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AB169559

Glucose Assay Kit (Fluorometric, High Sensitivity)

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(10 Publications)

Glucose Assay Kit (ab169559) is a simple, rapid, ultra-sensitive assay to measure glucose.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - Glucose Assay Kit (Fluorometric, High Sensitivity) (AB169559)
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Supplier Data

Functional Studies - Glucose Assay Kit (Fluorometric, High Sensitivity) (AB169559)

Measurement of glucose levels in samples. Measurement of glucose levels in human serum (1 µL of 1 : 10 diluted) and rat tissue lysates from liver, kidney and muscle (0.14 µg, 0.19 µg and 0.93 µg respectively).

Functional Studies - Glucose Assay Kit (Fluorometric, High Sensitivity) (AB169559)
  • FuncS

Supplier Data

Functional Studies - Glucose Assay Kit (Fluorometric, High Sensitivity) (AB169559)

Glucose standard curve. Representative standard curve.

Key facts

Detection method

Fluorescent

Sample types

Plasma, Tissue, Suspension cells, Serum, Other biological fluids, Adherent cells, Tissue Culture Media

Assay type

Quantitative

Sensitivity

< 0.5 µM

Assay Platform

Microplate reader

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Product details

Glucose Assay Kit (ab169559) is a simple, rapid, ultra-sensitive assay to measure glucose. It is suitable for high-throughput use. In this assay, D-glucose is enzymatically oxidized to form a product which reacts with a colorless probe to generate the fluorescence (Ex/Em = 535/587 nm). The fluorescence generated is directly proportional to the amount of glucose. This assay kit can detect less than 0.5 uM glucose in various biological samples.

Other Notes
This product was previously called K688 Biovision PicoProbe Glucose Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

Glucose is the main energy source for virtually all living organisms. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose can be very important in both research and drug discovery processes.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab169559-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Substrate Mix I", "number":"AB169559-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Glucose Enzyme Mix", "number":"AB169559-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"Glucose Standard", "number":"AB169559-CMP03", "productcode":"" }, { "size":"1 x 0.4 mL", "name":"PicoProbe I", "number":"AB169559-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 2", "number":"AB169559-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucose often referred to as blood sugar is a simple sugar and an essential carbohydrate in biology. It has a molecular mass of 180.16 g/mol and is highly soluble in water. Glucose circulates in the bloodstream and is absorbed by tissues mainly liver muscle and adipose tissue. It serves as a critical energy source and cells use glucose uptake processes to transport glucose across their membranes. Various diagnostic tools and kits such as glucose assay kits and glucose test kits help measure glucose levels in biological samples.
Biological function summary

Glucose serves as the primary energy substrate for cells providing energy through glycolysis and oxidative phosphorylation. It is not part of any protein complexes but it interacts with numerous enzymes and proteins to regulate metabolic processes. Glucose operates in maintaining homeostasis and the brain relies on it almost exclusively for energy. Glucose assay reagents and glucose detection kits are utilized to quantify glucose concentrations in research studies examining these functions.

Pathways

Glucose is a central component in glycolysis and the tricarboxylic acid (TCA) cycle. In glycolysis glucose is broken down into pyruvate generating ATP and NADH in the process. This pathway involves key regulatory proteins such as hexokinase and phosphofructokinase. In the TCA cycle glucose metabolites further produce ATP and CO2 involving enzymes like citrate synthase. Glucose uptake assays provide insights into how these pathways operate under various physiological conditions.

Glucose regulation and metabolism are tightly linked to diabetes mellitus and metabolic syndrome. Diabetes mellitus is characterized by impaired glucose uptake and insulin regulation often involving insulin receptor pathways. Persistent high glucose levels lead to complications such as neuropathy and retinopathy. AMP-activated protein kinase (AMPK) plays an important role in metabolic syndrome by affecting glucose uptake and energy balance. Understanding glucose's role in these diseases is central to devising therapeutic strategies and interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information D-Glucose
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 24:e55643 PubMed36592158

2023

MYB sustains hypoxic survival of pancreatic cancer cells by facilitating metabolic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Shashi Anand,Mohammad Aslam Khan,Haseeb Zubair,Sarabjeet Kour Sudan,Kunwar Somesh Vikramdeo,Sachin Kumar Deshmukh,Shafquat Azim,Sanjeev Kumar Srivastava,Seema Singh,Ajay Pratap Singh

Journal of oncology 2022:6785956 PubMed35957803

2022

MITF-Mediated lncRNA CCDC183-As1 Promotes the Tumorigenic Properties and Aerobic Glycolysis of Bladder Cancer via Upregulating TCF7L2.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Cai,Meng Wei,Zhen Su

Bioengineered 13:2554-2567 PubMed35156520

2022

LncRNA LINC00525 activates HIF-1α through miR-338-3p / UBE2Q1 / β-catenin axis to regulate the Warburg effect in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fanqi Meng,Xiaofan Luo,Chenyao Li,Guangyi Wang

iScience 24:103056 PubMed34755080

2021

Real-time monitoring of deformed wing virus-infected bee foraging behavior following histone deacetylase inhibitor treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Kang Tang,Yu-Hsien Lin,Joe-Air Jiang,Yun-Heng Lu,Chih-Hsuan Tsai,Yu-Chun Lin,Yun-Ru Chen,Carol-P Wu,Yueh-Lung Wu

Frontiers in cell and developmental biology 9:655018 PubMed34513821

2021

LncRNA OIP5-AS1 Regulates the Warburg Effect Through miR-124-5p/IDH2/HIF-1α Pathway in Cervical Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Yan Ma,Kamalibaike Maerkeya,Davuti Reyanguly,Lili Han

Cell metabolism 31:1189-1205.e13 PubMed32433922

2020

GLP-1 Receptor Signaling in Astrocytes Regulates Fatty Acid Oxidation, Mitochondrial Integrity, and Function.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Timper,Almudena Del Río-Martín,Anna Lena Cremer,Stephan Bremser,Jens Alber,Patrick Giavalisco,Luis Varela,Christian Heilinger,Hendrik Nolte,Aleksandra Trifunovic,Tamas L Horvath,Peter Kloppenburg,Heiko Backes,Jens C Brüning

Scientific reports 9:15665 PubMed31666638

2019

UGCG influences glutamine metabolism of breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Schömel,Sarah E Hancock,Lisa Gruber,Ellen M Olzomer,Frances L Byrne,Divya Shah,Kyle L Hoehn,Nigel Turner,Sabine Grösch,Gerd Geisslinger,Marthe-Susanna Wegner

PloS one 13:e0193560 PubMed29518119

2018

Arylamine N-acetyltransferase 1 protects against reactive oxygen species during glucose starvation: Role in the regulation of p53 stability.

Applications

Unspecified application

Species

Unspecified reactive species

LiLi Wang,Rodney F Minchin,Neville J Butcher

Free radical biology & medicine 99:608-622 PubMed27515816

2016

Hormetic shifting of redox environment by pro-oxidative resveratrol protects cells against stress.

Applications

Unspecified application

Species

Unspecified reactive species

Annabell Plauth,Anne Geikowski,Susanne Cichon,Sylvia J Wowro,Linda Liedgens,Morten Rousseau,Christopher Weidner,Luise Fuhr,Magdalena Kliem,Gail Jenkins,Silvina Lotito,Linda J Wainwright,Sascha Sauer

FEBS letters 590:2076-85 PubMed27264935

2016

The tumor suppressor gene lkb1 is essential for glucose homeostasis during zebrafish early development.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Kuang,Chao Liu,Junshun Fang,Weirui Ma,Jian Zhang,Sheng Cui
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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