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AB233487

Global DNA Hydroxymethylation Assay Kit (5hmc, Colorimetric)

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(4 Publications)

Global DNA Hydroxymethylation Assay Kit (ab233487) is suitable for detecting global DNA hydroxymethylation levels using DNA isolated from any species including mammals, plants, fungi, bacteria, and viruses in a variety of forms including, but not limited to, cultured cells, fresh and frozen tissues, paraffin-embedded tissues, plasma/serum samples, and body fluid samples.
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Functional Studies - Global DNA Hydroxymethylation Assay Kit (5hmc, Colorimetric) (AB233487)
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Supplier Data

Functional Studies - Global DNA Hydroxymethylation Assay Kit (5hmc, Colorimetric) (AB233487)

5-hmC standard curve.

An example of an optimal standard curve generated with 5-hmC standard control.

Functional Studies - Global DNA Hydroxymethylation Assay Kit (5hmc, Colorimetric) (AB233487)
  • FuncS

Supplier Data

Functional Studies - Global DNA Hydroxymethylation Assay Kit (5hmc, Colorimetric) (AB233487)

Quantification of 5-hmC content of DNA from various species.

Accurate quantification of 5-hmC content of various DNA samples from different species with the The Global DNA Hydroxymethylation Assay kit (ab233487). The results are closely correlated with those obtained by MS-LC.

Key facts

Detection method

Colorimetric

Sample types

DNA

Assay Platform

Microplate reader

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Product details

The Global DNA Hydroxymethylation Assay Kit (ab233487) is suitable for detecting global DNA hydroxymethylation levels using DNA isolated from any species including mammals, plants, fungi, bacteria, and viruses in a variety of forms including, but not limited to, cultured cells, fresh and frozen tissues, paraffin-embedded tissues, plasma/serum samples, and body fluid samples.

In this assay, DNA is bound to strip-wells that are specifically treated to have a high DNA affinity. The hydroxymethylated fraction of DNA is detected using a 5-hmC mAb-based detection complex in a one-step manner and then quantified colorimetrically by reading the absorbance in a microplate spectrophotometer.

DNA methylation occurs by the covalent addition of a methyl group at the 5-carbon of the cytosine ring by DNA methyltransferases, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG, whereas in embryonic stem (ES) cells, a substantial amount of 5-mC is also observed in non-CpG contexts. The biological importance of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely.

5-hydroxymethylcytosine (5-hmC), as a sixth DNA base with functions in transcription regulation, has been detected to be abundant in human and mouse brains and embryonic stem (ES) cells. In mammals, it can be generated by the oxidation of 5-mC, a reaction mediated by the ten-eleven translocation (TET) family of 5-mC-hydroxylases.

5-hmC has been demonstrated to be tissue specific, ranging from undetectable levels in cultured cell lines to 0.6% in human brain tissues, and can be as high as 8% of total DNA in some other species. The biological significance of 5-hmC as an important epigenetic modification in phenotype and gene expression has been recently recognized. For example, global decrease in 5-hmC content (DNA hypohydroxymethylation) has been exhibited in nearly all cancers and has been proposed as a molecular marker and therapeutic target in cancer as well.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

See full target information ATP Synthase
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:848 PubMed36195596

2022

Upregulation of CRABP2 by TET1-mediated DNA hydroxymethylation attenuates mitochondrial apoptosis and promotes oxaliplatin resistance in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong Tang,Yahang Liang,Guorui Sun,Qingsi He,Zhenyu Hou,Xingzhi Jiang,Peng Gao,Hui Qu

Clinical epigenetics 14:89 PubMed35842682

2022

Psychostimulant-induced aberrant DNA methylation in an in vitro model of human peripheral blood mononuclear cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kaili Anier,Kelli Somelar,Külli Jaako,Margret Alttoa,Kerli Sikk,Raul Kokassaar,Kai Kisand,Anti Kalda

Nanomaterials (Basel, Switzerland) 11: PubMed34835707

2021

The Mechanosensing and Global DNA Methylation of Human Osteoblasts on MEW Fibers.

Applications

Unspecified application

Species

Unspecified reactive species

Pingping Han,Cedryck Vaquette,Abdalla Abdal-Hay,Sašo Ivanovski

International journal of molecular sciences 22: PubMed33670900

2021

Salivary Outer Membrane Vesicles and DNA Methylation of Small Extracellular Vesicles as Biomarkers for Periodontal Status: A Pilot Study.

Applications

Unspecified application

Species

Unspecified reactive species

Pingping Han,Peter Mark Bartold,Carlos Salomon,Sašo Ivanovski
View all publications
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