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GFP Quantification Kit ab235672 quantifies GFP in a 96 microplate format for a wide array of cells and tissues.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

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Images

Functional Studies - GFP Quantification Kit (AB235672), expandable thumbnail

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Key facts

Detection method

Fluorescent

Sample types

Tissue Lysate, Cell culture extracts, Tissue Extracts, Tissue Homogenate, Cell Lysate

Assay type

Quantitative

Range

0.01 - 10 µg/mL

Target data

Function

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Alternative names

Green fluorescent protein, GFP

What's included?

100 Test
Components
GFP Assay Buffer
1 x 25 mL
GFP Standard
1 x 1 Vial
Stop Solution II
1 x 1 mL

Recommended products

GFP Quantification Kit ab235672 quantifies GFP in a 96 microplate format for a wide array of cells and tissues.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Alternative names

Green fluorescent protein, GFP

Key facts

Detection method

Fluorescent

Sample types

Tissue Lysate, Cell culture extracts, Tissue Extracts, Tissue Homogenate, Cell Lysate

Assay type

Quantitative

Range

0.01 - 10 µg/mL

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

The GFP Quantification Kit (ab235672) quantifies GFP in a 96 microplate format for a wide array of cells and tissues.

This kit can detect a wide range of GFP concentrations (0.01-10 μg/ml). Cells or tissues can be homogenized directly in the GFP Assay Buffer, the quantity of GFP is then determined by comparing the fluorescence with that of the GFP standard. Ex/Em = 488/507 nm.

This product is manufactured by BioVision, an Abcam company and was previously called K815 GFP Quantitation Kit. K815-100 is the same size as the 100 test size of ab235672.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Functional Studies - GFP Quantification Kit (ab235672), expandable thumbnail

    Functional Studies - GFP Quantification Kit (ab235672)

    GFP standard curve.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com