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AB197011

Glutamine Assay Kit (Colorimetric)

3

(1 Review)

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(52 Publications)

Glutamine Assay kit (Colorimetric) ab197011 is a simple and sensitive assay to quantify the concentration of glutamine in biological fluids and tissues.

- Cited in >35 Publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

Gln

4 Images
Functional Studies - Glutamine Assay Kit (Colorimetric) (AB197011)
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Supplier Data

Functional Studies - Glutamine Assay Kit (Colorimetric) (AB197011)

Measurement of Glutamine concentration in Mouse tissues (brain [15 μg], liver [50 μg]). Samples were deproteinized using 10K Spin Column and spiked with known amount of Glutamine (6 nmol).

Functional Studies - Glutamine Assay Kit (Colorimetric) (AB197011)
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Supplier Data

Functional Studies - Glutamine Assay Kit (Colorimetric) (AB197011)

Glutamine Standard Curve. For demonstrative purposes only.

Functional Studies - Glutamine Assay Kit (Colorimetric) (AB197011)
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Supplier Data

Functional Studies - Glutamine Assay Kit (Colorimetric) (AB197011)

Measurement of Glutamine concentration in Human urine (5 μl).

Schematic Diagram - Glutamine Assay Kit (Colorimetric) (AB197011)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Glutamine Assay Kit (Colorimetric) (AB197011)

Representative image of Glutamine Assay Kit (Colorimetric) ab197011

Components shown from left to right :

- Glutamine Standard

- Developer Solution III

- Enzyme Mix VIII

- Hydrolysis Enzyme Mix II

- Assay Buffer 29

- Assay Buffer 31

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue, Serum, Other biological fluids

Assay type

Quantitative

Sensitivity

= 25 µM

Assay time

90m

Assay Platform

Microplate reader

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Product details

How the assay works

The glutamine assay protocol is based on the hydrolysis of Glutamine to Glutamate producing a stable colored signal, which is directly proportional to the amount of glutamine in the sample.

Glutamine assay protocol summary:

- Add samples and standards to wells
- Add hydrolysis mix and incubate for 30 min
- Add reaction mix and incubate for 60 min
- Analyze with a microplate reader.

How other researchers are using

Glutamine Assay Kit (Colorimetric) has been used in a variety of sample type including:
Human eutopic endometrial and ectopic endometrial tissues 1
Humanpancreatic cancer cell lines (PANC-1 and Capan-1) 2
Human Esophageal squamous cell carcinoma (ESCC) cell lines 3
References:

1-Wu K et al. 2024
2-Kim M et al. 2023
3-Chen L et al. 2023

This product is manufactured by BioVision, an Abcam company and was previously called K556 Glutamine Colorimetric Assay Kit. K556-100 is the same size as the 100 test size of ab197011.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab197011-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Hydrolysis Enzyme Mix II", "number":"AB197011-CMP02", "productcode":"" }, { "size":"1 x 1 Each", "name":"Developer Solution III", "number":"AB197011-CMP04", "productcode":"" }, { "size":"1 x 1 Each", "name":"Glutamine Standard", "number":"AB197011-CMP03", "productcode":"" }, { "size":"1 x 1 Each", "name":"Enzyme Mix VIII", "number":"AB197011-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 31", "number":"AB197011-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 29", "number":"AB197011-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutamine often abbreviated as Gln is an amino acid with a molecular weight of approximately 146.15 g/mol. It serves as a significant nitrogen donor in cellular processes and contributes to synthesizing nucleotides and amino acids. The amino acid is non-essential meaning the body can synthesize it as needed mainly produced in muscles and distributed via the bloodstream to other tissues including the liver brain and immune cells. Glutamine's role in ammonia transportation is notable as it helps in detoxifying ammonium ions. It influences cellular proliferation through its involvement in various cellular functions.
Biological function summary

Glutamine plays an important role in supporting rapid cell division and growth such as in the immune system and intestinal tract. It forms part of the larger metabolic function known as glutaminolysis which provides energy and precursors for biosynthesis. Within cells glutamine works in tandem with several enzymes and transport proteins such as glutamine synthetase which regulates its production and use. The connection with the liver is significant as the organ converts glutamine into glucose and ammonium with glutamate acting as an intermediary.

Pathways

The role of glutamine extends to important areas like the tricarboxylic acid (TCA) cycle and the glutamate-glutamine cycle. In the TCA cycle it serves as a carbon source feeding into gluconeogenesis and other biosynthetic pathways. The conversion to glutamate followed by other downstream metabolites links glutamine closely to amino acid metabolism. Important proteins like glutamate dehydrogenase facilitate these conversions and highlight the interconnected nature of these metabolic pathways.

Abnormalities in glutamine metabolism have associations with conditions such as cancer and neurodegenerative diseases. Cancer cells often exhibit increased glutamine uptake supporting their rapid growth through metabolic pathways like glutaminolysis. On the other hand neurodegenerative diseases involve disruptions in the glutamate-glutamine cycle implicating excitotoxicity-related proteins like NMDA receptors. Altered levels of glutamine and associated proteins in these diseases suggest potential therapeutic targets and diagnostic markers emphasizing its role in cellular health and disease progression.
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Product protocols

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Target data

See full target information Glutamine
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Publications (52)

Recent publications for all applications. Explore the full list and refine your search

Bone research 13:62 PubMed40506462

2025

Chaperone-mediated autophagy directs a dual mechanism to balance premature senescence and senolysis to prevent intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangrong Cheng,Haiyang Gao,Pengzhi Shi,Anran Zhang,Xianglong Chen,Yuhang Chen,Weikang Gan,Kangcheng Zhao,Shuai Li,Cao Yang,Yukun Zhang

Journal of experimental & clinical cancer research : CR 44:151 PubMed40390008

2025

ANXA1 promotes intrahepatic cholangiocarcinoma proliferation and growth by regulating glutamine metabolism through GOT1 stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyu Gong,Liwen Chen,Hao Wang,Dijie Zheng,Futang Li,Changhao Wu,Yongning Li,Yazhu Deng,Zhiwei He,Chao Yu

Acta biochimica et biophysica Sinica 57:1093-1105 PubMed39696988

2024

ATF4 promotes glutaminolysis and glycolysis in colorectal cancer by transcriptionally inducing SLC1A5.

Applications

Unspecified application

Species

Unspecified reactive species

Zengli Zhou,Shufang Ye,Jingyu Chen,Fei Dai,Luyi Chen,Ran Ye,Jianmei Zhang,Gefei Chen,Yanjiao Wang,Yangyang Liu

The clinical respiratory journal 18:e13825 PubMed39187936

2024

TFAP2A Activates S100A2 to Mediate Glutamine Metabolism and Promote Lung Adenocarcinoma Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zeng,Wangsheng Ren,Hang Zeng,Dachun Wang,Xianyu Wu,Guo Xu

American journal of cancer research 14:3153-3170 PubMed39005663

2024

LncRNA FEZF1-AS1 facilitates cisplatin resistance in non-small cell lung cancer through modulating the miR-32-5p-glutaminase axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Lin,Wei-Chun Wu,Zhi Liang,Jian-Hao Zhang,Shi-Peng Fang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2401748 PubMed38994891

2024

Overcoming Cancer Persister Cells by Stabilizing the ATF4 Promoter G-quadruplex.

Applications

Unspecified application

Species

Unspecified reactive species

Chengmei Xiao,Yipu Li,Yushuang Liu,Ruifang Dong,Xiaoyu He,Qing Lin,Xin Zang,Kaibo Wang,Yuanzheng Xia,Lingyi Kong

The Journal of biological chemistry 300:107473 PubMed38879007

2024

Dietary protein load affects the energy and nitrogen balance requiring liver glutamate dehydrogenase to maintain physical activity.

Applications

Unspecified application

Species

Unspecified reactive species

Karolina Luczkowska,Yan Zhou,Angela M Ramos-Lobo,Thierry Brun,Pierre Maechler

Experimental and therapeutic medicine 27:218 PubMed38590564

2024

SLC38A2 promotes cell proliferation and invasion by promoting glutamine metabolism in adenomyosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kui Wu,Wei Gong,Jia-Chang Hu,Ying-Chun Duan,Hui-Hui Ke,Li Chen

Journal of advanced research 68:341-357 PubMed38432394

2024

Baicalein induces apoptosis by inhibiting the glutamine-mTOR metabolic pathway in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyang Li,Di Zhang,Shaohui Wang,Peng Yu,Jiayi Sun,Yi Zhang,Xianli Meng,Juan Li,Li Xiang

Thoracic cancer 15:764-777 PubMed38400818

2024

CircCPA4 induces ASCT2 expression to promote tumor property of non-small cell lung cancer cells in a miR-145-5p-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhua Zhang,Weiliang Liu,Tao Huang,Junyan Li,Hui Hu,Xinyu Xu,Zhigang Fan
View all publications
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