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AB115131

Global Protein Sumoylation Assay Kit

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(2 Publications)

Sumoylation is a post-transcriptional modification involved in various cellular processes such as nuclear-cytoplasmic transport, transcription regulation and progression through the cell cycle.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Assay type

Quantitative

Assay time

5h

Assay Platform

Microplate reader

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Reactivity data

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Product details

Sumoylation is a post-transcriptional modification involved in various cellular processes such as nuclear-cytoplasmic transport, transcription regulation and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2/-3 show a high degree of similarity to each other and are distinct from SUMO-1. Sumoylation is directed by an enzymatic cascade analogous to that involved in ubiquitination. Sumoylation of target proteins in vivo has been shown to cause a number of different outcomes, including altered localization and binding partners. Thus, detection of in vivo protein sumoylation (SUMO conjugation) would provide useful information for understanding SUMO modification as a regulatory control mechanisms for many nuclear proteins.

Global Protein Sumoylation Assay Kit (ab115131) allows the user to measure in vivo protein sumoylation. The kit is ready-to-use and provides all the essential components needed for measuring in vivo protein sumoylation from multiple mammalian cells/tissues extracts.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 10:250 PubMed30867409

2019

SUMOylation of G9a regulates its function as an activator of myoblast proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Shruti Srinivasan,Shilpa Rani Shankar,Yaju Wang,Reshma Taneja

Molecular neurobiology 56:4820-4837 PubMed30402708

2018

Prenatal Exposure to Benzophenone-3 Impairs Autophagy, Disrupts RXRs/PPARγ Signaling, and Alters Epigenetic and Post-Translational Statuses in Brain Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Agnieszka Wnuk,Joanna Rzemieniec,Jakub Staroń,Ewa Litwa,Władysław Lasoń,Andrzej Bojarski,Małgorzata Kajta
View all publications
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