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AB65334

Glucose and Sucrose Assay Kit

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(3 Publications)

Glucose and Sucrose Assay Kit ab65334 provides a convenient means for measuring glucose and sucrose levels from various biological samples.
3 Images
Functional Studies - Glucose and Sucrose Assay Kit (AB65334)
  • FuncS

Supplier Data

Functional Studies - Glucose and Sucrose Assay Kit (AB65334)

Colorimetric standard calibration Curve of Sucrose.

Functional Studies - Glucose and Sucrose Assay Kit (AB65334)
  • FuncS

Supplier Data

Functional Studies - Glucose and Sucrose Assay Kit (AB65334)

Glucose and Sucrose Assay Kit Fluorometric Standard Curve.

Fluorometric Standard Curves of Sucrose

Other - Glucose and Sucrose Assay Kit (AB65334)
  • Other

Supplier Data

Other - Glucose and Sucrose Assay Kit (AB65334)

Representative image of Glucose and Sucrose Assay Kit ab65334

Components shown from left to right :

- OxiRed Probe

- Invertase

- Sucrose Standard

- Developer Mix B

- Assay Buffer 2

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Results type

Quantitative

Assay time

40m

Assay Platform

Microplate reader

Product details

Abcam's Glucose and Sucrose Assay Kit provides a convenient means for measuring glucose and sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). To measure glucose level, glucose oxidase specifically oxidizes free-glucose generating a compound that reacts with the glucose probe to produce resorufin, which can be detected colorimetrically (OD570nm) or fluorometrically (Ex/Em 535/587). To measure sucrose, invertase can be added to the reaction to convert sucrose to free glucose and fructose, so total glucose level can be measured. Then the sucrose level = Total Glucose – Free Glucose.
Visit our FAQs page for tips and troubleshooting.

Glucose (C6H12O6; FW: 180.16) and sucrose (C12H22O11; FW:342.3) are the important fuel sources to generate universal energy molecule ATP. Measurement of glucose or sucrose level can be very important in both research and development process. Sucrose is a disaccharide which can be converted into one glucose and one fructose when adding Invertase.

This product is manufactured by BioVision, an Abcam company and was previously called K616 Glucose and Sucrose Colorimetric/Fluorometric Assay Kit. K616-100 is the same size as the 100 test size of ab65334.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab65334-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Invertase", "number":"AB65334-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix B", "number":"AB65334-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 2", "number":"AB65334-CMP01", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB65334-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"Sucrose Standard", "number":"AB65334-CMP05", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

See full target information D-Glucose

Additional targets

Sucrose

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

ASN neuro 13:17590914211010647 PubMed33906483

2021

Paeonol inhibits the progression of intracerebral haemorrhage by mediating the HOTAIR/UPF1/ACSL4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng-Long Jin,Wen-Ying Gao,Shao-Jun Liao,Tao Yu,Qing Shi,Shang-Zhen Yu,Ye-Feng Cai

Human molecular genetics 22:2273-82 PubMed23418305

2013

Functional analysis of sucrase-isomaltase mutations from chronic lymphocytic leukemia patients.

Applications

Unspecified application

Species

Unspecified reactive species

David Rodríguez,Andrew J Ramsay,Víctor Quesada,Cecilia Garabaya,Elías Campo,José M P Freije,Carlos López-Otín

Proceedings of the National Academy of Sciences of the United States of America 109:90-4 PubMed22198770

2011

Incorporating microorganisms into polymer layers provides bioinspired functional living materials.

Applications

Unspecified application

Species

Unspecified reactive species

Lukas C Gerber,Fabian M Koehler,Robert N Grass,Wendelin J Stark
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