Glucose Uptake Assay Kit (Fluorometric) ab136956 is a highly sensitive and easy to use non-radioactive kit which can detect glucose uptake as low as 50 pmol/well in a variety of cell types.
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- Journal of translational medicine 22:1932024Ubiquitin-specific protease 14 targets PFKL-mediated glycolysis to promote the proliferation and migration of oral squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingming Zhang,Lou Geng,Yi Tang,Yingying Wang,Youping Zhang,Chujiao Zhu,Hu Lei,Hanzhang Xu,Qi Zhu,Yingli Wu,Wenli GuPubMed 38388430
- Theranostics 13:4333-43552023Kindlin-2 enhances c-Myc translation through association with DDX3X to promote pancreatic ductal adenocarcinoma progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChengmin Liu,Ke Jiang,Yanyan Ding,Aihua Yang,Renwei Cai,Panzhu Bai,Minggang Xiong,Changying Fu,Meiling Quan,Zailin Xiong,Yi Deng,Ruijun Tian,Chuanyue Wu,Ying SunPubMed 37649609
- iScience 26:1068982023A purinergic mechanism underlying metformin regulation of hyperglycemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJared Senfeld,Qianman Peng,Yi Shi,Shenqi Qian,Jianzhong ShenPubMed 37378329
- JCI insight 8:2023GLUT1 is redundant in hypoxic and glycolytic nucleus pulposus cells of the intervertebral disc.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShira N Johnston,Elizabeth S Silagi,Vedavathi Madhu,Duc H Nguyen,Irving M Shapiro,Makarand V RisbudPubMed 36917198
- Cells 11:2022Hypoxia-Inducible Expression of Annexin A6 Enhances the Resistance of Triple-Negative Breast Cancer Cells to EGFR and AR Antagonists.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephen D Williams,Tunde M Smith,LaMonica V Stewart,Amos M SakwePubMed 36230969
- Clinical and translational medicine 12:e6992022Persistent high glucose induced EPB41L4A-AS1 inhibits glucose uptake via GCN5 mediating crotonylation and acetylation of histones and non-histones.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeijie Liao,Naihan Xu,Haowei Zhang,Weifang Liao,Yanzhi Wang,Songmao Wang,Shikuan Zhang,Yuyang Jiang,Weidong Xie,Yaou ZhangPubMed 35184403
- Frontiers in nutrition 8:7063822021Bornlisy Attenuates Colitis-Associated Colorectal Cancer Inhibiting GPR43-Mediated Glycolysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXia Lu,Shuping Qiao,Chen Peng,Wenyue Yan,Zhen Xu,Junxing Qu,Yayi Hou,Shuli Zhao,Ping Chen,Tingting WangPubMed 34869511
- Cells 10:2021Dapagliflozin Restores Impaired Autophagy and Suppresses Inflammation in High Glucose-Treated HK-2 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Xu,Munehiro Kitada,Yoshio Ogura,Haijie Liu,Daisuke KoyaPubMed 34200774
- Frontiers in endocrinology 12:6538192021RBP4 Is Associated With Insulin Resistance in Hyperuricemia-Induced Rats and Patients With Hyperuricemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChan Liu,Xiao-Rong Zhou,Mu-Yao Ye,Xiang-Qing Xu,Yu-Wei Zhang,Hong Liu,Xian-Zhe HuangPubMed 34177800
Key facts
- Detection method
Fluorescent
- Sample types
Adherent cells, Suspension cells
- Assay type
Quantitative
- Reactive species
Mammals
- Assay time
2h
- Sensitivity
= 0.05 nmol/well
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Glucose Uptake Assay Kit (Fluorometric) ab136956 is a highly sensitive and easy to use non-radioactive kit which can detect glucose uptake as low as 50 pmol/well in a variety of cell types.
Key facts
- Detection method
Fluorescent
- Sample types
Adherent cells, Suspension cells
- Assay type
Quantitative
- Reactive species
Mammals
- Assay time
2h
- Assay Platform
Microplate reader
- Sensitivity
= 0.05 nmol/well
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Glucose Uptake Assay Kit (Fluorometric) ab136956 is a highly sensitive and easy to use non-radioactive kit which can detect glucose uptake as low as 50 pmol/well in a variety of cell types.
2-deoxyglucose (2-DG) is used in glucose uptake assay protocols because of its structural similarity to glucose. 2-DG is taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P cannot be further metabolized, and thus accumulates within cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells. In this assay, the accumulated 2-DG6P is enzymatically oxidized and coupled to a probe, which generates fluorescence in the presence of NADPH.
Glucose uptake assay protocol summary:
- prepare cells with suitable glucose starvation / uptake stimulation depending on experimental set-up
- add 2-DG to cells and incubate for 20 mins at 37°C
- wash cells with PBS to remove exogenous 2-DG
- lyse cells with extraction buffer and repeated pipetting
- freeze/thaw lysates and heat at 85°C for 40 min
- cool on ice for 5 min
- add neutralizing buffer, spin and retain supernatant
- add supernatants and standards to wells
- add reaction mix and incubate for 40 min at 37°C
This product is manufactured by BioVision, an Abcam company and was previously called K666 Glucose Uptake Fluorometric Assay Kit. K666-100 is the same size as the 100 test size of ab136956.
If you want a more sensitive assay, we recommend using Glucose Uptake Assay Kit (Colorimetric) (Glucose Uptake Assay Kit (Colorimetric) ab136955), which contains an amplification step that allows the kit to detect < 10 pmol/well.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glucose often referred to as blood sugar is a simple sugar and an essential carbohydrate in biology. It has a molecular mass of 180.16 g/mol and is highly soluble in water. Glucose circulates in the bloodstream and is absorbed by tissues mainly liver muscle and adipose tissue. It serves as a critical energy source and cells use glucose uptake processes to transport glucose across their membranes. Various diagnostic tools and kits such as glucose assay kits and glucose test kits help measure glucose levels in biological samples.
- Biological function summary
Glucose serves as the primary energy substrate for cells providing energy through glycolysis and oxidative phosphorylation. It is not part of any protein complexes but it interacts with numerous enzymes and proteins to regulate metabolic processes. Glucose operates in maintaining homeostasis and the brain relies on it almost exclusively for energy. Glucose assay reagents and glucose detection kits are utilized to quantify glucose concentrations in research studies examining these functions.
- Pathways
Glucose is a central component in glycolysis and the tricarboxylic acid (TCA) cycle. In glycolysis glucose is broken down into pyruvate generating ATP and NADH in the process. This pathway involves key regulatory proteins such as hexokinase and phosphofructokinase. In the TCA cycle glucose metabolites further produce ATP and CO2 involving enzymes like citrate synthase. Glucose uptake assays provide insights into how these pathways operate under various physiological conditions.
- Associated diseases and disorders
Glucose regulation and metabolism are tightly linked to diabetes mellitus and metabolic syndrome. Diabetes mellitus is characterized by impaired glucose uptake and insulin regulation often involving insulin receptor pathways. Persistent high glucose levels lead to complications such as neuropathy and retinopathy. AMP-activated protein kinase (AMPK) plays an important role in metabolic syndrome by affecting glucose uptake and energy balance. Understanding glucose's role in these diseases is central to devising therapeutic strategies and interventions.
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4 product images
Functional Studies - Glucose Uptake Assay Kit (Fluorometric) (ab136956)
Glucose Uptake measured in 3T3-L1 Adipocytes; I = Insulin.
Functional Studies - Glucose Uptake Assay Kit (Fluorometric) (ab136956)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Functional Studies - Glucose Uptake Assay Kit (Fluorometric) (ab136956)
2-DG6P Standard curve (a) and 2-DG uptake in Human adipocytes (b) , HeLa Cells (c) and 3T3-L1 cells (d)
respectively. I=Insulin; P=Phloretin.
Schematic Diagram - Glucose Uptake Assay Kit (Fluorometric) (ab136956)
Assay Procedure.
Accumulated 2-DG6P is enzymatically oxidized and coupled to the picoprobe, which generates fluorescence in the presence of NADPH.
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