Global DNA Methylation Assay Kit (5-Methyl Cytosine) ab233486 contains all reagents necessary for the quantification of global DNA methylation. Readout on any colorimetric (450 nm) plate reader.
- Detection limit as low as 0.05% methylated DNA from 100 ng of input DNA
- High specific for 5-Methylcytosine with no cross-reactivity to unmethylated cytosine or hydroxymethylcytosine within the indicated concentration range of the sample DNA
- Universal positive and negative controls are included for detection of DNA methylation in any species from either single-stranded or double-stranded input DNA
Images
Publications
Filter by
- Particle and fibre toxicology 21:32024Malignant peritoneal mesotheliomas of rats induced by multiwalled carbon nanotubes and amosite asbestos: transcriptome and epigenetic profiles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStella Marie Reamon-Buettner,Susanne Rittinghausen,Annika Klauke,Andreas Hiemisch,Christina ZiemannPubMed 38297314
- Cells 12:2023Unraveling Transcriptome Profile, Epigenetic Dynamics, and Morphological Changes in Psoriasis-like Keratinocytes: "Insights into Similarity with Psoriatic Lesional Epidermis".Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmeneh Ghaffarinia,Szilárd Póliska,Ferhan Ayaydin,Aniko Goblos,Shahram Parvaneh,Máté Manczinger,Fanni Balogh,Lilla Erdei,Zoltán Veréb,Kornélia Szabó,Zsuzsanna Bata-Csörgő,Lajos KeményPubMed 38132145
- Nature communications 14:80012023Nitric oxide controls shoot meristem activity via regulation of DNA methylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Zeng,Xin'Ai Zhao,Zhe Liang,Inés Hidalgo,Michael Gebert,Pengfei Fan,Christian Wenzl,Sebastian G Gornik,Jan U LohmannPubMed 38049411
- PLoS biology 21:e30020332023Hypothalamic Menin regulates systemic aging and cognitive decline.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLige Leng,Ziqi Yuan,Xiao Su,Zhenlei Chen,Shangchen Yang,Meiqin Chen,Kai Zhuang,Hui Lin,Hao Sun,Huifang Li,Maoqiang Xue,Jun Xu,Jingqi Yan,Zhenyi Chen,Tifei Yuan,Jie ZhangPubMed 36928253
- International journal of environmental research and public health 20:2023Pilot Study of the Long-Term Effects of Radiofrequency Electromagnetic Radiation Exposure on the Mouse Brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSonia Spandole-Dinu,Ana-Maria Catrina,Oana Cristina Voinea,Alina Andone,Speranța Radu,Cerasela Haidoiu,Octavian Călborean,Diana Mihaela Popescu,Vladimir Suhăianu,Octavian Baltag,Leontin Tuță,Georgiana RoșuPubMed 36833719
- Cell death & disease 13:8482022Upregulation of CRABP2 by TET1-mediated DNA hydroxymethylation attenuates mitochondrial apoptosis and promotes oxaliplatin resistance in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolong Tang,Yahang Liang,Guorui Sun,Qingsi He,Zhenyu Hou,Xingzhi Jiang,Peng Gao,Hui QuPubMed 36195596
- Clinical epigenetics 14:892022Psychostimulant-induced aberrant DNA methylation in an in vitro model of human peripheral blood mononuclear cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaili Anier,Kelli Somelar,Külli Jaako,Margret Alttoa,Kerli Sikk,Raul Kokassaar,Kai Kisand,Anti KaldaPubMed 35842682
- Disease markers 2022:79264832022miR-26a-5p Suppresses Wnt/-Catenin Signaling Pathway by Inhibiting DNMT3A-Mediated SFRP1 Methylation and Inhibits Cancer Stem Cell-Like Properties of NSCLC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Yu,Zhe Ge,Shunqiong Chen,Shaoying Li,Xin Zhang,Jie Hu,Wei Guo,Yan WangPubMed 35860691
- Cells 11:2022Treatment of Pheochromocytoma Cells with Recurrent Cycles of Hypoxia: A New Pseudohypoxic In Vitro Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJana Helm,Stephan Drukewitz,Isabel Poser,Susan Richter,Markus Friedemann,Doreen William,Hermine Mohr,Svenja Nölting,Mercedes Robledo,Stefan R Bornstein,Graeme Eisenhofer,Nicole BechmannPubMed 35159368
- Nanomaterials (Basel, Switzerland) 11:2021The Mechanosensing and Global DNA Methylation of Human Osteoblasts on MEW Fibers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPingping Han,Cedryck Vaquette,Abdalla Abdal-Hay,Sašo IvanovskiPubMed 34835707
Key facts
- Detection method
- Colorimetric
- Sample types
- DNA
- Assay type
- Quantitative
- Assay time
- 2h
Target data
Alternative names
5 Methycytosine, 5-Me Cytidine, 5-Me Cytosine, 5-Methyl-Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine, 5-MethylCytosine, 5-mC, 5-meC, methyl CpG
What's included?
Recommended products
Global DNA Methylation Assay Kit (5-Methyl Cytosine) ab233486 contains all reagents necessary for the quantification of global DNA methylation. Readout on any colorimetric (450 nm) plate reader.
- Detection limit as low as 0.05% methylated DNA from 100 ng of input DNA
- High specific for 5-Methylcytosine with no cross-reactivity to unmethylated cytosine or hydroxymethylcytosine within the indicated concentration range of the sample DNA
- Universal positive and negative controls are included for detection of DNA methylation in any species from either single-stranded or double-stranded input DNA
Key facts
- Detection method
- Colorimetric
- Sample types
- DNA
- Assay type
- Quantitative
- Assay time
- 2h
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
How the assay works
In the global DNA methylation assay ab233486, DNA is bound to strip-wells that are specifically treated to have a high DNA affinity. The methylated fraction of DNA is detected using capture and detection antibodies and then quantified colorimetrically by reading the absorbance in a microplate spectrophotometer. The percentage of methylated DNA is proportional to the OD intensity measured.
Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric) protocol summary:
- Bind DNA to assay wells
- Wash wells, then add detection complex solution.
- Wash and add color developer solution.
- Measure absorbance at 450 nm
How other researchers are using
Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric) has been used in a variety of sample type including:
- Human Tumor and control peritoneal tissues 1
- Human abdominal skin samples 2
- Mouse brain tissues 3
References:
1 - Reamon-Buettner S et al. 2024; 2 - Ghaffarinia A et al. 2023; 3 - Spandole-Dinu S et al. 2023.
Related and recommended products
See an alternative DNA Methylation Assay Kit for the quantification of m6A in DNA:
- m6A DNA Methylation Assay Kit (Colorimetric) m6A DNA Methylation Assay Kit (Colorimetric) ab233488
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric) (ab233486)
Accurate quantification of 5-mC content of various DNA samples from different species using Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric.
The results are closely correlated with those obtained by HPLC-MS.
Functional Studies - Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric) (ab233486)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com