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Glucose Assay Kit ab65333 is a rapid, simple and sensitive assay used to quantify glucose levels in biological samples such as serum, plasma, and other body fluids, food, growth medium, etc.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

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Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Urine, Plasma, Cell culture supernatant, Serum, Other biological fluids, Tissue Lysate, Cell Lysate

Assay type

Quantitative

Range

1 - 10000 µM

Assay time

40m

Sensitivity

= 1 µM

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What's included?

2000 Test
Components
Assay Buffer II
20 x 25 mL
Development Enzyme Mix II
20 x 1 Vial
Glucose Standard
20 x 100 µL
OxiRed Probe
20 x 200 µL

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Glucose Assay Kit ab65333 is a rapid, simple and sensitive assay used to quantify glucose levels in biological samples such as serum, plasma, and other body fluids, food, growth medium, etc.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Urine, Plasma, Cell culture supernatant, Serum, Other biological fluids, Tissue Lysate, Cell Lysate

Assay type

Quantitative

Range

1 - 10000 µM

Assay time

40m

Assay Platform

Microplate reader

Sensitivity

= 1 µM

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Glucose Assay Kit ab65333 is a rapid, simple and sensitive assay used to quantify glucose levels in biological samples such as serum, plasma, and other body fluids, food, growth medium, etc.

In the glucose assay protocol, the glucose enzyme mix oxidizes glucose to generate a product which reacts with a dye to generate color (λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The generated color and fluorescence is proportionally to the amount of glucose.

The kit detects glucose in the range 1-10000 μM.

Glucose assay protocol summary:
- add samples (deproteinized) and standards to wells
- add reaction mix and incubate for 30 min at 37°C
- analyze with microplate reader

If you have reducing substances in your samples, we recommend using Glucose Detection Kit II (ab102517). Review our to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Functional Studies - Glucose Assay Kit (ab65333), expandable thumbnail
    Image from Shao W et al., PLoS One 7(1), Fig 2C. Doi: 10.1371/journal.pone.0028784. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Functional Studies - Glucose Assay Kit (ab65333)

    Shao et al investigated the functional outcome of long- term curcumin supplementation on glucose homeostasis. Glucose metabolism was determined in animals with low fat diet (LFD), high fat diet (HFD) and HFD with curcumin feeding using ab65333. Intraperitoneal insulin tolerance tests(IPITT) were conducted at the end of the 26 weeks. It was concluded curcumin improves insulin sensitivity and disposal of glucose.

  • Functional Studies - Glucose Assay Kit (ab65333), expandable thumbnail

    Functional Studies - Glucose Assay Kit (ab65333)

    Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Functional Studies - Glucose Assay Kit (ab65333), expandable thumbnail

    Functional Studies - Glucose Assay Kit (ab65333)

    Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Functional Studies - Glucose Assay Kit (ab65333), expandable thumbnail

    Functional Studies - Glucose Assay Kit (ab65333)

    Glucose measured in cell lysates showing quantity (nmol) per million cells.

    Samples with the concentration of 2x107 cells/mL were used. Samples were diluted 1.5-13.5 fold and measured colorimetrically.

  • Functional Studies - Glucose Assay Kit (ab65333), expandable thumbnail

    Functional Studies - Glucose Assay Kit (ab65333)

    Glucose measured in human biological fluids showing quantity (μmol) per mL of tested sample. Samples were diluted 13.5 fold and measured colorimetrically.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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