Glucose-6-Phosphate Assay Kit (Colorimetric) ab83426 is a simple, sensitive and rapid test to quantify glucose-6-phospate (G6P) in a variety of samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Cell reports 41:1115982022MsrB1-regulated GAPDH oxidation plays programmatic roles in shaping metabolic and inflammatory signatures during macrophage activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun Jung Yoo,Dong Wook Choi,Yeon Jin Roh,Yoon-Mi Lee,Ji-Hong Lim,Soohak Eo,Ho-Jae Lee,Na Young Kim,Seohyun Kim,Sumin Cho,Gyumin Im,Byung Cheon Lee,Ji Hyung KimPubMed 36351405
- The New phytologist 235:2331-23492022Two AT-Hook proteins regulate A/NINV7 expression to modulate sucrose catabolism for cold tolerance in Poncirus trifoliata.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBachar Dahro,Yue Wang,Madiha Khan,Yang Zhang,Tian Fang,Ruhong Ming,Chunlong Li,Ji-Hong LiuPubMed 35695205
- Poultry science 101:1016492022Comparison of muscle fiber characteristics and glycolytic potential between slow- and fast-growing broilers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiran Huo,Kaiqi Weng,Yi Li,Yu Zhang,Yang Zhang,Qi Xu,Guohong ChenPubMed 34986445
- BMC plant biology 21:5592021Genome-wide identification and expression profiling of invertase gene family for abiotic stresses tolerance in Poncirus trifoliata.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBachar Dahro,Yue Wang,Ahmed Alhag,Chunlong Li,Dayong Guo,Ji-Hong LiuPubMed 34823468
- Cellular and molecular life sciences : CMLS 78:2857-28762020Low-dose 2-deoxy glucose stabilises tolerogenic dendritic cells and generates potent in vivo immunosuppressive effects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM Christofi,S Le Sommer,C Mölzer,I P Klaska,L Kuffova,J V ForresterPubMed 33074350
- The Journal of cell biology 219:2020The stress-responsive gene GDPGP1/mcp-1 regulates neuronal glycogen metabolism and survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander Schulz,Yuichi Sekine,Motunrayo J Oyeyemi,Alexander J Abrams,Manasa Basavaraju,Sung Min Han,Marco Groth,Helen Morrison,Stephen M Strittmatter,Marc HammarlundPubMed 31968056
- Frontiers in pharmacology 9:10542018Effects of Valproic Acid on Cerebral Nutrient Carriers' Expression in the Rat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAniv Mann Brukner et. al.PubMed 30298005
- Molecular therapy : the journal of the American So 26:1771-17822018Inhibition of Glycogen Synthase II with RNAi Prevents Liver Injury in Mouse Models of Glycogen Storage Diseases.Applications:FuncSReactive species:Unspecified reactive speciesNatalie Pursell et. al.PubMed 29784585
- Medical molecular morphology 51:89-952018H-ATPase blockade reduced renal gluconeogenesis and plasma glucose in a diabetic rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkihiro Tojo et. al.PubMed 29318388
- Cell death discovery 3:161072017IAPP modulates cellular autophagy, apoptosis, and extracellular matrix metabolism in human intervertebral disc cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinghuo Wu,Yu Song,Wei Liu,Kun Wang,Yong Gao,Shuai Li,Zhenfeng Duan,Zengwu Shao,Shuhua Yang,Cao YangPubMed 28149534
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Range
1 - 30 µM
- Assay time
40m
- Sensitivity
= 10 µM
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Glucose-6-Phosphate Assay Kit (Colorimetric) ab83426 is a simple, sensitive and rapid test to quantify glucose-6-phospate (G6P) in a variety of samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Range
1 - 30 µM
- Assay time
40m
- Assay Platform
Microplate reader
- Sensitivity
= 10 µM
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Glucose-6-Phosphate Assay Kit (Colorimetric) (ab83426) is a simple, sensitive and rapid test to quantify glucose-6-phospate (G6P) in a variety of samples. In the assay, glucose-6-phosphate is oxidized into an intermediate product which in turn converts the probe into an intensely colored product with an absorbance at OD=450 nm. This product can detect G6P in the range of 1-30 nmoles with a detection sensitivity ~10 μM G6P.
For higher sensitivity, we recommend our fluorometric assay Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric) (ab107923).
Visit our FAQs page for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called K657 Glucose-6-Phosphate Colorimetric Assay Kit. K657-100 is the same size as the 100 test size of ab83426.
Glucose-6-phosphate (G6P) is a key sugar intermediate for glucose to get into cells, and then enter either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is transformed by G6PDH to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glucose 6-phosphate also known as G6P is an essential intermediary metabolite in cellular functions. It serves as a pivotal molecule in various metabolic pathways. Glucose 6-phosphate forms during the first step of glycolysis through the phosphorylation of glucose by the enzyme hexokinase a reaction that adds a phosphate group. The molecular mass of glucose 6-phosphate is approximately 260.14 g/mol. It is expressed in most cell types throughout the body playing a central role in energy metabolism.
- Biological function summary
Glucose 6-phosphate acts as a substrate for several biochemical processes within the cell. It is an important component in glycogen synthesis and the pentose phosphate pathway providing important intermediates for nucleic acid and lipid synthesis. Although it is not part of a complex in itself glucose 6-phosphate interacts with various enzymes to activate these pathways reflecting its functional significance.
- Pathways
Glucose 6-phosphate is a critical connector in glycolysis and gluconeogenesis. In glycolysis the molecule continues through a series of enzymatic reactions to generate ATP which cells use for energy. Meanwhile in gluconeogenesis glucose 6-phosphate can be converted back to glucose. G6P acts in coordination with enzymes such as glucose-6-phosphate dehydrogenase in the pentose phosphate pathway highlighting its multifunctional nature and its interaction with other proteins like phosphoglucomutase.
- Associated diseases and disorders
Glucose 6-phosphate has significant implications in diabetes and glycogen storage diseases. Diabetes characterized by impaired glucose regulation involves altered levels of glucose 6-phosphate. Aberrant function of glucose 6-phosphatase an enzyme that converts glucose 6-phosphate to glucose can lead to glycogen storage diseases like von Gierke's disease. These disorders connect with glucose 6-phosphate through the disrupted metabolic process underlining its critical role in maintaining normal glucose homeostasis.
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2 product images
Image from Taylor KM et al., PLoS One. 2013;8(2):e56181. Fig 6(A).; doi: 10.1371/journal.pone.0056181. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - Glucose-6-Phosphate Assay Kit (Colorimetric) (ab83426)
G6P (A) levels are elevated in GAA−/− mice compared to wild type (C57/Bl6) and reduced by rhGAA treatment.
GAA−/− mice were dosed with rhGAA. G6P was quantified in heart and triceps homogenates from wild type (C57Bl/6) and GAA−/− mice using ab83426. Values are means ± SEM. Data was analyzed by one-way ANOVA followed by Newman-Keuls comparing groups. ***P<0.001.
Functional Studies - Glucose-6-Phosphate Assay Kit (Colorimetric) (ab83426)
Colorimetric standard curve: mean of duplicates (+/- SD) with background reads subtracted.
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