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AB303735

Glucose-6-Phosphate Dehydrogenase Activity Assay Kit

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The Glucose-6-Phosphate Dehydrogenase Assay kit provides a quick and easy method for monitoring G6PDH activity in a wide variety of samples.
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Functional Studies - Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (AB303735)
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Supplier Data

Functional Studies - Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (AB303735)

Glucose-6-Phosphate Dehydrogenase Activity Assay reaction pathway

Functional Studies - Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (AB303735)
  • FuncS

Supplier Data

Functional Studies - Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (AB303735)

G6PDH specific activity was calculated in lysates prepared from MCf-7 (0.29 µg), MDA-MB-468 (0.41 µg), rat liver (0.6 µg) and orchid leaf (0.56 µg).

Functional Studies - Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (AB303735)
  • FuncS

Supplier Data

Functional Studies - Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (AB303735)

Kinetic measurement of G6PDH activity in various samples.

Functional Studies - Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (AB303735)
  • FuncS

Supplier Data

Functional Studies - Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (AB303735)

NADPH standard curve.

Key facts

Detection method

Fluorescent

Sample types

Cell Lysate, Tissue Lysate

Results type

Quantitative

Sensitivity

~ 1 µU

Assay time

60m

Assay Platform

Microplate reader

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

AB231828

Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)

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Product details

This product is manufactured by BioVision, an Abcam company and was previously sold under product code K751.

The Glucose-6-Phosphate Dehydrogenase Assay kit provides a quick and easy method for monitoring G6PDH activity in a wide variety of samples. In this assay, G6PDH converts G6P into pyruvate and NADPH, which further reduces PicoProbe™ to generate an intense fluorescence product (Ex/Em = 535/587 nm). This kit is simple, sensitive and high-throughput adaptable and can detect as low as 1 µU of G6PDH activity.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucose 6 Phosphate Dehydrogenase also known as G6PD or G6P dehydrogenase plays an important role in the pentose phosphate pathway catalyzing the conversion of glucose 6-phosphate to 6-phosphoglucono-δ-lactone while producing NADPH from NADP+. G6PD with a molecular mass of approximately 59 kDa is expressed in various tissues with high levels found in red blood cells liver and adrenal glands. This enzyme is vital for protecting cells from oxidative damage by supplying reductive capacity.
Biological function summary

Glucose 6-phosphate dehydrogenase is essential for maintaining cellular redox balance especially in cells lacking mitochondria like red blood cells. It functions as part of a monomer which can dimerize depending on the cellular needs and conditions. The activity of G6PD directly affects the production of NADPH which is necessary for the biosynthesis of nucleic acids and lipids and for maintaining reduced glutathione levels therefore sustaining the antioxidant capacity of the cell.

Pathways

Glucose 6-phosphate dehydrogenase is a pivotal enzyme in the oxidative phase of the pentose phosphate pathway which provides ribose 5-phosphate for nucleotide synthesis and NADPH for reductive biosynthetic reactions. G6PD connects with other enzymes in the pathway such as 6-phosphogluconate dehydrogenase contributing to the regulation of cellular metabolic needs. Its function is tightly linked to glucose metabolism and indirectly influences glycolytic processes.

Alterations in glucose 6-phosphate dehydrogenase activity lead to conditions such as G6PD deficiency which can cause hemolytic anemia when individuals are exposed to certain drugs infections or foods. This enzyme's deficiency is one of the most common enzymatic disorders in humans and is triggered by genetic mutations affecting G6PD function. Moreover G6PD has implications in cancer biology as its activity influences the proliferation and survival of cancer cells alongside other proteins like TP53 and NF-κB which are involved in cellular stress responses.
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

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