Global Protein Synthesis Assay Kit (ab273286) uses a novel chemical method based on alkyne analog of puromycin, O-Propargyl-puromycin (OP-puro).
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Images
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Assay type
Quantitative
What's included?
Recommended products
Global Protein Synthesis Assay Kit (ab273286) uses a novel chemical method based on alkyne analog of puromycin, O-Propargyl-puromycin (OP-puro).
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Assay type
Quantitative
- Assay Platform
Microplate
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Global Protein Synthesis Assay Kit (ab273286) utilizes a novel chemical method based on alkyne analog of puromycin, O-Propargyl-puromycin (OP-puro). OP-puro stops translation by forming covalent conjugates with the nascent polypeptide chains. Truncated polypeptides are rapidly turned over by the proteasome and can be detected based on a click reaction with the fluorescent azide (Ex/Em = 494/521 nm). OP-puro does not require methionine-free conditions and can be used to label nascent proteins directly in the cell culture medium.
This kit provides sufficient materials for 100 simple and specific assays to detect nascent proteins synthesized under various physiological conditions in real-time, and in the presence of Cycloheximide, an inhibitor of protein synthesis that serves as a control.
This product is manufactured by BioVision, an Abcam company and was previously called K467 EZClick™ Global Protein Synthesis Assay Kit. K467-100 is the same size as the 100 test size of ab273286.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Fluorescence Microscopy - Global Protein Synthesis Assay Kit (ab273286)
Metabolic labeling of protein on proliferating HeLa cells. 1 x 105 cells incubated overnight with fresh aliquots of media containing Protein Label: Upper panel corresponds green fluorescence of de novo synthesized peptides. The lower panel shows panel cells treated with 1X Cycloheximide. Nuclear staining in both panels confirms that green signal is a result of Protein Label incorporation.
Functional Studies - Global Protein Synthesis Assay Kit (ab273286)
Plate reader analyses on Jurkat cells.
Jurkat cells: plate reader analyses of controls and Cycloheximide treatment; Avg fluorescence +/- standard deviation plotted for 3 replicates per condition.
Functional Studies - Global Protein Synthesis Assay Kit (ab273286)
Azide Fluorescence Curve in 0-0.1 X range.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
This is reference data and it should not be used to interpret actual results. Your data will depend on the cell type and tested compound.
Functional Studies - Global Protein Synthesis Assay Kit (ab273286)
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Fluorescence azide curve of Jurkat cells prepared for this assay. Detection limit corresponds to about 31,250 of Jurkat cells per well.
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Product protocols
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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