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AB219926

Glutathione Peroxidase Activity Assay Kit (Fluorometric)

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(6 Publications)

Glutathione Peroxidase Activity Assay Kit (Fluorometric) (ab219926) provides a simple method to measure Glutathione Peroxidase activity in cell lysates.
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Functional Studies - Glutathione Peroxidase Activity Assay Kit (Fluorometric) (AB219926)
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Supplier Data

Functional Studies - Glutathione Peroxidase Activity Assay Kit (Fluorometric) (AB219926)

Typical Glutathione Peroxidase dose response curve. Fluorescence was measured on a solid black 96-well plate using a Gemini microplate reader (Molecular Devices). As low as 1.25 mU/mL GPx can be detected with 30-60 minutes incubation.

Key facts

Detection method

Fluorescent

Sample types

Cell Lysate

Assay type

Enzyme activity

Sensitivity

< 1.25 mU/mL

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Glutathione Peroxidase Activity Assay Kit (Fluorometric) (ab219926) provides a simple method to measure Glutathione Peroxidase activity in cell lysates. This assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG) catalyzed by Glutathione Peroxidase (GPx). The generated GSSG is recycled to its reduced state GSH by glutathione reductase (GR) and NADPH. The reaction product, NADP+, can be specifically monitored using our newly developed proprietary NADP sensor, which reacts with NADP only to generate a fluorescent product. The signal, which is proportional to the GPx activity present in the sample, can be measured with a fluorescence microplate reader at Ex/Em = 420/480 nm.

This assay can detect activity from as low as 1.25 mU/mL Glutathione Peroxidase in solution. The assay can be performed in a convenient 96-well or 384-well plate format and is easily adapted to automation without a separation or wash step.

Glutathione peroxidase (GPx) is an enzyme family with peroxidase activity to protect the organism from oxidative damage. GPx plays an important role in reducing organic hydroperoxides such as lipid hydroperoxides to their corresponding alcohols, or reducing free hydrogen peroxide to water. It therefore guards against oxidative damage to the cell membranes and other oxidant-sensitive sites in the cell. It has been noticed that altered GPx levels correlate with lesions caused by many comment and complex diseases. GPx level is measured in biological samples as a potential indicator for the potential treatment of cancer, diabetes, neurodegenerative and cardiovascular diseases.

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What's included?

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab219926-200Test", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"NADP Assay Solution", "number":"AB219926-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Enzyme Mix", "number":"AB219926-CMP07", "productcode":"" }, { "size":"1 x 11 µL", "name":"Substrate", "number":"AB219926-CMP08", "productcode":"" }, { "size":"1 x 5 mL", "name":"NADP Probe", "number":"AB219926-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Glutathione Peroxidase Standard (0.5 U)", "number":"AB219926-CMP01", "productcode":"" }, { "size":"1 x 3.5 mL", "name":"Enhancer Solution", "number":"AB219926-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"Assay Buffer", "number":"AB219926-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"GSH (3 mg)", "number":"AB219926-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione Peroxidase (GPx) often referred to as GSH peroxidase is an essential enzyme in the cellular defense system against oxidative damage. It exists as a group of enzymes including the widely studied isoform GPx4. GPx has a molecular weight of approximately 20-23 kDa varying slightly depending on the isoform. This enzyme is localized in many tissues across different organs including the liver kidney and muscles. It catalyzes the reduction of hydrogen peroxide and organic hydroperoxides using glutathione (GSH) as a substrate preventing the formation of free radicals.
Biological function summary

The enzyme plays a significant role in the detoxification of reactive oxygen species. GPx does not usually form a complex with other proteins; instead it acts individually within cells to maintain the balance of oxidative and reductive forces. By converting harmful peroxides into non-toxic alcohols and water it protects cell membranes and other cellular components from oxidative damage. GPx activity is often measured in laboratories using assays to determine its efficiency in catalyzing these reactions.

Pathways

GPx engages in critical cellular antioxidant defense mechanisms. It is an integral part of the glutathione redox cycle where it partners with glutathione reductase another enzyme important for regenerating reduced glutathione. Additionally GPx links to the pentose phosphate pathway providing reduced NADPH necessary for maintaining glutathione in its reduced form. These pathways protect cells from oxidative stress and keep redox homeostasis in check.

Alterations in GPx activity correlate with various health conditions. Researchers link decreased GPx activity to cardiovascular diseases and neurodegenerative disorders highlighting its importance in maintaining cellular health. In cardiovascular diseases the imbalance of oxidative stress pathways involves proteins like superoxide dismutase in addition to GPx worsening the effects of oxidative damage. In neurodegenerative disorders abnormal protein aggregations disturb GPx activities emphasizing the role of antioxidants in disease prevention and management.
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Product protocols

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Target data

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Chemical biology & drug design 105:e70142 PubMed40556500

2025

Apelin-13 Protects Against Airway Inflammation, Oxidative Stress, and Epithelial-Mesenchymal Transition in a Chronic Asthma Model.

Applications

Unspecified application

Species

Unspecified reactive species

Huifeng Sun,Ai Wang,Nan Shi

Antioxidants (Basel, Switzerland) 13: PubMed38671839

2024

Selenium Deficiency Exacerbates Hyperoxia-Induced Lung Injury in Newborn C3H/HeN Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lora C Bailey-Downs,Laura G Sherlock,Michaela N Crossley,Aristides Rivera Negron,Paul T Pierce,Shirley Wang,Hua Zhong,Cynthia Carter,Kathryn Burge,Jeffrey V Eckert,Lynette K Rogers,Peter F Vitiello,Trent E Tipple

International journal of molecular sciences 24: PubMed36835090

2023

Genistein and Procyanidin B2 Reduce Carcinogen-Induced Reactive Oxygen Species and DNA Damage through the Activation of Nrf2/ARE Cell Signaling in Bronchial Epithelial Cells In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Tharindu L Suraweera,J P Jose Merlin,Graham Dellaire,Zhaolin Xu,H P Vasantha Rupasinghe

Nanomaterials (Basel, Switzerland) 11: PubMed34578640

2021

Light-Activated Protoporphyrin IX-Based Polysilsesquioxane Nanoparticles Induce Ferroptosis in Melanoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hemapriyadarshini Vadarevu,Ridhima Juneja,Zachary Lyles,Juan L Vivero-Escoto

Scientific reports 11:6135 PubMed33731759

2021

The medium-chain fatty acid decanoic acid reduces oxidative stress levels in neuroblastoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Janine Mett,Uli Müller

Nature communications 10:4400 PubMed31562333

2019

Connective tissue fibroblasts from highly regenerative mammals are refractory to ROS-induced cellular senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Sandeep Saxena,Hemendra Vekaria,Patrick G Sullivan,Ashley W Seifert
View all publications
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