Abcam's Activity Assay kits are a set of tools for monitoring cellular functions.
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- Cell death & disease 11:1612020Clarithromycin inhibits autophagy in colorectal cancer by regulating the hERG1 potassium channel interaction with PI3K.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiulia Petroni et. al.PubMed 32123164
- Oncology reports 41:312-3242018Peptides and small molecules blocking the CXCR4/CXCL12 axis overcome bone marrow‑induced chemoresistance in acute leukemias.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSerena Pillozzi,Andrea Bernini,Ottavia Spiga,Barbara Lelli,Giulia Petroni,Luisa Bracci,Neri Niccolai,Annarosa ArcangeliPubMed 30365110
- Molecules (Basel, Switzerland) 23:2018Sinularin Selectively Kills Breast Cancer Cells Showing G2/M Arrest, Apoptosis, and Oxidative DNA Damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHurng-Wern Huang et. al.PubMed 29642488
- Frontiers in physiology 8:6342017Withaferin A Induces Oxidative Stress-Mediated Apoptosis and DNA Damage in Oral Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHsueh-Wei Chang et. al.PubMed 28936177
- Environmental toxicology 32:2124-21322017Sinularin induces oxidative stress-mediated G2/M arrest and apoptosis in oral cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYung-Ting Chang,Chang-Yi Wu,Jen-Yang Tang,Chiung-Yao Huang,Chih-Chuang Liaw,Shih-Hsiung Wu,Jyh-Horng Sheu,Hsueh-Wei ChangPubMed 28548367
- Cancer research 77:3027-30392017E3 Ligase cIAP2 Mediates Downregulation of MRE11 and Radiosensitization in Response to HDAC Inhibition in Bladder Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJudith Nicholson et. al.PubMed 28363998
- Toxins 8:2016Tenuifolide B from Cinnamomum tenuifolium Stem Selectively Inhibits Proliferation of Oral Cancer Cells via Apoptosis, ROS Generation, Mitochondrial Depolarization, and DNA Damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChung-Yi Chen et. al.PubMed 27827950
Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Direct
- Reactive species
- Mammals
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Abcam's Activity Assay kits are a set of tools for monitoring cellular functions.
Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Direct
- Reactive species
- Mammals
- Assay Platform
- Flow cytometer
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Activity Assay kits are a set of tools for monitoring cellular functions. The activation of caspase is widely accepted as a reliable indicator for cell apoptosis. Most caspases have substrate selectivity for the peptide sequence Val-Ala-Asp (VAD). ab112130 uses TF2-VAD-FMK as a fluorescent indicator for most caspase activities. The cell permeable and nontoxic TF2-VAD-FMK irreversibly binds to activated caspase-1, -3, -4, -5, -6, -7, -8 and -9 in apoptotic cells. Once bound to caspases, the fluorescent reagent is retained inside the cell. The binding event prevents the caspases from further catalysis but will not stop apoptosis from proceeding. Within 15 minutes incubation, it starts to react with active caspase enzymes.ab112130 provides all the essential components with an optimized assay protocol. It is designed to detect cell apoptosis by measuring generic activation of caspases (caspase-1, -3, -4, -5, -6, -7, -8 and -9) in live cells.ab112130 is used for either the quantification of most activated caspase activities in apoptotic cells or screening of caspase inhibitors. TF2-VAD-FMK, the green label reagent, allows for direct detection of activated caspases in apoptotic cells by a flow cytometer at Ex/Em = 488/520 nm. Visit our for tips and troubleshooting.
ab112130 should be stored dessicated.**Other caspase and apoptosis assays**Review the full set of , or the .
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Flow Cytometry - Generic Caspase Activity Assay Kit (Fluorometric - Green) (ab112130)
The increase in TF2-VAD-FMK (Component A) fluorescence intensity with the addition of Camptothecin in Jurkat cells.
Jurkat cells were untreated (Blue) or with 20 µM camptothecin (Pink) in a 37 °C, 5 % CO2 incubator for 4-5 hours, and then dye loaded with TF2-VAD-FMK for 1 hour.
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