dysregulated DNA repair contributes to neuronal cell death. Proteins like p53 which regulate cell cycle and apoptosis further connect to gamma H2A.X bridging its role in disease pathogenesis.
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Product protocols

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Target data

The protein expressed by the H2AX gene is a variant histone H2A that replaces conventional H2A in certain nucleosomes, which are responsible for wrapping and compacting DNA into chromatin. This compaction limits DNA accessibility to cellular machineries that require DNA as a template, placing histones at the center of transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is controlled through a complex array of post-translational histone modifications, known as the histone code, and nucleosome remodeling. The H2AX protein is essential for the checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for the efficient repair of DNA double strand breaks (DSBs), particularly when it undergoes C-terminal phosphorylation. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H2AX
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in aging 5:1466281 PubMed39741583

2025

The aldose reductase inhibitors AT-001, AT-003 and AT-007 attenuate human keratinocyte senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Gautham Yepuri,Kushie Kancharla,Riccardo Perfetti,Shoshana Shendelman,Andrew Wasmuth,Ravichandran Ramasamy

In vivo (Athens, Greece) 38:1546-1556 PubMed38936937

2024

SARS-CoV-2 Spike Protein Induces Oxidative Stress and Senescence in Mouse and Human Lung.

Applications

Unspecified application

Species

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Joel S Greenberger,Wen Hou,Donna Shields,Renee Fisher,Michael W Epperly,Inderpal Sarkaria,Peter Wipf,Hong Wang

ACS applied materials & interfaces 16:4307-4320 PubMed38240181

2024

Safe and Efficacious Near Superhydrophobic Hemostat for Reduced Blood Loss and Easy Detachment in Traumatic Wounds.

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Yibing Dong,Yaoxian Xu,Chengxing Lian,Krisna Prak,Hwa Liang Leo,Teresa D Tetley,Vania Braga,Mike Emerson,Josefin Ahnström,Choon Hwai Yap

Journal of biomedical materials research. Part A 112:770-780 PubMed38095311

2023

Compliant substrates mitigate the senescence associated phenotype of stress induced mesenchymal stromal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Robert C H Gresham,Andrea C Filler,Shierly W Fok,Molly Czachor,Natalie Schmier,Claire Pearson,Chelsea Bahney,J Kent Leach
View all publications
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