Glucose Assay Kit

• FuncS

Lab

Functional Studies - Glucose Assay Kit (AB65333)

Glucose measured in cell lysates showing quantity (nmol) per million cells.

Samples with the concentration of 2x10⁷ cells/mL were used. Samples were diluted 1.5-13.5 fold and measured colorimetrically.

• Biochemical assay

Lab

Biochemical assay - Glucose Assay Kit (AB65333)

Diagram showing the principles of the Glucose assay method.

• FuncS

Lab

Functional Studies - Glucose Assay Kit (AB65333)

Glucose measured in human biological fluids showing quantity (μmol) per mL of tested sample. Samples were diluted 13.5 fold and measured colorimetrically.

• FuncS

PubMed

Functional Studies - Glucose Assay Kit (AB65333)

Shao et al investigated the functional outcome of long- term curcumin supplementation on glucose homeostasis. Glucose metabolism was determined in animals with low fat diet (LFD), high fat diet (HFD) and HFD with curcumin feeding using ab65333. Intraperitoneal insulin tolerance tests(IPITT) were conducted at the end of the 26 weeks. It was concluded curcumin improves insulin sensitivity and disposal of glucose.

Image from Shao W et al., PLoS One 7(1), Fig 2C. Doi: 10.1371/journal.pone.0028784. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• FuncS

Lab

Functional Studies - Glucose Assay Kit (AB65333)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

• FuncS

Lab

Functional Studies - Glucose Assay Kit (AB65333)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

• Biochemical assay

PubMed

Biochemical assay - Glucose Assay Kit (AB65333)

Glucose colorimetric assay kit ab65333 used for glucose quantitation in conditioned cell culture media from human cerebral organoids.

Fertan et al established the glucose colorimetric assay kit as a normalization tool for the number of living cells in various cerebral organoid cultures. Image compares remaining glucose levels in cell culture media between low density samples (2 organoids) and high density samples (8-11 organoids). Fertan et al then used normalization using the glucose assay kit as part of measuring small, soluble aggregates of beta-amyloid and tau produced by cerebral organoids in Alzheimer’s disease research.

• Biochemical assay

PubMed

Biochemical assay - Glucose Assay Kit (AB65333)

Glucose colorimetric assay kit ab65333 used to measure glucose in human hepatocellular carcinoma tissue.

Zhou at al measure relative glucose level in 30 paired hepatocellular carcinoma and adjacent nontumoral tissues. The results showed that glucose levels were strikingly decreased in HCC tissues compared to those in adjacent nontumoral tissue samples. Tissues were dissolved in double distilled water and homogenized by ultrasonic treatment. A PCA/KOH deproteinization step was performed before glucose assay. All the steps were conducted as the protocol described.

• Biochemical assay

PubMed

Biochemical assay - Glucose Assay Kit (AB65333)

Shao et al used Lactate assay kit ab65330 and Glucose assay kit ab65333 to investigate the impact of Salvigenin, a potential therapeutic) on glycolysis with cell culture supernatant of HepG2 cells.

Salvigenin repressed HCC cell aerobic glycolysis. Salvigenin (25 µM, 50 µM, 100 µM) was utilized to treat HepG2 cells. The level of glucose uptake. The level of lactate production.

The supernatant of the mediums of HepG2 cells was harvested. The floating cells and cell debris were removed through 5 min of centrifugation (1000 g, 4 °C). Then, the supernatant was harvested. As instructed by the manufacturer, the glucose uptake detection kit (ab65333, Abcam, USA) and the lactate production detection kit (ab65330, Abcam, USA) were taken to determine the levels of glucose uptake and lactate generation.

• Biochemical assay

PubMed

Biochemical assay - Glucose Assay Kit (AB65333)

Carreno-Florez et al used Lactate assay kit ab65330 and Glucose assay kit ab65333 to investigate glycolytic flux and the Warburg effect in cultured human ΔF508/ΔF508 cystic fibrosis airway epithelial cells (CFBE41o-, hereafter called CF AECs).

Glucose consumption in the basolateral medium of IFN-β-stimulated or influenza virus and respiratory syncytial virus (RSV)-infected CF AECs measured by colorimetric assay. L-lactate concentration measured in apical secretions from CF AECs during IFN-β stimulation or RSV infection measured by colorimetric assay. For all experiments n ≥ 3. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Glucose consumption (Abcam, ab65333) and lactate secretion (Abcam, ab65330) were measured after 18 h of IFN-β stimulation or 72 h of RSV infection using colorimetric assays. Glucose consumption was determined by subtracting the glucose concentration in basolateral medium from the glucose concentration in the feeding medium. For lactate secretion, MEM without phenol red was added apically for 18 h during IFN-β stimulation or for the last 24 h of RSV infection and was collected to determine the extracellular lactate concentration.

• Other

Supplier Data

Other - Glucose Assay Kit (AB65333)

Representative image of Glucose Assay Kit ab65333

Components shown from left to right :

- OxiRed Probe

- Glucose Standard

- Developer Mix B

- Assay Buffer 2

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.