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AB219924

Glucose Oxidase Activity Assay Kit (Colorimetric)

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(4 Publications)

Glucose Oxidase Activity Assay Kit ab219924 uses a simple colorimetric assay to measure glucose oxidase (GOx) activity in cell and tissue extracts, and biological fluids.

View Alternative Names

Glucose oxidase, GOD, GOx, Beta-D-glucose:oxygen 1-oxido-reductase, gox

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Functional Studies - Glucose Oxidase Activity Assay Kit (Colorimetric) (AB219924)
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Supplier Data

Functional Studies - Glucose Oxidase Activity Assay Kit (Colorimetric) (AB219924)

Glucose Oxidase Activity Assay Kit (Colorimetric) (ab219924). Typical glucose oxidase dose response curve. Absorbance was measured on a 96-well clear bottom plate using a SpectraMax microplate reader with path check on.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Assay type

Enzyme activity (quantitative)

Assay time

20m

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Glucose Oxidase Activity Assay Kit ab219924 uses a simple colorimetric assay to measure glucose oxidase (GOx) activity in cell and tissue extracts, and biological fluids. The assay can be performed in a convenient 96-well or 384-well plate format and is easily adapted to automation without a separation or wash step.

The glucose oxidase assay protocol uses a red probe to monitor glucose oxidase activity, leading to an increase in absorbance that can be easily detected at OD575 nm.

Glucose oxidase assay protocol summary:
- add samples and standards to wells
- add glucose oxidase reaction mix
- analyze every 2-3 mins with a microplate reader in kinetic mode for at least 10-30 mins at 37°C

Glucose oxidase (notatin, GOx, EC 1.1.3.4) is a dimeric enzyme that catalyzes oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid.

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What's included?

{ "values": { "500Test": { "sellingSize": "500 Test", "publicAssetCode":"ab219924-500Test", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Assay Buffer", "number":"AB219924-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Horseradish Peroxidase (HRP)", "number":"AB219924-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Glucose Oxidase (100 U)", "number":"AB219924-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"DMSO", "number":"AB219924-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Red Dye (light-sensitive)", "number":"AB219924-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Glucose", "number":"AB219924-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucose oxidase also known as GOX is an enzyme that catalyzes the oxidation of beta-D-glucose to glucono delta-lactone releasing hydrogen peroxide as a by-product. This enzyme with a molecular weight of approximately 160 kDa is commonly found in fungi like Aspergillus and Penicillium species. Glucose oxidase is predominantly expressed in the extracellular space of these organisms facilitating its role in glucose metabolism and defense mechanisms. It plays an important role in various biochemical assays including the 'glucose oxidase assay' 'glucose oxidase test' and 'glucose oxidase kit' often used for glucose quantification in diverse samples.
Biological function summary

Glucose oxidase is significant in both metabolic pathways and environmental defense. The enzyme acts as a part of a larger enzymatic reaction complex that includes peroxidase which together help in the glucose oxidase reaction to maintain cellular redox balance. Additionally the production of hydrogen peroxide by glucose oxidase serves as a chemical defense mechanism against microbial infections. This property is harnessed in technical applications making glucose oxidase a valuable tool in biotechnology for both its metabolic impacts and antimicrobial properties.

Pathways

Glucose oxidase is an important enzyme in the carbohydrate metabolism and oxidative stress response pathways. It acts by converting glucose into glucono delta-lactone which can then enter further metabolic processes. The enzyme interacts closely with other proteins particularly those involved in oxidative phosphorylation such as catalase which neutralizes the hydrogen peroxide generated during the glucose oxidase reaction. These interactions help modulate cellular energy production and maintain oxidative homeostasis highlighting its role within broader metabolic networks.

Glucose oxidase activity is relevant to conditions such as diabetes and neurodegenerative diseases. The enzyme's function in glucose monitoring tools plays a critical role in diabetes management where accurate glucose measurement is important. Additionally altered levels of hydrogen peroxide due to dysregulated glucose oxidase activity is implicated in oxidative stress which can contribute to neurodegenerative disorders like Alzheimer's disease. In these contexts the enzyme's interaction with proteins like catalase and superoxide dismutase becomes significant as these proteins aid in mitigating oxidative damage which is central to the pathology of such diseases.
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Product protocols

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Target data

Glucose oxidase catalyzes the oxidation of beta-D-glucose to D-glucono-delta-lactone and hydrogen peroxide in the presence of molecular oxygen. D-glucono-delta-lactone is sequentially hydrolyzed by lactonase to D-gluconic acid, and the resulting hydrogen peroxide is hydrolyzed by catalase to oxygen and water (PubMed : 14188176, PubMed : 14257628, PubMed : 14299649, PubMed : 15450808, PubMed : 2076553, PubMed : 2406261, PubMed : 24283586, PubMed : 28631058, PubMed : 28939970, PubMed : 34500289, PubMed : 7826581, Ref.4, Ref.5). The activity shows high specificity to beta-D-glucose, with very low to no activity towards L-glucose, 2-deoxy-D-glucose, 3-deoxy-D-glucose, 4-deoxy-D-glucose, 5-deoxy-D-glucose, 6-deoxy-D-glucose, 3-O-methyl-D-glucose, 4-O-methyl-D-glucose, 6-O-methyl-D-glucose, 4,6-O-benzylidene-D-glucose, 5-thio-5-deoxy-D-glucose, D-mannose, D-allose, D-galactose, D-fructose, D-arabinose, D-xylose, trehalose, melibiose, L-mannomethylose, lactose, sucrose or 1,5-anhydro-D-glucitol (PubMed : 14188176, PubMed : 14257628, PubMed : 24283586, Ref.5).
See full target information Glucose oxidase
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 12: PubMed37371950

2023

Liposome-Encapsulated Berberine Alleviates Liver Injury in Type 2 Diabetes via Promoting AMPK/mTOR-Mediated Autophagy and Reducing ER Stress: Morphometric and Immunohistochemical Scoring.

Applications

Unspecified application

Species

Unspecified reactive species

Safaa I Khater,Taghreed N Almanaa,Doaa M Abdel Fattah,Tarek Khamis,Mona M Seif,Naief Dahran,Leena S Alqahtani,Mohamed M M Metwally,Mahmoud Mostafa,Raghad A Albedair,Azza I Helal,Manal Alosaimi,Amany Abdel-Rahman Mohamed

Experimental and therapeutic medicine 23:403 PubMed35637648

2022

Circulating miR-15b, Annexin A1, procalcitonin and interleukin-6 levels differentiate children with metabolically unhealthy obesity from those with metabolically healthy obesity: A case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Khalid M Mohany,Osamah Al Rugaie,Osama Al-Wutayd,Mansour Alsharidah,Abdullah Al-Nafeesah

BMC endocrine disorders 21:152 PubMed34344352

2021

Investigation of the levels of circulating miR-29a, miR-122, sestrin 2 and inflammatory markers in obese children with/without type 2 diabetes: a case control study.

Applications

Unspecified application

Species

Unspecified reactive species

Khalid M Mohany,Osamah Al Rugaie,Osama Al-Wutayd,Abdullah Al-Nafeesah

Advanced materials (Deerfield Beach, Fla.) 31:e1900401 PubMed30920710

2019

Wet/Sono-Chemical Synthesis of Enzymatic Two-Dimensional MnO Nanosheets for Synergistic Catalysis-Enhanced Phototheranostics.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Tang,Wenpei Fan,Weizhong Zhang,Zhen Yang,Ling Li,Zhantong Wang,Ya-Ling Chiang,Yijing Liu,Liming Deng,Liangcan He,Zheyu Shen,Orit Jacobson,Maria A Aronova,Albert Jin,Jin Xie,Xiaoyuan Chen
View all publications
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