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AB235672

GFP Quantification Kit

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(6 Publications)

GFP Quantification Kit ab235672 quantifies GFP in homogenized cell and tissue samples in a mix-and-read, no-wash GFP assay. Readout on fluorometric plate reader.

- Homogenize samples directly in GFP Assay Buffer
- GFP standard included for quantitation

See more GFP assay kits and antibodies

View Alternative Names

Green fluorescent protein, GFP

1 Images
Functional Studies - GFP Quantification Kit (AB235672)
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Supplier Data

Functional Studies - GFP Quantification Kit (AB235672)

GFP standard curve.

Key facts

Detection method

Fluorescent

Sample types

Tissue Lysate, Cell culture extracts, Tissue Extracts, Tissue Homogenate, Cell Lysate

Results type

Quantitative

Range

0.01 - 10 µg/mL

Assay Platform

Microplate reader

Product details

The GFP Quantification Kit (ab235672) quantifies GFP in a 96 microplate format for a wide array of cells and tissues.

This kit can detect a wide range of GFP concentrations (0.01-10 μg/ml). Cells or tissues can be homogenized directly in the GFP Assay Buffer, the quantity of GFP is then determined by comparing the fluorescence with that of the GFP standard. Ex/Em = 488/507 nm.

This product was previously called K815 Biovision GFP Quantitation Kit. Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab235672-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"GFP Standard", "number":"AB235672-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"GFP Assay Buffer", "number":"AB235672-CMP01", "productcode":"" }, { "size":"1 x 1.2 mL", "name":"Stop Solution II", "number":"AB235672-CMP02", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Gut microbes 17:2470386 PubMed39996473

2025

Indole derivatives ameliorated the methamphetamine-induced depression and anxiety via aryl hydrocarbon receptor along "microbiota-brain" axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Wang,Miaoyang Hu,Weilan Wu,Xinyu Lou,Rong Gao,Tengfei Ma,S Thameem Dheen,Jie Cheng,Jianping Xiong,Xufeng Chen,Jun Wang

Biotechnology journal 17:e2100583 PubMed35332662

2022

GFP-tagging of extracellular vesicles for rapid process development.

Applications

Unspecified application

Species

Unspecified reactive species

Braulio Carrillo Sanchez,Matthew Hinchliffe,Daniel G Bracewell

Pharmaceutics 13: PubMed34371766

2021

Oral Delivery of Nucleic Acids with Passive and Active Targeting to the Intestinal Tissue Using Polymer-Based Nanocarriers.

Applications

Unspecified application

Species

Unspecified reactive species

Sagun Poudel,Prabhat R Napit,Karen P Briski,George Mattheolabakis

Thyroid : official journal of the American Thyroid Association 31:202-207 PubMed32718224

2020

Human Type 1 Iodothyronine Deiodinase () Mutations Cause Abnormal Thyroid Hormone Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Monica M França,Alina German,Gustavo W Fernandes,Xiao-Hui Liao,Antonio C Bianco,Samuel Refetoff,Alexandra M Dumitrescu

RSC advances 9:22853-22858 PubMed35514499

2019

Revealing a new fluorescence peak of the enhanced green fluorescent protein using three-dimensional fluorescence spectroscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Nathalia Vieira Dos Santos,Carolina Falaschi Saponi,Tamar Louise Greaves,Jorge Fernando Brandão Pereira

The Plant cell 26:4782-801 PubMed25549672

2014

Soybean miR172c targets the repressive AP2 transcription factor NNC1 to activate ENOD40 expression and regulate nodule initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Youning Wang,Lixiang Wang,Yanmin Zou,Liang Chen,Zhaoming Cai,Senlei Zhang,Fang Zhao,Yinping Tian,Qiong Jiang,Brett J Ferguson,Peter M Gresshoff,Xia Li
View all publications
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Product promise

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