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AB284547

Glutaminase (GLS) Activity Assay Kit (Fluorometric)

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(2 Publications)

Glutaminase (GLS) Activity Assay Kit ab284547 is a simple plate-based fluorometric assay for measuring glutaminase activity in biological samples.

View Alternative Names

GA, GLS2, GLS, L-glutaminase, L-glutamine amidohydrolase, GLS1, KIAA0838, GLS, K-glutaminase, L-glutamine amidohydrolase

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Functional Studies - Glutaminase (GLS) Activity Assay Kit (Fluorometric) (AB284547)
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Supplier Data

Functional Studies - Glutaminase (GLS) Activity Assay Kit (Fluorometric) (AB284547)

PicoProbe™ Glutaminase (GLS) Activity Assay Kit (Fluorometric).

Glutamate standard curve

Functional Studies - Glutaminase (GLS) Activity Assay Kit (Fluorometric) (AB284547)
  • FuncS

Supplier Data

Functional Studies - Glutaminase (GLS) Activity Assay Kit (Fluorometric) (AB284547)

PicoProbe™ Glutaminase (GLS) Activity Assay Kit (Fluorometric).

Enzyme kinetics for rat brain lysate (3.9 μg protein per well) and rat liver lysate (9.5 μg protein per well)

Functional Studies - Glutaminase (GLS) Activity Assay Kit (Fluorometric) (AB284547)
  • FuncS

Supplier Data

Functional Studies - Glutaminase (GLS) Activity Assay Kit (Fluorometric) (AB284547)

PicoProbe™ Glutaminase (GLS) Activity Assay Kit (Fluorometric).

Glutaminase specific activity in rat brain (GLS1) and liver tissue (GLS2). Experiments were conducted according to kit protocol.

Key facts

Detection method

Fluorescent

Sample types

Tissue Lysate, Cell Lysate

Assay type

Enzyme activity

Assay Platform

Microplate (12 x 8 well strips)

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Primary Antibodies

AB113509

Anti-GLS2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 antibody (AB113509)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This Glutaminase (GLS) Activity Assay Kit (ab284547) (previously called PicoProbe Glutaminase (GLS) Activity Assay Kit K455) is a simple plate-based fluorometric assay for measuring glutaminase activity in biological samples. GLS hydrolyzes glutamine forming glutamate and ammonia. Glutamate, in the presence of a developer and enzyme mix converts a non-fluorescent probe to a fluorescent product via an enzymatic reaction.

The assay can detect as low as 2 uU of glutaminase in biological samples.

Other Notes
This product was previously called K455 Biovision PicoProbeTM Glutaminase (GLS) Activity Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab284547-100Test", "assetComponentDetails": [ { "size":"1 x 0.2 mL", "name":"PicoProbe II", "number":"AB284547-CMP08", "productcode":"" }, { "size":"1 x 10 µL", "name":"GLS Positive Control", "number":"AB284547-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"GLS Dilution Buffer", "number":"AB284547-CMP04", "productcode":"" }, { "size":"1 x 35 mL", "name":"GLS Assay Buffer", "number":"AB284547-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix E", "number":"AB284547-CMP05", "productcode":"" }, { "size":"1 x 0.1 mL", "name":"Glutamate Standard", "number":"AB284547-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Converter Mix A", "number":"AB284547-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"GLS Substrate", "number":"AB284547-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutaminase also known as GLS or GLS1 is an enzyme that catalyzes the conversion of glutamine to glutamate an important step in cellular nitrogen and energy metabolism. This enzyme weighs approximately 65 kDa and is expressed in various tissues particularly in the brain kidney and liver. Glutaminase exists in two isoforms GLS1 and GLS2 with GLS1 being the more studied due to its role in cancer metabolism.
Biological function summary

Glutaminase plays a central role in cellular processes by facilitating the production of glutamate which is an important neurotransmitter in the brain and a precursor for the synthesis of gamma-aminobutyric acid (GABA). It is not part of a larger complex but acts independently in the mitochondrial matrix where it performs the glutaminase reaction. The activity of this enzyme is monitored closely because it affects multiple cellular functions and has implications in many diseases.

Pathways

Glutaminase is instrumental in the glutaminolysis and tricarboxylic acid (TCA) cycles. These pathways are essential for cellular energy production and biosynthesis. GLS1 activity influences other proteins such as alanine transaminase (ALT) and aspartate transaminase (AST) which also play roles in amino acid metabolism and energy production. Glutaminase inhibitors have been explored as potential therapeutic agents by disrupting the glutaminolysis pathway in cancer cells leading to decreased tumor growth.

Glutaminase has a significant link to cancer and neurodegenerative diseases. In cancer GLS1 activity is often upregulated as tumor cells require increased glutamine metabolism for growth and survival making GLS1 inhibitors a target in cancer therapy. Neurodegenerative disorders such as Alzheimer's disease also show altered glutamate levels implicating glutaminase in disease progression. These disorders highlight the enzyme's potential connections with proteins like NMDA receptors in the brain where glutamate acts as a neurotransmitter affecting synaptic activity.
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Product protocols

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Target data

Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
See full target information GLS2

Additional targets

GLS
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC musculoskeletal disorders 26:268 PubMed40091031

2025

Teriparatide facilitates osteogenic differentiation of bone mesenchymal stem cells to alleviate idiopathic osteoporosis via the circFNDC3B-miR-125a-5p-GLS axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxin Fu,Zhi Liu,Guangxin Zhang,Chun Zhang

Cell reports. Medicine 5:101706 PubMed39236712

2024

Combinatorial targeting of glutamine metabolism and lysosomal-based lipid metabolism effectively suppresses glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yaogang Zhong,Feng Geng,Logan Mazik,Xinmin Yin,Aline Paixao Becker,Shabber Mohammed,Huali Su,Enming Xing,Yongjun Kou,Cheng-Yao Chiang,Yunzhou Fan,Yongchen Guo,Qiang Wang,Pui-Kai Li,Xiaokui Mo,Etienne Lefai,Liqing He,Xiaolin Cheng,Xiang Zhang,Arnab Chakravarti,Deliang Guo
View all publications
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websiteProtocolBooklet
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Product promise

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