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AB102517

Glucose Assay Kit - reducing agent compatible

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(43 Publications)

Glucose Assay Kit ab102517 provides a method for measurement of glucose in biological samples; this method is recommended for serum and urine samples as it is not affected by reducing substances.
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Functional Studies - Glucose Assay Kit - reducing agent compatible (AB102517)
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Functional Studies - Glucose Assay Kit - reducing agent compatible (AB102517)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

Functional Studies - Glucose Assay Kit - reducing agent compatible (AB102517)
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Supplier Data

Functional Studies - Glucose Assay Kit - reducing agent compatible (AB102517)

Standard curve for glucose run using the kit protocol

Functional Studies - Glucose Assay Kit - reducing agent compatible (AB102517)
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Functional Studies - Glucose Assay Kit - reducing agent compatible (AB102517)

Glucose measured in biological fluids. Human samples diluted 20-80 fold. Mouse samples diluted 1-27 fold.

Other - Glucose Assay Kit - reducing agent compatible (AB102517)
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Supplier Data

Other - Glucose Assay Kit - reducing agent compatible (AB102517)

Representative image of Glucose Assay Kit - reducing agent compatible ab102517

Components shown from left to right :

- Glucose Standard

- Developer Solution III

- Developer Mix D

- Assay Buffer 34

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Results type

Quantitative

Sensitivity

> 0.02 mM

Range

0.02 - 10 mM

Assay time

30m

Assay Platform

Microplate reader

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Product details

Glucose Assay Kit ab102517 provides direct measurement of glucose in biological samples. It is particularly suitable for serum and urine samples since it is not affected by reducing substances, which can interfere with detection in oxidase-based kits.

Glucose assay principle
In the glucose assay protocol, glucose is acted on by glucose dehydrogenase. The reaction is coupled by NADH to the generation of a colored dye (450 nm) whose intensity is proportional to glucose concentration.

The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes.

The kit can detect glucose concentrations in the range of 20uM-10mM.

Glucose assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with a microplate reader

Other Notes
This product was previously called K686 Biovision Glucose Colorimetric Assay Kit II. Biovision was acquired by Abcam in 2021.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab102517-100Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"Assay Buffer 34", "number":"AB102517-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Glucose Standard", "number":"AB102517-CMP02", "productcode":"" }, { "size":"1 x 1 Each", "name":"Developer Solution III", "number":"AB102517-CMP03", "productcode":"AB255098" }, { "size":"1 x 1 Each", "name":"Developer Mix D", "number":"AB102517-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucose often referred to as blood sugar is a simple sugar and an essential carbohydrate in biology. It has a molecular mass of 180.16 g/mol and is highly soluble in water. Glucose circulates in the bloodstream and is absorbed by tissues mainly liver muscle and adipose tissue. It serves as a critical energy source and cells use glucose uptake processes to transport glucose across their membranes. Various diagnostic tools and kits such as glucose assay kits and glucose test kits help measure glucose levels in biological samples.
Biological function summary

Glucose serves as the primary energy substrate for cells providing energy through glycolysis and oxidative phosphorylation. It is not part of any protein complexes but it interacts with numerous enzymes and proteins to regulate metabolic processes. Glucose operates in maintaining homeostasis and the brain relies on it almost exclusively for energy. Glucose assay reagents and glucose detection kits are utilized to quantify glucose concentrations in research studies examining these functions.

Pathways

Glucose is a central component in glycolysis and the tricarboxylic acid (TCA) cycle. In glycolysis glucose is broken down into pyruvate generating ATP and NADH in the process. This pathway involves key regulatory proteins such as hexokinase and phosphofructokinase. In the TCA cycle glucose metabolites further produce ATP and CO2 involving enzymes like citrate synthase. Glucose uptake assays provide insights into how these pathways operate under various physiological conditions.

Glucose regulation and metabolism are tightly linked to diabetes mellitus and metabolic syndrome. Diabetes mellitus is characterized by impaired glucose uptake and insulin regulation often involving insulin receptor pathways. Persistent high glucose levels lead to complications such as neuropathy and retinopathy. AMP-activated protein kinase (AMPK) plays an important role in metabolic syndrome by affecting glucose uptake and energy balance. Understanding glucose's role in these diseases is central to devising therapeutic strategies and interventions.
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Product protocols

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Target data

See full target information D-Glucose
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Publications (43)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9729 PubMed39523369

2024

Base-editing corrects metabolic abnormalities in a humanized mouse model for glycogen storage disease type-Ia.

Applications

Unspecified application

Species

Unspecified reactive species

Irina Arnaoutova,Yvonne Aratyn-Schaus,Lisa Zhang,Michael S Packer,Hung-Dar Chen,Cheol Lee,Sudeep Gautam,Francine M Gregoire,Dominique Leboeuf,Steven Boule,Thomas P Fernandez,Victoria Huang,Lo-I Cheng,Genesis Lung,Brianna Bannister,Jeremy Decker,Thomas Leete,Lan S Shuang,Caroline Bock,Prachi Kothiyal,Phil Grayson,Ka W Mok,Jeffrey J Quinn,Lauren Young,Luis Barrera,Giuseppe Ciaramella,Brian C Mansfield,Janice Y Chou

iScience 27:110217 PubMed38993663

2024

A neutral invertase controls cell division besides hydrolysis of sucrose for nutrition during germination and seed setting in rice.

Applications

Unspecified application

Species

Unspecified reactive species

Zizhang Wang,Hao Li,Yuxiang Weng

The Journal of biological chemistry 300:105637 PubMed38199564

2024

Circadian modulation of glucose utilization via CRY1-mediated repression of Pdk1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ying Chiou,Cing-Yun Lee,Hao-Wei Yang,Wei-Cheng Cheng,Kun-Da Ji

Frontiers in immunology 14:1196704 PubMed37215106

2023

Epigenetically altered macrophages promote development of diabetes-associated atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Huang,Wei Gao,Xin Zhong,Hongxian Wu,You Zhou,Yuanji Ma,Juying Qian,Junbo Ge

Molecular metabolism 70:101692 PubMed36773648

2023

Osmoadaptive GLP-1R signalling in hypothalamic neurones inhibits antidiuretic hormone synthesis and release.

Applications

Unspecified application

Species

Unspecified reactive species

Michael P Greenwood,Mingkwan Greenwood,Soledad Bárez-López,Joe W Hawkins,Katherine Short,Danijela Tatovic,David Murphy

Biomedicines 10: PubMed35203687

2022

A Novel 2-Hit Zebrafish Model to Study Early Pathogenesis of Non-Alcoholic Fatty Liver Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Abhishek Kulkarni,Sara Ibrahim,Isra Haider,Amina Basha,Emma Montgomery,Ebru Ermis,Raghavendra G Mirmira,Ryan M Anderson

Cell reports 37:110113 PubMed34910917

2021

Streptozotocin-induced hyperglycemia alters the cecal metabolome and exacerbates antibiotic-induced dysbiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jenna I Wurster,Rachel L Peterson,Claire E Brown,Swathi Penumutchu,Douglas V Guzior,Kerri Neugebauer,William H Sano,Manu M Sebastian,Robert A Quinn,Peter Belenky

Frontiers in oncology 11:733595 PubMed34527595

2021

Identification of Glycolysis-Related lncRNAs and the Novel lncRNA WAC-AS1 Promotes Glycolysis and Tumor Progression in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xigang Xia,Hao Zhang,Peng Xia,Yimin Zhu,Jie Liu,Kequan Xu,Yufeng Yuan

Analytical cellular pathology (Amsterdam) 2021:9927720 PubMed34336555

2021

miR-142-3p Modulates Cell Invasion and Migration via PKM2-Mediated Aerobic Glycolysis in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

JunYu Ren,Wenliang Li,Guoqing Pan,Fengchang Huang,Jun Yang,Hongbin Zhang,Ruize Zhou,Ning Xu

Molecular metabolism 53:101289 PubMed34246804

2021

β-Cell pre-mir-21 induces dysfunction and loss of cellular identity by targeting transforming growth factor beta 2 (Tgfb2) and Smad family member 2 (Smad2) mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Ibrahim,Macey Johnson,Clarissa Hernandez Stephens,Jerry Xu,Rachel Moore,Andrea Mariani,Christopher Contreras,Farooq Syed,Raghavendra G Mirmira,Ryan M Anderson,Emily K Sims
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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