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AB273339

Glucosylceramidase Activity Assay Kit (Fluorometric)

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(4 Publications)

Glucosylceramidase Activity Assay Kit (Fluorometric) ab273339 is a quantitative assay for the determination of Glucosylceramidase activity in tissue homogenates and cell lysates. Readout on any fluorometric (Ex/Em=360/445 nm) plate reader.

- Kit includes standard curve for quantitation, and positive control enzyme.
-Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - Glucosylceramidase Activity Assay Kit (Fluorometric) (AB273339)
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Supplier Data

Functional Studies - Glucosylceramidase Activity Assay Kit (Fluorometric) (AB273339)

Example data.

Measurement of Glucosylceramidase in MDA-MB-231 cell lysates (10μg protein), 3T3 cell lysates (5μg protein) and mouse spleen extracts (5μg protein).

Functional Studies - Glucosylceramidase Activity Assay Kit (Fluorometric) (AB273339)
  • FuncS

Supplier Data

Functional Studies - Glucosylceramidase Activity Assay Kit (Fluorometric) (AB273339)

Example data.

4-MU Standard Curve.

Other - Glucosylceramidase Activity Assay Kit (Fluorometric) (AB273339)
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Supplier Data

Other - Glucosylceramidase Activity Assay Kit (Fluorometric) (AB273339)

Representative image of Glucosylceramidase Activity Assay Kit (Fluorometric) ab273339

Components shown from left to right :

- Glucosylceramidase Substrate

- 4-Methyumbelliferone Standard

- Glucosylceramidase Positive Control

- Assay Buffer 25

- Stop Solution V

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Fluorescent

Sample types

Tissue Homogenate, Cell Lysate

Assay type

Enzyme activity

Results type

Quantitative

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Glucosylceramidase Activity Assay Kit (Fluorometric) is a sensitive assay to measure the the activity level of the glucosylceramidase enzyme in a sample by quantifying the fluorescence intensity of a cleaved synthetic substrate in tissue homogenates & cell lysates.

How the assay works

Glucosylceramidase Activity Assay Kit (Fluorometric) uses a synthetic substrate containing a fluorescent molecule, where the glucosylceramidase enzyme cleaves the substrate, releasing the fluorescent molecule which can then be quantified by measuring its fluorescence intensity (Ex/Em=360/445 nm), thus allowing for the determination of the enzyme's activity level in a sample; essentially, the more fluorescent signal detected, the higher the glucosylceramidase activity present.

Assay protocol summary

Glucosylceramidase Activity Assay Kit (Fluorometric) protocol summary
- Prepare all reagents, samples and positive controls.
- Add all samples to the appropriate wells.
- Prepare and add Glucosylceramidase Substrate to the appropriate wells.
- Measure fluorescence y (Ex/Em = 360/445 nm) at 37°C  in end point mode
- Determine Glucosylceramidase activity using equation.

This product is manufactured by BioVision, an Abcam company and was previously called K2003 Glucosylceramidase Activity Assay Kit (Fluorometric). K2003-100 is the same size as the 100 test size of ab273339.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273339-100Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"Assay Buffer 25", "number":"AB273339-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Glucosylceramidase Positive Control", "number":"AB273339-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"Glucosylceramidase Substrate", "number":"AB273339-CMP04", "productcode":"" }, { "size":"1 x 35 µL", "name":"4-Methylumbelliferone Standard", "number":"AB273339-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"Stop Solution V", "number":"AB273339-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GBA also known as glucosylceramidase is a lysosomal enzyme with a molecular mass of approximately 59 kDa. This enzyme breaks down glucosylceramide into glucose and ceramide. GBA is expressed predominantly in tissues with high metabolic activities such as the brain liver and spleen. Its function relies on its catalytic activity where substrates bind to its active site enabling the hydrolysis process necessary for maintaining cellular metabolism.
Biological function summary

GBA plays an important role in sphingolipid metabolism. It participates in the degradation of glycolipids within the lysosome contributing to lipid recycling. It acts independently rather than as a part of a major enzymatic complex. Through its role in degrading glucosylceramide GBA influences cellular homeostasis and bioenergetics ensuring balance in neural and systemic lipid levels.

Pathways

GBA’s enzymatic functions are integral to the glycosphingolipid metabolic pathway. It is involved in the downstream steps of the lysosomal degradation of glycosphingolipids. The pathway operates alongside other important proteins such as beta-glucosidase and CERT-related transfer proteins all of which contribute to membrane lipid organization and signal transduction processes.

GBA mutations are linked with Gaucher disease and Parkinson’s disease. In Gaucher disease deficient GBA activity leads to substrate accumulation resulting in hepatosplenomegaly and other systemic symptoms. Reduced GBA activity is also associated with increased alpha-synuclein aggregation in Parkinson’s disease implicating it in the pathogenesis of neurodegenerative disorders. The enzyme’s function in these diseases highlights its role in maintaining cellular equilibrium and signaling pathways.
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Product protocols

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Target data

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed40459948

2025

Stearoyl-CoA desaturase inhibition normalizes brain lipid saturation, α-synuclein homeostasis, and motor function in mutant Gba1-Parkinson mice.

Applications

Unspecified application

Species

Unspecified reactive species

Silke Nuber,Harrison Hsiang,Esra'a Keewan,Tim E Moors,Sydney J Reitz,Anupama Tiwari,Gary Ph Ho,Elena Su,Wolf Hahn,Marie-Alexandre Adom,Riddhima Pathak,Matthew Blizzard,Sangjune Kim,Han Seok Ko,Xiaoqun Zhang,Per Svenningsson,Dennis J Selkoe,Saranna Fanning

NPJ Parkinson's disease 11:47 PubMed40089519

2025

Lysophosphatidylcholine promoting α-Synuclein aggregation in Parkinson's disease: disrupting GCase glycosylation and lysosomal α-Synuclein degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Mu,Kaiquan Shao,Mingyu Su,Yurong Guo,Yuxiang Qiu,Ruiao Sun,Sihan Sun,Yaoyu Sun,Chenkai Liu,Wei Wang,Xiaoling Qin,Chuanxi Tang

Cell structure and function 49:1-10 PubMed38072450

2023

Lysosomal membrane integrity in fibroblasts derived from patients with Gaucher disease.

Applications

Unspecified application

Species

Unspecified reactive species

Asuka Hamamoto,Natsuki Kita,Siddabasave Gowda B Gowda,Hiroyuki Takatsu,Kazuhisa Nakayama,Makoto Arita,Shu-Ping Hui,Hye-Won Shin

Disease models & mechanisms 16: PubMed37401371

2023

Oxidative stress induces lysosomal membrane permeabilization and ceramide accumulation in retinal pigment epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin R Zhang,Connor S R Jankowski,Rayna Marshall,Rohini Nair,Néstor Más Gómez,Ahab Alnemri,Yingrui Liu,Elizabeth Erler,Julia Ferrante,Ying Song,Brent A Bell,Bailey H Baumann,Jacob Sterling,Brandon Anderson,Sierra Foshe,Jennifer Roof,Hossein Fazelinia,Lynn A Spruce,Jen-Zen Chuang,Ching-Hwa Sung,Anuradha Dhingra,Kathleen Boesze-Battaglia,Venkata R M Chavali,Joshua D Rabinowitz,Claire H Mitchell,Joshua L Dunaief
View all publications
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Product promise

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