Glutamine Synthetase Activity Assay Kit (Colorimetric) (ab284572) (K2056) is a simple, plate-based colorimetric assay for measuring GS activity in biological samples.
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- Nature communications 14:69492023A carbon-nitrogen negative feedback loop underlies the repeated evolution of cnidarian-Symbiodiniaceae symbioses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuoxin Cui,Jianing Mi,Alessandro Moret,Jessica Menzies,Huawen Zhong,Angus Li,Shiou-Han Hung,Salim Al-Babili,Manuel ArandaPubMed 37914686
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Cell Lysate
- Assay type
Enzyme activity
- Reactive species
Mammals
- Sensitivity
>= 10 µU
Target data
Function
Catalyzes the final step of the nicotinamide adenine dinucleotide (NAD) de novo synthesis pathway, the ATP-dependent amidation of deamido-NAD using L-glutamine as a nitrogen source.
Targets
GLNA_HUMAN
Alternative names
Glutamine-dependent NAD(+) synthetase, NAD(+) synthase [glutamine-hydrolyzing], NAD(+) synthetase, NADSYN1
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Glutamine Synthetase Activity Assay Kit (Colorimetric) (ab284572) (K2056) is a simple, plate-based colorimetric assay for measuring GS activity in biological samples.
Alternative names
Glutamine-dependent NAD(+) synthetase, NAD(+) synthase [glutamine-hydrolyzing], NAD(+) synthetase, NADSYN1
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Cell Lysate
- Assay type
Enzyme activity
- Reactive species
Mammals
- Assay Platform
Microplate (12 x 8 well strips)
- Sensitivity
>= 10 µU
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Glutamine Synthetase Activity Assay Kit (Colorimetric) (ab284572) (K2056) is a simple, plate-based colorimetric assay for measuring GS activity in biological samples. In this assay, GS hydrolyzes Glutamate to Glutamine and ADP. ADP in the subsequent enzymatic reaction, in presence of ADP Converter, ADP Developer Mix and ADP Probe forms a colorimetric product that is measured at OD 570 nm. The assay is simple, sensitive and can detect as low as 10 μU of GS in biological samples.
This product is manufactured by BioVision, an Abcam company and was previously called K2056 Glutamine Synthetase Activity Assay Kit (Colorimetric). K2056-100 is the same size as the 100 test size of ab284572.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glutamine synthetase also known as glutamine s synthetase or glnA is an enzyme that catalyzes the ATP-dependent conversion of glutamate and ammonia into glutamine. This reaction plays an important role in nitrogen metabolism. Glutamine synthetase has a molecular weight of approximately 620 kDa and forms a multimeric structure commonly seen in bacteria plants and animal tissues with significant expression in the brain liver and kidneys.
- Biological function summary
This enzyme supports the detoxification of ammonia by incorporating it into glutamine an essential amino acid and nitrogen donor. Glutamine synthetase operates independently rather than as part of a larger protein complex. It assists in maintaining cellular nitrogen balance and facilitates the synthesis of proteins and other nitrogen-containing molecules. Glutamine peptides serve vital roles in cellular processes underlining the significance of their synthesis.
- Pathways
Glutamine synthetase integrates into the glutamate and glutamine cycle between neurons and glial cells highlighting its part in neurotransmitter metabolism. It also features prominently in the urea cycle influencing nitrogen disposal in organisms. Glutamine synthetase interacts with glutaminase which assists in transforming glutamine back to glutamate maintaining a balance of nitrogenous compounds within these pathways.
- Associated diseases and disorders
Glutamine synthetase abnormalities link to hepatic encephalopathy and neurodegenerative disorders such as Alzheimer's disease. Altered enzyme expression contributes to increased ammonia levels adversely affecting brain function. In Alzheimer's disease connections with tau and amyloid-beta proteins suggest a link between glutamine synthetase activity and neurotoxic events. Understanding these interactions may offer insights into therapeutic approaches for these conditions.
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3 product images
Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (ab284572)
ADP Standard Curve Example
Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (ab284572)
Enzyme kinetics of rat brain extracts (3.3 μg).
Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (ab284572)
Glutamine Sythetase Specific Activity in Rat Brain Extracts.
Glutamine Synthetase specific activity in rat brain (3.3 μg), rat liver (7.2 μg) and 3T3 cell lysates (14 μg). All assays were performed following kit protocols.
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