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AB233486

Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric)

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(16 Publications)

Global DNA Methylation Assay Kit (5-Methyl Cytosine) ab233486 contains all reagents necessary for the quantification of global DNA methylation. Readout on any colorimetric (450 nm) plate reader.

- Detection limit as low as 0.05% methylated DNA from 100 ng of input DNA
- High specific for 5-Methylcytosine with no cross-reactivity to unmethylated cytosine or hydroxymethylcytosine within the indicated concentration range of the sample DNA
- Universal positive and negative controls are included for detection of DNA methylation in any species from either single-stranded or double-stranded input DNA

View Alternative Names

5 Methycytosine, 5-Me Cytidine, 5-Me Cytosine, 5-Methyl-Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine, 5-MethylCytosine, 5-mC, 5-meC, methyl CpG

2 Images
Functional Studies - Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric) (AB233486)
  • FuncS

Supplier Data

Functional Studies - Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric) (AB233486)

Accurate quantification of 5-mC content of various DNA samples from different species using Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric.

The results are closely correlated with those obtained by HPLC-MS.

Functional Studies - Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric) (AB233486)
  • FuncS

Supplier Data

Functional Studies - Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric) (AB233486)

An example of an optimal standard curve generated with 5-mC standard control

Key facts

Detection method

Colorimetric

Sample types

DNA

Results type

Quantitative

Assay time

2h

Assay Platform

Microplate reader

Product details

How the assay works

In the global DNA methylation assay ab233486, DNA is bound to strip-wells that are specifically treated to have a high DNA affinity. The methylated fraction of DNA is detected using capture and detection antibodies and then quantified colorimetrically by reading the absorbance in a microplate spectrophotometer. The percentage of methylated DNA is proportional to the OD intensity measured.

Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric) protocol summary:
- Bind DNA to assay wells
- Wash wells, then add detection complex solution.
- Wash and add color developer solution.
- Measure absorbance at 450 nm

How other researchers are using

Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric) has been used in a variety of sample type including:
- Human Tumor and control peritoneal tissues 1
- Human abdominal skin samples 2
- Mouse brain tissues 3

References:
1 - Reamon-Buettner S et al. 2024; 2 - Ghaffarinia A et al. 2023; 3 - Spandole-Dinu S et al. 2023.

Related and recommended products

See an alternative DNA Methylation Assay Kit for the quantification of m6A in DNA:
- m6A DNA Methylation Assay Kit (Colorimetric) ab233488

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Product protocols

Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Particle and fibre toxicology 21:3 PubMed38297314

2024

Malignant peritoneal mesotheliomas of rats induced by multiwalled carbon nanotubes and amosite asbestos: transcriptome and epigenetic profiles.

Applications

Unspecified application

Species

Unspecified reactive species

Stella Marie Reamon-Buettner,Susanne Rittinghausen,Annika Klauke,Andreas Hiemisch,Christina Ziemann

International journal of molecular sciences 25: PubMed38255915

2024

Expression, Functional Polymorphism, and Diagnostic Values of MIAT rs2331291 and H19 rs217727 Long Non-Coding RNAs in Cerebral Ischemic Stroke Egyptian Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Tarek K Motawi,Nermin Abdel Hamid Sadik,Olfat G Shaker,Maggy Maged Haider Ghaleb,Eman M Elbaz

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e23378 PubMed38127104

2023

MIR143HG promotes methylation of transcription factor HOXB7 promoter by recruiting methyltransferase DNMT1 to prevent the progression of colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mo Zhu,Ting Xu,Lindong Ji,Baofei Jiang,Kun Wu

Cells 12: PubMed38132145

2023

Unraveling Transcriptome Profile, Epigenetic Dynamics, and Morphological Changes in Psoriasis-like Keratinocytes: "Insights into Similarity with Psoriatic Lesional Epidermis".

Applications

Unspecified application

Species

Unspecified reactive species

Ameneh Ghaffarinia,Szilárd Póliska,Ferhan Ayaydin,Aniko Goblos,Shahram Parvaneh,Máté Manczinger,Fanni Balogh,Lilla Erdei,Zoltán Veréb,Kornélia Szabó,Zsuzsanna Bata-Csörgő,Lajos Kemény

Nature communications 14:8001 PubMed38049411

2023

Nitric oxide controls shoot meristem activity via regulation of DNA methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zeng,Xin'Ai Zhao,Zhe Liang,Inés Hidalgo,Michael Gebert,Pengfei Fan,Christian Wenzl,Sebastian G Gornik,Jan U Lohmann

PLoS biology 21:e3002033 PubMed36928253

2023

Hypothalamic Menin regulates systemic aging and cognitive decline.

Applications

Unspecified application

Species

Unspecified reactive species

Lige Leng,Ziqi Yuan,Xiao Su,Zhenlei Chen,Shangchen Yang,Meiqin Chen,Kai Zhuang,Hui Lin,Hao Sun,Huifang Li,Maoqiang Xue,Jun Xu,Jingqi Yan,Zhenyi Chen,Tifei Yuan,Jie Zhang

International journal of environmental research and public health 20: PubMed36833719

2023

Pilot Study of the Long-Term Effects of Radiofrequency Electromagnetic Radiation Exposure on the Mouse Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia Spandole-Dinu,Ana-Maria Catrina,Oana Cristina Voinea,Alina Andone,Speranța Radu,Cerasela Haidoiu,Octavian Călborean,Diana Mihaela Popescu,Vladimir Suhăianu,Octavian Baltag,Leontin Tuță,Georgiana Roșu

Cell death & disease 13:848 PubMed36195596

2022

Upregulation of CRABP2 by TET1-mediated DNA hydroxymethylation attenuates mitochondrial apoptosis and promotes oxaliplatin resistance in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong Tang,Yahang Liang,Guorui Sun,Qingsi He,Zhenyu Hou,Xingzhi Jiang,Peng Gao,Hui Qu

Clinical epigenetics 14:89 PubMed35842682

2022

Psychostimulant-induced aberrant DNA methylation in an in vitro model of human peripheral blood mononuclear cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kaili Anier,Kelli Somelar,Külli Jaako,Margret Alttoa,Kerli Sikk,Raul Kokassaar,Kai Kisand,Anti Kalda

Disease markers 2022:7926483 PubMed35860691

2022

miR-26a-5p Suppresses Wnt/-Catenin Signaling Pathway by Inhibiting DNMT3A-Mediated SFRP1 Methylation and Inhibits Cancer Stem Cell-Like Properties of NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yu,Zhe Ge,Shunqiong Chen,Shaoying Li,Xin Zhang,Jie Hu,Wei Guo,Yan Wang
View all publications
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