AB102527

Glutamate Dehydrogenase Activity Assay Kit

Name: Glutamate Dehydrogenase Activity Assay Kit (ab102527) | Abcam
Brand: Abcam Limited
SKU: AB102527
Price: 560 USD
Availability: InStock

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(24 Publications)

Glutamate Dehydrogenase Activity Assay Kit (ab102527) provides a convenient tool for sensitive detection of GDH in a variety of samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

GLUD, GLUD1, GDH 1

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Functional Studies - Glutamate Dehydrogenase Activity Assay Kit (AB102527)

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Functional Studies - Glutamate Dehydrogenase Activity Assay Kit (AB102527)

GDH measured in various samples showing O.D.'s in time course

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Supplier Data

Functional Studies - Glutamate Dehydrogenase Activity Assay Kit (AB102527)

NADH Standard Curve

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Lab

Functional Studies - Glutamate Dehydrogenase Activity Assay Kit (AB102527)

GDH measured in cell lysates showing activity (mU) per 10⁶ cells tested

FuncS

Lab

Functional Studies - Glutamate Dehydrogenase Activity Assay Kit (AB102527)

GDH measured showing activity (mU) per mL of tested sample

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Assay type

Enzyme activity

Sensitivity

> 0.01 mU/well

Assay Platform

Microplate reader

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Reactivity data

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Product details

Glutamate Dehydrogenase Activity Assay Kit (ab102527) provides a convenient tool for sensitive detection of GDH in a variety of samples. GDH in sample will consume glutamate as a specific substrate and generate NADH stoichiometrically, resulting in a proportional color development. The GDH activity is easily quantified colorimetrically (450 nm). This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.

Other Notes
This product was previously called K729 Biovision Glutamate Dehydrogenase Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to alpha-Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutamate dehydrogenase (GDH) also known as GDH1 or GLUD1 plays a critical role in ammonia metabolism by catalyzing the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme with a molecular mass of approximately 55 kDa is predominantly expressed in the liver kidney brain and pancreas. It localizes to the mitochondrial matrix indicating its importance in cellular energy production. Researchers often study GDH through various assays like the GDH assay to understand its enzymatic activities and kinetic properties.

Biological function summary

This enzyme facilitates the integration of amino acid and carbohydrate metabolism by regulating glutamate levels and influencing the tricarboxylic acid (TCA) cycle. GDH does not form part of a larger complex but acts independently. Its activity is essential for maintaining the balance between energy supply and demand in cells impacting processes such as insulin secretion in pancreatic beta-cells. Inhibitors of GDH activity like some described by Ari Meneguzzi demonstrate its importance in metabolic regulation.

Pathways

GDH functions in the ammonia detoxification and urea cycle pathways. These pathways involve the conversion of glutamate ensuring efficient nitrogen removal from the body. Key proteins linked to GDH in these pathways include carbamoyl phosphate synthetase and ornithine transcarbamylase as they collectively manage nitrogen flow. GDH also intertwines with glutamine dehydrogenase highlighting its involvement in amino acid metabolism.

GDH is linked to hyperinsulinism-hyperammonemia (HI/HA) syndrome and hepatic encephalopathy. HI/HA syndrome occurs due to mutations in the GDH gene leading to inappropriate insulin secretion. Proteins such as GLUD1 are connected to GDH in the context of HI/HA. In hepatic encephalopathy altered GDH activity contributes to ammonia accumulation affecting brain function. Here GDH interactions with other mitochondrial proteins become important in disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle (PubMed : 11032875, PubMed : 11254391, PubMed : 16023112, PubMed : 16959573). Plays a role in insulin homeostasis (PubMed : 11297618, PubMed : 9571255). May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity).

See full target information GLUD1

Additional targets

GLUD2

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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Archives of microbiology 206:41 PubMed38147133

2023

A robust CRISPR interference gene repression system in Vibrio parahaemolyticus.

Applications

Unspecified application

Species

Unspecified reactive species

Taoyuan Jiang,Yuhuan Li,Wencong Hong,Mingyu Lin

EBioMedicine 85:104311 PubMed36374770

2022

Mitochondrial respiration during normothermic liver machine perfusion predicts clinical outcome.

Applications

Unspecified application

Species

Unspecified reactive species

Andras T Meszaros,Julia Hofmann,Madita L Buch,Benno Cardini,Theresia Dunzendorfer-Matt,Florian Nardin,Michael J Blumer,Margot Fodor,Martin Hermann,Bettina Zelger,Giorgi Otarashvili,Melanie Schartner,Annemarie Weissenbacher,Rupert Oberhuber,Thomas Resch,Jakob Troppmair,Dietmar Fner,Heinz Zoller,Herbert Tilg,Erich Gnaiger,Theresa Hautz,Stefan Schneeberger

Cells 11: PubMed36359822

2022

Glutamate Prevents Altered Mitochondrial Function Following Recurrent Low Glucose in Hypothalamic but Not Cortical Primary Rat Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Paul G Weightman Potter,Kate L J Ellacott,Andrew D Randall,Craig Beall

Biomolecules & therapeutics 30:409-417 PubMed35611585

2022

Sinapic Acid Ameliorates REV-ERB α Modulated Mitochondrial Fission against MPTP-Induced Parkinson's Disease Model.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-Bin Lee,Hyun Ok Yang

Cell reports 39:110660 PubMed35417703

2022

SENP1-Sirt3 signaling promotes α-ketoglutarate production during M2 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhou,Gaolei Hu,Jianli He,Tianshi Wang,Yong Zuo,Ying Cao,Quan Zheng,Jun Tu,Jiao Ma,Rong Cai,Yalan Chen,Qiuju Fan,Baijun Dong,Hongsheng Tan,Qi Wang,Wei Xue,Jinke Cheng

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 42:1410-1424 PubMed35296173

2022

Aging related impairment of brain microvascular bioenergetics involves oxidative phosphorylation and glycolytic pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Siva Svp Sakamuri,Venkata N Sure,Lahari Kolli,Wesley R Evans,Jared A Sperling,Gregory J Bix,Xiaoying Wang,Dmitriy N Atochin,Walter L Murfee,Ricardo Mostany,Prasad Vg Katakam

Life (Basel, Switzerland) 12: PubMed35054496

2022

Alterations in Energy Metabolism, Mitochondrial Function and Redox Homeostasis in GK Diabetic Rat Tissues Treated with Aspirin.

Applications

Unspecified application

Species

Unspecified reactive species

Annie John,Layla Amiri,Jasmin Shafarin,Saeed Tariq,Ernest Adeghate,Frank Christopher Howarth,Haider Raza

Signal transduction and targeted therapy 6:375 PubMed34728602

2021

The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry.

Applications

Unspecified application

Species

Unspecified reactive species

Xujun Liu,Wenzhe Si,Lin He,Jianguo Yang,Yani Peng,Jie Ren,Xiaoping Liu,Tong Jin,Huajing Yu,Zihan Zhang,Xiao Cheng,Wenting Zhang,Lu Xia,Yunchao Huang,Yue Wang,Shumeng Liu,Lin Shan,Yu Zhang,Xiaohan Yang,Haixia Li,Jing Liang,Luyang Sun,Yongfeng Shang

Scientific reports 10:21159 PubMed33273545

2020

SIRT3, a metabolic target linked to ataxia-telangiectasia mutated (ATM) gene deficiency in diffuse large B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kavita Bhalla,Sausan Jaber,Kayla Reagan,Arielle Hamburg,Karen F Underwood,Aditya Jhajharia,Maninder Singh,Binny Bhandary,Shambhu Bhat,Nahid M Nanaji,Ruching Hisa,Carrie McCracken,Heather Huot Creasy,Rena G Lapidus,Tami Kingsbury,Dirk Mayer,Brian Polster,Ronald B Gartenhaus

Molecular vision 26:277-290 PubMed32300272

2020

Proinflammatory cytokines trigger biochemical and neurochemical changes in mouse retinal explants exposed to hyperglycemic conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Gaganashree Shivashankar,Julie C Lim,Monica L Acosta

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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