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Glutamate Assay Kit ab83389 is a quantitative, addition-only assay with just one 30-min incubation step. Readout on any colorimetric (450 nm) plate reader. Use as a free glutamate assay (does not detect glutamic acid incorporated in peptides or proteins)

-Cited in over 80 publications
-Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.


Images

Functional Studies - Glutamate Assay Kit (AB83389), expandable thumbnail
  • Functional Studies - Glutamate Assay Kit (AB83389), expandable thumbnail

Publications

Key facts

Detection method
Colorimetric
Sample types
Cell Lysate, Other biological fluids, Plasma, Serum, Urine
Assay type
Quantitative
Assay time
40m

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Alternative names

What's included?

100 Test
Components
Assay Buffer 31
1 x 25 mL
Developer Solution III
1 x 1 Vial
Enzyme Mix VIII
1 x 1 Vial
Glutamate Standard
1 x 0.1 mL

Recommended products

Glutamate Assay Kit ab83389 is a quantitative, addition-only assay with just one 30-min incubation step. Readout on any colorimetric (450 nm) plate reader. Use as a free glutamate assay (does not detect glutamic acid incorporated in peptides or proteins)

-Cited in over 80 publications
-Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Detection method
Colorimetric
Sample types
Cell Lysate, Other biological fluids, Plasma, Serum, Urine
Assay type
Quantitative
Assay time
40m
Assay Platform
Microplate reader

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Glutamate Assay Kit ab83389 provides a sensitive detection method of the glutamate in a variety of samples.

The glutamate assay kit will only measure free glutamate levels but not glutamic acid found in the backbone of peptides or proteins.

In the glutamate assay protocol, the enzyme mix recognizes glutamate as a specific substrate leading to proportional color development.The amount of glutamate can therefore be easily quantified by colorimetric spectrophotometry at OD = 450 nm.

Glutamate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at 37°C
- analyze with microplate reader

EDTA plasma may not be used with the ab83389 Glutamate Assay Kit. EDTA can interfere with the assay. Review our to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Glutamate also known as L-glutamic acid is an essential neurotransmitter in the central nervous system. It plays an important role in synaptic transmission and neural communication. Glutamate is a non-essential amino acid with a molecular mass of approximately 147.13 g/mol. It is widely expressed in the brain notably within neurons and glial cells. It facilitates communication between nerve cells by binding to specific glutamate receptors located on postsynaptic neurons. This critical function makes it integral to numerous neural processes.

Biological function summary

Aside from its role in neurotransmission glutamate acts as a precursor to the synthesis of gamma-aminobutyric acid (GABA). It does not tend to form part of a larger protein complex but interacts with multiple receptor complexes such as NMDA and AMPA receptors enabling synaptic plasticity and cognitive functions like learning and memory. Its ability to modulate excitatory signals marks it as a flexible component in neurobiology.

Pathways

Alongside many components glutamate regulates the synaptic plasticity pathways like long-term potentiation and long-term depression. These pathways are important for adaptive neural activities underlying learning and memory formation. Closely linked to this function are proteins such as NMDA receptor subunits which mediate responses to glutamate signaling. Additionally the glutamate-glutamine cycle is significant for neuron-astrocyte interactions helping maintain neurotransmitter pools and detoxify ammonia.

Associated diseases and disorders

Dysfunctions in glutamate signaling relate to both neurodegenerative and psychiatric conditions. Alzheimer's disease involves impaired glutamate signaling which contributes to synaptic loss and cognitive deficits. Affected pathways often involve NMDA receptors making them a target for therapeutic intervention. Similarly in conditions like schizophrenia altered glutamate transmission can affect dopaminergic pathways impacting various neurobehavioral functions. Proteins like glutamate receptors and transporter proteins may become the focal point in understanding and targeting these disorders through diagnostic tools such as glutamate assay kits.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - Glutamate Assay Kit (ab83389), expandable thumbnail
    Image from Sloniecka M et al., PLoS One 10(7), Fig 5a. Doi: 10.1371/journal.pone.0134157. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Functional Studies - Glutamate Assay Kit (ab83389)

    Glutamate levels of central and peripheral keratocytes were determined using Glutamate assay kit (ab83389).

  • Functional Studies - Glutamate Assay Kit (ab83389), expandable thumbnail

    Functional Studies - Glutamate Assay Kit (ab83389)

    Example of Glutamate standard curve obtained using ab83389

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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