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AB150670

Giemsa Stain Kit

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(13 Publications)

Giemsa Stain Kit (May-Grunwald) ab150670 is intended for use in the visualization of cells present in hematopoietic tissues and certain microorganisms.
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Histology - Giemsa Stain Kit (AB150670)
  • Histology

Supplier Data

Histology - Giemsa Stain Kit (AB150670)

ab150670 Giemsa Stain Kit staining formalin-fixed-paraffin embedded human bone marrow.

Histology - Giemsa Stain Kit (AB150670)
  • Histology

Supplier Data

Histology - Giemsa Stain Kit (AB150670)

Staining using ab150670 - Giemsa Stain Kit (May-Grunwald).

Histology - Giemsa Stain Kit (AB150670)
  • Histology

Lab

Histology - Giemsa Stain Kit (AB150670)

ab150670 (Giemsa stain) staining mast cells (deep pink) in formalin fixed paraffin embedded normal human colon.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Histology - Giemsa Stain Kit (AB150670)
  • Histology

Supplier Data

Histology - Giemsa Stain Kit (AB150670)

Staining of ab150670 Giemsa Stain Kit.

Key facts

Sample types

Tissue sections

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Product details

Giemsa Stain Kit (May-Grunwald) ab150670 is intended for use in the visualization of cells present in hematopoietic tissues and certain microorganisms. This kit may be used on formalin-fixed, paraffin-embedded or frozen sections.

Giemsa staining protocol summary:
- deparaffinize sections if necessary and rehydrate
- incubate with May-Grunwald solution for 5-7 min
- wash with PBS
- incubate with Giemsa solution for 10-15 min
- wash with PBS, and incubate in PBS for 5 min
- rinse with water and air dry
- clear with Xylene or similar, and mount

Other products for staining tissue sections

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Staining Interpretation

Nuclei Blue/Violet
Cytoplasm Light Blue
Collagen Pale Pink
Muscle Fibre Pale Pink
Erythrocytes Gray, Yellow or Pink
Rickettsia Reddish-Purple
Helicobacter Pylori Blue
Mast Cells Dark Blue with Red Granules

Control Tissue: Blood Film. Any well fixed tissue.

Other Notes

Please note, the 500 mL selling size, was previously described as 100 Test. There has been no change to the physical product.

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What's included?

{ "values": { "500mL": { "sellingSize": "500 mL", "publicAssetCode":"ab150670-500mL", "assetComponentDetails": [ { "size":"1 x 500 mL", "name":"May Grunwald Stock Solution", "number":"AB150670-CMP02" }, { "size":"1 x 500 mL", "name":"Phosphate Buffer solution (pH6.8)", "number":"AB150670-CMP03" }, { "size":"1 x 500 mL", "name":"Giemsa Stock Solution", "number":"AB150670-CMP01" } ] }, "30mL": { "sellingSize": "30 mL", "publicAssetCode":"ab150670-30mL", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"Giemsa Stock Solution", "number":"AB150670-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"May Grunwald Stock Solution", "number":"AB150670-CMP02", "productcode":"" }, { "size":"2 x 60 mL", "name":"Phosphate Buffer solution (pH6.8)", "number":"AB150670-CMP03", "productcode":"" }, { "size":"2 x 1 Each", "name":"Graduated Mixing Vial", "number":"AB150670-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
Storage information
Ambient
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hematopoietic cells are integral components of the blood system responsible for the formation of all blood cells including red and white blood cells and platelets. These cells are primarily found in the bone marrow and express markers such as CD34 contributing to their distinction from other cell types. Microorganisms especially bacteria and parasitic organisms like Plasmodium species interact with these hematopoietic cells during infection processes. Giemsa stain and its variants such as May-Grünwald stain and Giemsa stain kit frequently serve to initially identify and characterize these interactions within blood smears.
Biological function summary

Hematopoietic cells contribute to both regenerative processes and immune responses. They form part of the hematopoietic lineage and associate with several cellular complexes that regulate proliferation differentiation and apoptosis. Bacteria and parasites attach to these cells exploiting them for survival and replication. The use of Giemsa staining as with the May-Grünwald Giemsa method allows scientists to observe these cellular structures and pathogens with clarity. Giemsa stain colors the cells to reveal distinct features useful in pathological diagnostics.

Pathways

Hematopoietic cells engage in pathways such as the JAK-STAT and TLR signaling pathways critical for hematopoiesis and immune modulation. Hematopoietic cells partner with proteins like JAK2 and TLR4 which facilitate the signaling processes essential for cell survival and immune responses. Giemsa staining aids in studying these pathways by enabling the visualization of morphological changes in blood cells as they undergo differentiation or respond to infections.

Hematopoietic cells and their interactions with microorganisms relate closely to conditions such as acute myeloid leukemia and malaria. Mutations in hematopoietic cells can lead to disorders like leukemia involving proteins such as FLT3. Malaria a parasitic infection often involves disruptions to hematopoietic cell populations with Plasmodium pathogens visible under Giemsa staining. Understanding these pathways and interactions through the use of stains supports the diagnosis and treatment of such diseases.
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Product protocols

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Target data

See full target information Giemsa stain, certified by the Biological Stain Commission
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 14:5680-5696 PubMed39803652

2025

NRP1 overexpression potentially enhances osimertinib resistance in NSCLC via activation of the PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Wang,Bao-Lan Wang,Li-Qun Zhou,Yu-Feng Wan,Yu-Long Zheng,Li-Yang Zhou,Ran Fu,Chun-Hua Ling

Oncology letters 28:604 PubMed39483968

2024

infection downregulates hypoxia‑inducible factor 1α expression to suppress the vascularization and tumorigenesis of liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Runling Wu,Xiao Chen,Huan Chen,Mei Li,Yun Liang

Nature communications 15:8914 PubMed39414787

2024

Sensory neurons regulate stimulus-dependent humoral immunity in mouse models of bacterial infection and asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Diane Aguilar,Fengli Zhu,Antoine Millet,Nicolas Millet,Patrizia Germano,Joseph Pisegna,Omid Akbari,Taylor A Doherty,Marc Swidergall,Nicholas Jendzjowsky

Cancer cell international 22:228 PubMed35818076

2022

LINC00893 inhibits the progression of prostate cancer through miR-3173-5p/SOCS3/JAK2/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chuigong Yu,Yu Fan,Yu Zhang,Lupeng Liu,Gang Guo

Bioengineered 13:4185-4200 PubMed35114891

2022

Upregulation of exosomal circPLK1 promotes the development of non-small cell lung cancer through the miR-1294/ high mobility group protein A1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chuankui Li,Guowen Wang,Xiaoxiao Ma,Tao Tao,Qicai Li,Yifan Yang,Haiwei Sang,Zuyi Wang

Oncology letters 23:57 PubMed34992689

2022

LINC01207 promotes prostate cancer progression by sponging miR-1182 to upregulate AKT3.

Applications

Unspecified application

Species

Unspecified reactive species

Daming Qin,Cheng Ni,Biyong Tan,Shengfei Huang,Bingqing Deng,Zhihua Huang

Bioengineered 13:12-26 PubMed34967274

2021

Long non-coding RNA DCST1-AS1/hsa-miR-582-5p/HMGB1 axis regulates colorectal cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Long Huang,Gang Dai

Journal of translational medicine 19:353 PubMed34404435

2021

Fibroblast growth factor 11 (FGF11) promotes non-small cell lung cancer (NSCLC) progression by regulating hypoxia signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowei Wu,Minjie Li,Ying Li,Yu Deng,Shun Ke,Fan Li,Yujin Wang,Shuchang Zhou

Bioengineered 12:4111-4122 PubMed34288804

2021

Circular RNA hsa_circ_0033144 (CircBCL11B) regulates oral squamous cell carcinoma progression via the miR-579/LASP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zeng,Mengmeng Guo,Lin Yao,Zhang Deng

Bioengineered 12:3772-3786 PubMed34281459

2021

Long non-coding RNA DDX11-AS1 promotes esophageal carcinoma cell proliferation and migration through regulating the miR-514b-3p/RBX1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Wu,Zhibin Wang,Xuetao Tian,Jianqiang Wang,Yuesong Zhang,Biao Wu
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