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AB102529

Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric)

4

(1 Review)

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(31 Publications)

Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (ab102529) is a simple, sensitive and rapid assay detects the activity of Glucose 6 Phosphate Dehydrogenase (G6PDH or G6PD) in a variety of samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

Glucose-6-phosphate 1-dehydrogenase, G6PD, Glucose-6-phosphate dehydrogenase (NAD(P)(+)), zwf

9 Images
Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)
  • FuncS

Lab

Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)

G6PDH Activity measured in cell lysates.

Samples with the concentration of 4e6 cells/mL were used. Samples were undiluted.

Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)
  • FuncS

Lab

Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)

G6PDH Activity measured in mouse tissue lysates. Protein concentration for samples varied from 7 mg/mL to 17 mg/mL. Samples were undiluted.

Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)
  • FuncS

Lab

Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)

G6PDH Activity measured in biological fluids. Samples undiluted.

Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)
  • FuncS

Lab

Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)

G6PDH Activity measured in mouse tissue lysates. Protein concentration for samples varied from 7 mg/mL to 17 mg/mL. Samples were diluted 1-3 fold.

Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)
  • FuncS

Lab

Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)

G6PDH Activity measured in biological fluids. Samples were undiluted.

Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)
  • FuncS

Supplier Data

Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)

Example of Standard curve obtained using ab102529

Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)
  • FuncS

Supplier Data

Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)

Example of positive control and pork liver samples test curves obtained using ab102529

Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)
  • FuncS

Lab

Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)

G6PDH Activity measured in cell lysates.

Samples with the concentration of 4e6 cells/mL were used. Samples were undiluted.

Schematic Diagram - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)

Representative image of Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) ab102529

Components shown from left to right :

- NADH Standard I

- Developer Solution III

- Developer Mix G

- G6PDH Substrate

- Assay Buffer 5

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Assay type

Enzyme activity

Sensitivity

> 0.04 mU/well

Assay time

1h

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (ab102529) is a simple, sensitive and rapid assay detects the activity of Glucose 6 Phosphate Dehydrogenase (G6PDH or G6PD) in a variety of samples.

In the G6PD assay protocol, glucose 6 phosphate is oxidized by G6PD, and NADP is converted to NADPH. This leads to the chemically coupled conversion of a nearly colorless probe to an intensely colored product with an absorbance at 450nm.

The G6PD assay kit can detect as low as 0.04mU G6PD per well.

G6PD assay protocol summary:
- add samples and positive control to wells
- add reaction mix
- analyze on a microplate reader for 5-30 min

This product was previously called K757 Biovision Glucose-6-Phosphate Dehydrogenase Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab102529-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Developer Mix G", "number":"AB102529-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NADH Standard I", "number":"AB102529-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 5", "number":"AB102529-CMP01", "productcode":"" }, { "size":"1 x 1 Each", "name":"Developer Solution III", "number":"AB102529-CMP02", "productcode":"" }, { "size":"1 x 1 Each", "name":"G6PDH Substrate", "number":"AB102529-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucose 6 Phosphate Dehydrogenase also known as G6PD or G6P dehydrogenase plays an important role in the pentose phosphate pathway catalyzing the conversion of glucose 6-phosphate to 6-phosphoglucono-Δ-lactone while producing NADPH from NADP+. G6PD with a molecular mass of approximately 59 kDa is expressed in various tissues with high levels found in red blood cells liver and adrenal glands. This enzyme is vital for protecting cells from oxidative damage by supplying reductive capacity.
Biological function summary

Glucose 6-phosphate dehydrogenase is essential for maintaining cellular redox balance especially in cells lacking mitochondria like red blood cells. It functions as part of a monomer which can dimerize depending on the cellular needs and conditions. The activity of G6PD directly affects the production of NADPH which is necessary for the biosynthesis of nucleic acids and lipids and for maintaining reduced glutathione levels therefore sustaining the antioxidant capacity of the cell.

Pathways

Glucose 6-phosphate dehydrogenase is a pivotal enzyme in the oxidative phase of the pentose phosphate pathway which provides ribose 5-phosphate for nucleotide synthesis and NADPH for reductive biosynthetic reactions. G6PD connects with other enzymes in the pathway such as 6-phosphogluconate dehydrogenase contributing to the regulation of cellular metabolic needs. Its function is tightly linked to glucose metabolism and indirectly influences glycolytic processes.

Alterations in glucose 6-phosphate dehydrogenase activity lead to conditions such as G6PD deficiency which can cause hemolytic anemia when individuals are exposed to certain drugs infections or foods. This enzyme's deficiency is one of the most common enzymatic disorders in humans and is triggered by genetic mutations affecting G6PD function. Moreover G6PD has implications in cancer biology as its activity influences the proliferation and survival of cancer cells alongside other proteins like TP53 and NF-κB which are involved in cellular stress responses.
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Product protocols

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Target data

Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone. Can utilize either NADP(+) or NAD(+).
See full target information Glucose-6-phosphate 1-dehydrogenase
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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Life metabolism 4:loae040 PubMed39872984

2025

Glucose-6-phosphate dehydrogenase regulates mitophagy by maintaining PINK1 stability.

Applications

Unspecified application

Species

Unspecified reactive species

Yik-Lam Cho,Hayden Weng Siong Tan,Jicheng Yang,Basil Zheng Mian Kuah,Nicole Si Ying Lim,Naiyang Fu,Boon-Huat Bay,Shuo-Chien Ling,Han-Ming Shen

Cell death discovery 10:342 PubMed39075050

2024

Deubiquitinase USP9x regulates the proline biosynthesis pathway in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tina Becirovic,Boxi Zhang,Cecilia Lindskog,Erik Norberg,Helin Vakifahmetoglu-Norberg,Vitaliy O Kaminskyy,Elena Kochetkova

International journal of molecular sciences 25: PubMed38928331

2024

The Effect of Neuronal CoQ Deficiency and Mitochondrial Dysfunction on a Rotenone-Induced Neuronal Cell Model of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren Millichap,Nadia Turton,Elisabetta Damiani,Fabio Marcheggiani,Patrick Orlando,Sonia Silvestri,Luca Tiano,Iain P Hargreaves

European heart journal open 4:oeae034 PubMed38854954

2024

Gene expression and ultra-structural evidence for metabolic derangement in the primary mitral regurgitation heart.

Applications

Unspecified application

Species

Unspecified reactive species

Mariame Selma Kane,Juan Xavier Masjoan Juncos,Shajer Manzoor,Maximiliano Grenett,Joo-Yeun Oh,Betty Pat,Mustafa I Ahmed,Clifton Lewis,James E Davies,Thomas S Denney,Jonathan McConathy,Louis J Dell'Italia

International journal of biological sciences 20:1125-1141 PubMed38385081

2024

Diallyl disulfide induces DNA damage and growth inhibition in colorectal cancer cells by promoting POU2F1 ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Longzheng Xia,Jinguan Lin,Mingjing Peng,Xianjie Jiang,Qiu Peng,Shiwen Cui,Wenlong Zhang,Shizhen Li,Jiewen Wang,Linda Oyang,Shiming Tan,Zifan Hu,Nayiyuan Wu,Yanyan Tang,Xia Luo,Zongyao Ren,Yingrui Shi,Qianjin Liao,Yujuan Zhou

iScience 27:109075 PubMed38361607

2024

Sleep fragmentation induces heart failure in a hypertrophic cardiomyopathy mouse model by altering redox metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Karthikeyan Bose,Radhika Agrawal,Thiagarajan Sairam,Jessenya Mil,Matthew P Butler,Perundurai S Dhandapany

Redox biology 69:102994 PubMed38128451

2023

Mitochondrial aldehyde dehydrogenase-2 coordinates the hydrogen sulfide - AMPK axis to attenuate high glucose-induced pancreatic β-cell dysfunction by glutathione antioxidant system.

Applications

Unspecified application

Species

Unspecified reactive species

Udayakumar Karunakaran,Suma Elumalai,Seung Min Chung,Kathrin Maedler,Kyu Chang Won,Jun Sung Moon

Pharmaceutics 15: PubMed37765275

2023

The Synergistic Influence of Polyflavonoids from on Diabetes Treatment and Their Modulation of the PI3K/AKT/FOXO1 Signaling Pathways: Molecular Docking Analyses and In Vivo Investigations.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed A Hassan,Ghada M Abd Elmageed,Ibtehal G El-Qazaz,Doaa S El-Sayed,Lamia M El-Samad,Heba M Abdou

The Journal of biological chemistry 299:105186 PubMed37611829

2023

Metabolic reprogramming contributes to radioprotection by protein kinase Cδ.

Applications

Unspecified application

Species

Unspecified reactive species

Angela M Ohm,Trisiani Affandi,Julie A Reisz,M Cecilia Caino,Angelo D'Alessandro,Mary E Reyland

Neuro-oncology advances 5:vdad092 PubMed37600229

2023

Imaging telomerase reverse transcriptase expression in oligodendrogliomas using hyperpolarized δ-[1-C]-gluconolactone.

Applications

Unspecified application

Species

Unspecified reactive species

Georgios Batsios,Celine Taglang,Anne Marie Gillespie,Pavithra Viswanath
View all publications
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