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GFP Quantification Kit ab235672 quantifies GFP in homogenized cell and tissue samples in a mix-and-read, no-wash GFP assay. Readout on fluorometric plate reader.

- Homogenize samples directly in GFP Assay Buffer
- GFP standard included for quantitation

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Functional Studies - GFP Quantification Kit (AB235672), expandable thumbnail

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Key facts

Detection method
Fluorescent
Sample types
Tissue Lysate, Cell culture extracts, Tissue Extracts, Tissue Homogenate, Cell Lysate
Assay type
Quantitative
Range
0.01 - 10 µg/mL

Target data

Function

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Alternative names

Green fluorescent protein, GFP

What's included?

100 Test
Components
GFP Assay Buffer
1 x 25 mL
GFP Standard
1 x 1 Vial
Stop Solution II
1 x 1.2 mL

Recommended products

GFP Quantification Kit ab235672 quantifies GFP in homogenized cell and tissue samples in a mix-and-read, no-wash GFP assay. Readout on fluorometric plate reader.

- Homogenize samples directly in GFP Assay Buffer
- GFP standard included for quantitation

Key facts

Detection method
Fluorescent
Sample types
Tissue Lysate, Cell culture extracts, Tissue Extracts, Tissue Homogenate, Cell Lysate
Assay type
Quantitative
Range
0.01 - 10 µg/mL
Assay Platform
Microplate reader

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

The GFP Quantification Kit (ab235672) quantifies GFP in a 96 microplate format for a wide array of cells and tissues.

This kit can detect a wide range of GFP concentrations (0.01-10 μg/ml). Cells or tissues can be homogenized directly in the GFP Assay Buffer, the quantity of GFP is then determined by comparing the fluorescence with that of the GFP standard. Ex/Em = 488/507 nm.

This product was previously called K815 Biovision GFP Quantitation Kit. Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Functional Studies - GFP Quantification Kit (ab235672), expandable thumbnail

    Functional Studies - GFP Quantification Kit (ab235672)

    GFP standard curve.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com