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AB138883

Glutamate Assay Kit (Fluorometric)

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(25 Publications)

Glutamate Assay Kit (Fluorometric) (ab138883) provides a quick and sensitive method for the measurement of glutamate (glutamic acid) in various biological samples.

View Alternative Names

GLUR, Smp_128940

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Functional Studies - Glutamate Assay Kit (Fluorometric) (AB138883)
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Lab

Functional Studies - Glutamate Assay Kit (Fluorometric) (AB138883)

Glutamate measured in human biological fluids (mean of duplicates, +/- SD).

Functional Studies - Glutamate Assay Kit (Fluorometric) (AB138883)
  • FuncS

Lab

Functional Studies - Glutamate Assay Kit (Fluorometric) (AB138883)

Glutamic acid measured in cell lysates showing quantity (nmol) per 106 cells (mean of duplicates, +/- SD).

Functional Studies - Glutamate Assay Kit (Fluorometric) (AB138883)
  • FuncS

Lab

Functional Studies - Glutamate Assay Kit (Fluorometric) (AB138883)

Glutamic acid measured in mouse tissue lysates, showing quantity (nmol) per mg of extracted protein (mean of duplicates, +/- SD).

Functional Studies - Glutamate Assay Kit (Fluorometric) (AB138883)
  • FuncS

Lab

Functional Studies - Glutamate Assay Kit (Fluorometric) (AB138883)

Standard curve (30 minutes incubation) : mean of duplicates (+/- SD), with background reads subtracted.

Functional Studies - Glutamate Assay Kit (Fluorometric) (AB138883)
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PubMed

Functional Studies - Glutamate Assay Kit (Fluorometric) (AB138883)

Intracellular glutamate was measured using ab138883. The concentration of intracellular glutamate in cells, during the exponential phase, grown at different hydrostatic pressures.

Amrani A et al., PLoS One, 9(9). Fig 5a. doi: 10.1371/journal.pone.0106831 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Key facts

Detection method

Fluorescent

Sample types

Urine, Plasma, Tissue, Serum, Other biological fluids, Cell Lysate

Results type

Quantitative

Sensitivity

= 1 µM

Assay time

30m

Assay Platform

Microplate reader

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Product details

Glutamate Assay Kit (Fluorometric) (ab138883) provides a quick and sensitive method for the measurement of glutamate (glutamic acid) in various biological samples.

In the glutamate assay protocol, the coupled enzyme system catalyzes the reaction between L-Glutamic acid and NADP+ to produce NADPH, which is specifically recognized by the NADPH sensor and recycled back to NADP+. During the reaction, a red fluorescence product is produced, which in turn can be detected in a fluorescence microplate reader at Ex/Em = 540/590 nm (range Ex/Em = 530-570 / 590-600 nm).

This assay can detect as little as 1 uM glutamic acid. The signal can also be read by absorbance at OD: 576 ± 5 nm, although the sensitivity of the assay is reduced 10-fold.

The assay is robust, and can be easily adapted to automation without separation step as no wash step is required.

Glutamate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min - 2 hr
- analyze with a microplate reader

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What's included?

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab138883-200Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"NADP", "number":"AB138883-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Enzyme Mix", "number":"AB138883-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Glutamic Acid", "number":"AB138883-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"Dilution Buffer", "number":"AB138883-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"Assay Buffer", "number":"AB138883-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutamate also known as L-glutamic acid is an essential neurotransmitter in the central nervous system. It plays an important role in synaptic transmission and neural communication. Glutamate is a non-essential amino acid with a molecular mass of approximately 147.13 g/mol. It is widely expressed in the brain notably within neurons and glial cells. It facilitates communication between nerve cells by binding to specific glutamate receptors located on postsynaptic neurons. This critical function makes it integral to numerous neural processes.
Biological function summary

Aside from its role in neurotransmission glutamate acts as a precursor to the synthesis of gamma-aminobutyric acid (GABA). It does not tend to form part of a larger protein complex but interacts with multiple receptor complexes such as NMDA and AMPA receptors enabling synaptic plasticity and cognitive functions like learning and memory. Its ability to modulate excitatory signals marks it as a flexible component in neurobiology.

Pathways

Alongside many components glutamate regulates the synaptic plasticity pathways like long-term potentiation and long-term depression. These pathways are important for adaptive neural activities underlying learning and memory formation. Closely linked to this function are proteins such as NMDA receptor subunits which mediate responses to glutamate signaling. Additionally the glutamate-glutamine cycle is significant for neuron-astrocyte interactions helping maintain neurotransmitter pools and detoxify ammonia.

Dysfunctions in glutamate signaling relate to both neurodegenerative and psychiatric conditions. Alzheimer's disease involves impaired glutamate signaling which contributes to synaptic loss and cognitive deficits. Affected pathways often involve NMDA receptors making them a target for therapeutic intervention. Similarly in conditions like schizophrenia altered glutamate transmission can affect dopaminergic pathways impacting various neurobehavioral functions. Proteins like glutamate receptors and transporter proteins may become the focal point in understanding and targeting these disorders through diagnostic tools such as glutamate assay kits.
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Product protocols

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Target data

See full target information Glutamic Acid
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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Materials horizons 12:5302-5314 PubMed40314589

2025

Spiropyran-based glutamate nanovalve for neuronal stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

M Blanco-Formoso,F Galluzzi,F Vacca,T Gianiorio,I Piergentili,A B Cook,P L W Welzen,J C M van Hest,S Di Marco,F Tantussi,F Benfenati,E Colombo,F De Angelis

Frontiers in cell and developmental biology 13:1543710 PubMed40143972

2025

The effect of Polybrominated diphenyl ethers at the fetal blood-brain-barrier: evaluation using a microphysiological system.

Applications

Unspecified application

Species

Unspecified reactive species

Sourabh Sharma,Manuel Vidal,Souvik Paul,Arum Han,Ramkumar Menon,Lauren S Richardson

PloS one 19:e0309728 PubMed39226266

2024

Urinary metabolic profile and its predictive indexes after MSG consumption in rat.

Applications

Unspecified application

Species

Unspecified reactive species

Manatsaphon Sukmak,Thin Su Kyaw,Kanokwan Nahok,Amod Sharma,Atit Silsirivanit,Worachart Lert-Itthiporn,Deanpen Japrung,Somchai Pinlaor,Sirirat Anutrakulchai,Carlo Selmi,Carolyn M Slupsky,Bruce D Hammock,Ubon Cha'on

Applied and environmental microbiology 90:e0114824 PubMed39082806

2024

Nitrate promotes the growth and the production of short-chain fatty acids and tryptophan from commensal anaerobe in the lactate-deficient environment by facilitating the catabolism of glutamate and aspartate.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-He Hung,Shi-Min Zhang,Shir-Ly Huang

Open veterinary journal 14:683-691 PubMed38549576

2024

Suppressive effect of Yokukansan on glutamate released from canine keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yoichiro Kasuga,Ailing Hu,Zenji Kawakami,Masahiro Tabuchi,Takuji Yamaguchi,Hiroyuki Kobayashi,Shigaku Ikeda

Nature neuroscience 27:656-665 PubMed38378993

2024

A phenotypic screening platform for identifying chemical modulators of astrocyte reactivity.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin L L Clayton,James D Kristell,Kevin C Allan,Erin F Cohn,Molly Karl,Andrew D Jerome,Eric Garrison,Yuka Maeno-Hikichi,Annalise M Sturno,Alexis Kerr,H Elizabeth Shick,Jesse A Sepeda,Eric C Freundt,Andrew R Sas,Benjamin M Segal,Robert H Miller,Paul J Tesar

Redox biology 68:102965 PubMed38000344

2023

Thiosulfate sulfurtransferase deficiency promotes oxidative distress and aberrant NRF2 function in the brain.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Luo,Laurent Chatre,Shaden Melhem,Zayana M Al-Dahmani,Natalie Z M Homer,Anneke Miedema,Leo E Deelman,Matthew R Groves,Martin Feelisch,Nicholas M Morton,Amalia Dolga,Harry van Goor

Cell death & disease 14:520 PubMed37582794

2023

m5C-methylated lncRNA NR_033928 promotes gastric cancer proliferation by stabilizing GLS mRNA to promote glutamine metabolism reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Lang Fang,Hongxin Huang,Jialun Lv,Zetian Chen,Chen Lu,Tianlu Jiang,Penghui Xu,Ying Li,Sen Wang,Bowen Li,Zheng Li,Weizhi Wang,Zekuan Xu

Nature communications 14:2502 PubMed37130865

2023

Metabolism-based targeting of MYC via MPC-SOD2 axis-mediated oxidation promotes cellular differentiation in group 3 medulloblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Emma Martell,Helgi Kuzmychova,Esha Kaul,Harshal Senthil,Subir Roy Chowdhury,Ludivine Coudière Morrison,Agnes Fresnoza,Jamie Zagozewski,Chitra Venugopal,Chris M Anderson,Sheila K Singh,Versha Banerji,Tamra E Werbowetski-Ogilvie,Tanveer Sharif

International journal of molecular sciences 24: PubMed37047602

2023

Gut Microbiota Metabolites Differentially Release Gliotransmitters from the Cultured Human Astrocytes: A Preliminary Report.

Applications

Unspecified application

Species

Unspecified reactive species

Michał Seweryn Karbownik,Paulina Sokołowska,Edward Kowalczyk
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