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Functional Studies - Glucose Assay Kit (AB272532)
Glucose Assay Kit standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Product details
Simple, direct and automation-ready procedures for measuring glucose concentrations find wide applications in research and drug discovery. Glucose Assay Kit (ab272532) is designed to measure glucose directly in serum or plasma without any pretreatment. The improved otoluidine method utilizes a specific color reaction with glucose. The absorbance at 630nm is directly proportional to glucose concentration in the sample.
Sensitive and accurate: Use as little as 5 μL samples. Linear detection range 0.7 mg/dL (39 μM) to 300 mg/dL (16.6 mM) glucose in 96-well plate.
Simple and convenient: The procedure involves addition of a single working reagent and incubation for 8 min in a boiling water bath. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.
Low interference in biological samples: No pretreatments are needed. Assays can be directly performed on serum and plasma samples.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information
Shipped at conditions
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Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Glucose serves as the primary energy substrate for cells providing energy through glycolysis and oxidative phosphorylation. It is not part of any protein complexes but it interacts with numerous enzymes and proteins to regulate metabolic processes. Glucose operates in maintaining homeostasis and the brain relies on it almost exclusively for energy. Glucose assay reagents and glucose detection kits are utilized to quantify glucose concentrations in research studies examining these functions.
Pathways
Glucose is a central component in glycolysis and the tricarboxylic acid (TCA) cycle. In glycolysis glucose is broken down into pyruvate generating ATP and NADH in the process. This pathway involves key regulatory proteins such as hexokinase and phosphofructokinase. In the TCA cycle glucose metabolites further produce ATP and CO2 involving enzymes like citrate synthase. Glucose uptake assays provide insights into how these pathways operate under various physiological conditions.
Product protocols
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Publications (6)
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Nature communications 15:6587 PubMed39097623
2024
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Nature communications 15:6046 PubMed39025848
2024
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Nature communications 14:7674 PubMed37996418
2023
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Bone 170:116672 PubMed36646266
2023
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Physiological reports 10:e15357 PubMed35851836
2022
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RSC advances 11:39582-39592 PubMed35492494
2021
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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