Glutamine Synthetase Activity Assay Kit (Colorimetric) (ab284572) is a simple, plate-based colorimetric assay for measuring Glutamine Synthetase activity in biological samples.
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- Nature communications 14:69492023A carbon-nitrogen negative feedback loop underlies the repeated evolution of cnidarian-Symbiodiniaceae symbioses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuoxin Cui,Jianing Mi,Alessandro Moret,Jessica Menzies,Huawen Zhong,Angus Li,Shiou-Han Hung,Salim Al-Babili,Manuel ArandaPubMed 37914686
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell Lysate
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Sensitivity
- >= 10 µU
Target data
Function
Catalyzes the final step of the nicotinamide adenine dinucleotide (NAD) de novo synthesis pathway, the ATP-dependent amidation of deamido-NAD using L-glutamine as a nitrogen source.
Additional Targets
GLUL
Alternative names
Glutamine-dependent NAD(+) synthetase, NAD(+) synthase [glutamine-hydrolyzing], NAD(+) synthetase, NADSYN1
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Glutamine Synthetase Activity Assay Kit (Colorimetric) (ab284572) is a simple, plate-based colorimetric assay for measuring Glutamine Synthetase activity in biological samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell Lysate
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- >= 10 µU
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Glutamine Synthetase Activity Assay Kit (Colorimetric) (ab284572) is a simple, plate-based colorimetric assay for measuring Glutamine Synthetase activity in biological samples.
How the assay works
In the Glutamine Synthetase assay, Glutamine Synthetase hydrolyzes Glutamate to Glutamine and ADP. ADP in the subsequent enzymatic reaction, in presence of ADP Converter, ADP Developer Mix and ADP Probe forms a colorimetric product that is measured at OD 570 nm.
Glutamine Synthetase assay protocol summary
- Add samples and standards to wells
- Add reaction mix and incubate
- Analyze with microplate reader at 570 nm in kinetic mode for 60 min at 37°C
Other Notes
This product was previously called K2056 Biovision Glutamine Synthetase Activity Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glutamine synthetase also known as glutamine s synthetase or glnA is an enzyme that catalyzes the ATP-dependent conversion of glutamate and ammonia into glutamine. This reaction plays an important role in nitrogen metabolism. Glutamine synthetase has a molecular weight of approximately 620 kDa and forms a multimeric structure commonly seen in bacteria plants and animal tissues with significant expression in the brain liver and kidneys.
- Biological function summary
This enzyme supports the detoxification of ammonia by incorporating it into glutamine an essential amino acid and nitrogen donor. Glutamine synthetase operates independently rather than as part of a larger protein complex. It assists in maintaining cellular nitrogen balance and facilitates the synthesis of proteins and other nitrogen-containing molecules. Glutamine peptides serve vital roles in cellular processes underlining the significance of their synthesis.
- Pathways
Glutamine synthetase integrates into the glutamate and glutamine cycle between neurons and glial cells highlighting its part in neurotransmitter metabolism. It also features prominently in the urea cycle influencing nitrogen disposal in organisms. Glutamine synthetase interacts with glutaminase which assists in transforming glutamine back to glutamate maintaining a balance of nitrogenous compounds within these pathways.
- Associated diseases and disorders
Glutamine synthetase abnormalities link to hepatic encephalopathy and neurodegenerative disorders such as Alzheimer's disease. Altered enzyme expression contributes to increased ammonia levels adversely affecting brain function. In Alzheimer's disease connections with tau and amyloid-beta proteins suggest a link between glutamine synthetase activity and neurotoxic events. Understanding these interactions may offer insights into therapeutic approaches for these conditions.
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3 product images
Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (ab284572)
ADP Standard Curve Example
Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (ab284572)
Enzyme kinetics of rat brain extracts (3.3 μg).
Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (ab284572)
Glutamine Sythetase Specific Activity in Rat Brain Extracts.
Glutamine Synthetase specific activity in rat brain (3.3 μg), rat liver (7.2 μg) and 3T3 cell lysates (14 μg). All assays were performed following kit protocols.
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