JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB283389

Glutaminase (GLS1) Inhibitor Screening Kit (Fluorometric)

Be the first to review this product! Submit a review

|

(0 Publication)

GLS1 Inhibitor Screening Kit is a plate-based fluorometric kit for screening human GLS1 inhibitors.

View Alternative Names

GA, GLS2, GLS, L-glutaminase, L-glutamine amidohydrolase, GLS1, KIAA0838, GLS, K-glutaminase, L-glutamine amidohydrolase

2 Images
Functional Studies - Glutaminase (GLS1) Inhibitor Screening Kit (Fluorometric) (AB283389)
  • FuncS

Supplier Data

Functional Studies - Glutaminase (GLS1) Inhibitor Screening Kit (Fluorometric) (AB283389)

Enzyme kinetics in presence and absence of CB-839. Assays were performed using kit protocol

Functional Studies - Glutaminase (GLS1) Inhibitor Screening Kit (Fluorometric) (AB283389)
  • FuncS

Supplier Data

Functional Studies - Glutaminase (GLS1) Inhibitor Screening Kit (Fluorometric) (AB283389)

Inhibition of Glutaminase Activity. IC 50 was determined to be 43.97 ± 20.4 nM.

Key facts

Detection method

Fluorescent

Sample types

Inhibitor compounds

Assay Platform

Microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB83389

Glutamate Assay Kit

Functional Studies - Glutamate Assay Kit (AB83389)

  • 4
  • 2
Assay Kits

AB284572

Glutamine Synthetase Activity Assay Kit (Colorimetric)

Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (AB284572)

  • 0
  • 0
Assay Kits

AB197011

Glutamine Assay Kit (Colorimetric)

Functional Studies - Glutamine Assay Kit (Colorimetric) (AB197011)

  • 3
  • 1
Primary Antibodies

AB113509

Anti-GLS2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 antibody (AB113509)

  • 5
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

GLS1 Inhibitor Screening Kit is a plate-based fluorometric kit for screening human GLS1 inhibitors. The hydrolysis of glutamine by glutaminase forms glutamate and ammonia. Glutamate, in the presence of a developer and enzyme mix, converts a non-fluorescent probe to a fluorescent product via an enzymatic reaction. A well-known Glutaminase inhibitor - CB-839 is provided as an inhibitor control.

This kit is sensitive and is a specific assay for screening GLS1 inhibitors.

This product is manufactured by BioVision, an Abcam company and was previously called K479 Glutaminase (GLS1) Inhibitor Screening Kit (Fluorometric). K479-100 is the same size as the 100 test size of ab283389.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab283389-100Test", "assetComponentDetails": [ { "size":"1 x 0.4 mL", "name":"PicoProbe I", "number":"AB283389-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Glutaminase Assay Buffer", "number":"AB283389-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"GLS Dilution Buffer", "number":"AB283389-CMP07", "productcode":"" }, { "size":"1 x 20 µL", "name":"CB-839 (GLS Inhibitor)", "number":"AB283389-CMP01", "productcode":"" }, { "size":"1 x 20 µL", "name":"Active Glutaminase", "number":"AB283389-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix E", "number":"AB283389-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Converter Mix A", "number":"AB283389-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Glutamine (GLS Substrate)", "number":"AB283389-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutaminase also known as GLS or GLS1 is an enzyme that catalyzes the conversion of glutamine to glutamate an important step in cellular nitrogen and energy metabolism. This enzyme weighs approximately 65 kDa and is expressed in various tissues particularly in the brain kidney and liver. Glutaminase exists in two isoforms GLS1 and GLS2 with GLS1 being the more studied due to its role in cancer metabolism.
Biological function summary

Glutaminase plays a central role in cellular processes by facilitating the production of glutamate which is an important neurotransmitter in the brain and a precursor for the synthesis of gamma-aminobutyric acid (GABA). It is not part of a larger complex but acts independently in the mitochondrial matrix where it performs the glutaminase reaction. The activity of this enzyme is monitored closely because it affects multiple cellular functions and has implications in many diseases.

Pathways

Glutaminase is instrumental in the glutaminolysis and tricarboxylic acid (TCA) cycles. These pathways are essential for cellular energy production and biosynthesis. GLS1 activity influences other proteins such as alanine transaminase (ALT) and aspartate transaminase (AST) which also play roles in amino acid metabolism and energy production. Glutaminase inhibitors have been explored as potential therapeutic agents by disrupting the glutaminolysis pathway in cancer cells leading to decreased tumor growth.

Glutaminase has a significant link to cancer and neurodegenerative diseases. In cancer GLS1 activity is often upregulated as tumor cells require increased glutamine metabolism for growth and survival making GLS1 inhibitors a target in cancer therapy. Neurodegenerative disorders such as Alzheimer's disease also show altered glutamate levels implicating glutaminase in disease progression. These disorders highlight the enzyme's potential connections with proteins like NMDA receptors in the brain where glutamate acts as a neurotransmitter affecting synaptic activity.
style
left-column

Product protocols

style
left-column

Target data

Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
See full target information GLS2

Additional targets

GLS
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com