Glutamate Assay Kit
4
(2 Reviews)
|
(84 Publications)
Glutamate Assay Kit ab83389 is a quantitative, addition-only assay with just one 30-min incubation step. Readout on any colorimetric (450 nm) plate reader. Use as a free glutamate assay (does not detect glutamic acid incorporated in peptides or proteins)
-Cited in over 80 publications
-Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
View Alternative Names
GLUR, Smp_128940
- FuncS
PubMed
Functional Studies - Glutamate Assay Kit (AB83389)
Glutamate levels of central and peripheral keratocytes were determined using Glutamate assay kit (ab83389).
Image from Sloniecka M et al., PLoS One 10(7), Fig 5a. Doi: 10.1371/journal.pone.0134157. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
- FuncS
Supplier Data
Functional Studies - Glutamate Assay Kit (AB83389)
Example of Glutamate standard curve obtained using ab83389
- Schematic Diagram
Supplier Data
Schematic Diagram - Glutamate Assay Kit (AB83389)
Representative image of Glutamate Assay Kit ab83389
Components shown from left to right :
- Glutamate Standard
- Developer Solution III
- Enzyme Mix VIII
- Assay Buffer 31
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
Product details
Glutamate Assay Kit ab83389 provides a sensitive detection method of the glutamate in a variety of samples.
The glutamate assay kit will only measure free glutamate levels but not glutamic acid found in the backbone of peptides or proteins.
In the glutamate assay protocol, the enzyme mix recognizes glutamate as a specific substrate leading to proportional color development.The amount of glutamate can therefore be easily quantified by colorimetric spectrophotometry at OD = 450 nm.
Glutamate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at 37°C
- analyze with microplate reader
EDTA plasma may not be used with the ab83389 Glutamate Assay Kit. EDTA can interfere with the assay. Review our to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Aside from its role in neurotransmission glutamate acts as a precursor to the synthesis of gamma-aminobutyric acid (GABA). It does not tend to form part of a larger protein complex but interacts with multiple receptor complexes such as NMDA and AMPA receptors enabling synaptic plasticity and cognitive functions like learning and memory. Its ability to modulate excitatory signals marks it as a flexible component in neurobiology.
Pathways
Alongside many components glutamate regulates the synaptic plasticity pathways like long-term potentiation and long-term depression. These pathways are important for adaptive neural activities underlying learning and memory formation. Closely linked to this function are proteins such as NMDA receptor subunits which mediate responses to glutamate signaling. Additionally the glutamate-glutamine cycle is significant for neuron-astrocyte interactions helping maintain neurotransmitter pools and detoxify ammonia.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (84)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 16:5621 PubMed40595567
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Molecular neurodegeneration 20:73 PubMed40542358
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Frontiers in oncology 15:1508819 PubMed40391158
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Journal of experimental & clinical cancer research : CR 44:151 PubMed40390008
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Molecular neurodegeneration 20:51 PubMed40319306
2025
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Synapse (New York, N.Y.) 79:e70018 PubMed40317510
2025
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Oncoimmunology 14:2497172 PubMed40271976
2025
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Science advances 11:eadp8504 PubMed40215316
2025
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Communications biology 8:266 PubMed39979531
2025
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Nature communications 15:10484 PubMed39690148
2024
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