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AB284572

Glutamine Synthetase Activity Assay Kit (Colorimetric)

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(2 Publications)

Glutamine Synthetase Activity Assay Kit (Colorimetric) (ab284572) is a simple, plate-based colorimetric assay for measuring Glutamine Synthetase activity in biological samples.

View Alternative Names

Glutamine-dependent NAD(+) synthetase, NAD(+) synthase [glutamine-hydrolyzing], NAD(+) synthetase, NADSYN1, GLNS, GLUL, Glutamine synthetase, GS, Glutamate--ammonia ligase, Palmitoyltransferase GLUL

4 Images
Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (AB284572)
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Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (AB284572)

Glutamine Sythetase Specific Activity in Rat Brain Extracts.

Glutamine Synthetase specific activity in rat brain (3.3 μg), rat liver (7.2 μg) and 3T3 cell lysates (14 μg). All assays were performed following kit protocols.

Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (AB284572)
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Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (AB284572)

Enzyme kinetics of rat brain extracts (3.3 μg).

Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (AB284572)
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Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (AB284572)

ADP Standard Curve Example

Other - Glutamine Synthetase Activity Assay Kit (Colorimetric) (AB284572)
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Supplier Data

Other - Glutamine Synthetase Activity Assay Kit (Colorimetric) (AB284572)

Representative image of Glutamine Synthetase Activity Assay Kit (Colorimetric) ab284572

Components shown from left to right :

- OxiRed Probe

- Converter Mix II

- ADP Standard

- Developer Mix A

- ATP V (2 vials)

- Glutamate

- GS Assay Buffer I

- Glutamine Synthetase

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Cell Lysate

Assay type

Enzyme activity

Sensitivity

>= 10 µU

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Glutamine Synthetase Activity Assay Kit (Colorimetric) (ab284572) is a simple, plate-based colorimetric assay for measuring Glutamine Synthetase activity in biological samples.

How the assay works
In the Glutamine Synthetase assay, Glutamine Synthetase hydrolyzes Glutamate to Glutamine and ADP. ADP in the subsequent enzymatic reaction, in presence of ADP Converter, ADP Developer Mix and ADP Probe forms a colorimetric product that is measured at OD 570 nm.

Glutamine Synthetase assay protocol summary
- Add samples and standards to wells
- Add reaction mix and incubate
- Analyze with microplate reader at 570 nm in kinetic mode for 60 min at 37°C

Other Notes
This product was previously called K2056 Biovision Glutamine Synthetase Activity Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutamine synthetase also known as glutamine s synthetase or glnA is an enzyme that catalyzes the ATP-dependent conversion of glutamate and ammonia into glutamine. This reaction plays an important role in nitrogen metabolism. Glutamine synthetase has a molecular weight of approximately 620 kDa and forms a multimeric structure commonly seen in bacteria plants and animal tissues with significant expression in the brain liver and kidneys.
Biological function summary

This enzyme supports the detoxification of ammonia by incorporating it into glutamine an essential amino acid and nitrogen donor. Glutamine synthetase operates independently rather than as part of a larger protein complex. It assists in maintaining cellular nitrogen balance and facilitates the synthesis of proteins and other nitrogen-containing molecules. Glutamine peptides serve vital roles in cellular processes underlining the significance of their synthesis.

Pathways

Glutamine synthetase integrates into the glutamate and glutamine cycle between neurons and glial cells highlighting its part in neurotransmitter metabolism. It also features prominently in the urea cycle influencing nitrogen disposal in organisms. Glutamine synthetase interacts with glutaminase which assists in transforming glutamine back to glutamate maintaining a balance of nitrogenous compounds within these pathways.

Glutamine synthetase abnormalities link to hepatic encephalopathy and neurodegenerative disorders such as Alzheimer's disease. Altered enzyme expression contributes to increased ammonia levels adversely affecting brain function. In Alzheimer's disease connections with tau and amyloid-beta proteins suggest a link between glutamine synthetase activity and neurotoxic events. Understanding these interactions may offer insights into therapeutic approaches for these conditions.
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Product protocols

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Target data

Catalyzes the final step of the nicotinamide adenine dinucleotide (NAD) de novo synthesis pathway, the ATP-dependent amidation of deamido-NAD using L-glutamine as a nitrogen source.
See full target information NADSYN1

Additional targets

GLUL
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of human genetics 111:729-741 PubMed38579670

2024

Clustered de novo start-loss variants in GLUL result in a developmental and epileptic encephalopathy via stabilization of glutamine synthetase.

Applications

Unspecified application

Species

Unspecified reactive species

Amy G Jones,Matilde Aquilino,Rory J Tinker,Laura Duncan,Zandra Jenkins,Gemma L Carvill,Stephanie J DeWard,Dorothy K Grange,M J Hajianpour,Benjamin J Halliday,Muriel Holder-Espinasse,Judit Horvath,Silvia Maitz,Vincenzo Nigro,Manuela Morleo,Victoria Paul,Careni Spencer,Alina I Esterhuizen,Tilman Polster,Alice Spano,Inés Gómez-Lozano,Abhishek Kumar,Gemma Poke,John A Phillips,Hunter R Underhill,Gregory Gimenez,Takashi Namba,Stephen P Robertson

Nature communications 14:6949 PubMed37914686

2023

A carbon-nitrogen negative feedback loop underlies the repeated evolution of cnidarian-Symbiodiniaceae symbioses.

Applications

Unspecified application

Species

Unspecified reactive species

Guoxin Cui,Jianing Mi,Alessandro Moret,Jessica Menzies,Huawen Zhong,Angus Li,Shiou-Han Hung,Salim Al-Babili,Manuel Aranda
View all publications
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Product promise

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