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Glutathione Peroxidase Assay Kit (Colorimetric) ab102530 can be used to quantitate the activity of all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.


Images

Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (AB102530), expandable thumbnail
  • Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (AB102530), expandable thumbnail
  • Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (AB102530), expandable thumbnail
  • Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (AB102530), expandable thumbnail
  • Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (AB102530), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Platelets, Other biological fluids

Assay type

Enzyme activity

Reactive species

Mammals

Assay time

40m

Sensitivity

= 0.5 mU/mL

Associated Products

Select an associated product type

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Alternative names

What's included?

2000 Test
Components
Active Glutathione Peroxidase
20 x 1 Vial
Active Glutathione Reductase
20 x 1 Vial
Cumene Hydroperoxide
20 x 1 Vial
Glutathione
20 x 1 Vial
Glutathione Peroxidase Assay Buffer
20 x 50 mL
NADPH II
20 x 1 Vial

Recommended products

Glutathione Peroxidase Assay Kit (Colorimetric) ab102530 can be used to quantitate the activity of all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Platelets, Other biological fluids

Assay type

Enzyme activity

Reactive species

Mammals

Assay time

40m

Assay Platform

Microplate reader

Sensitivity

= 0.5 mU/mL

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Glutathione Peroxidase Assay Kit (Colorimetric) ab102530 can be used to quantitate the activity of all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.

In the glutathione peroxidase assay protocol, glutathione peroxidase (GPx) oxidizes GSH to produce GSSG as part of the reaction in which it reduces cumene hydroperoxide. Glutathione reductase (GR) then reduces the GSSG to produce GSH, and in the same reaction consumes NADPH. The decrease of NADPH (measured at OD=340 nm) is proportional to GPx activity.

The assay has a detection sensitivity of ~ 0.5 mU/ml of GPx in samples.

Glutathione peroxidase assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 15 min at room temp to deplete all GSSG in sample
- add cumene hydroperoxide
- analyze with microplate reader immediately, and after at least 5 min

This product is manufactured by BioVision, an Abcam company and was previously called K762 Glutathione Peroxidase Activity Colorimetric Assay Kit. K762-100 is the same size as the 100 test size of ab102530.

The Glutathione Peroxidase (GPx, EC 1.11.1.9) family of enzymes plays and important role in the protection of organisms from oxidative damage. GPx converts reduced glutathione (GSH) to oxidized glutathione (GSSG) while reducing lipid hydroperoxides to their corresponding alcohols or free hydrogen peroxide to water.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Glutathione Peroxidase (GPx) often referred to as GSH peroxidase is an essential enzyme in the cellular defense system against oxidative damage. It exists as a group of enzymes including the widely studied isoform GPx4. GPx has a molecular weight of approximately 20-23 kDa varying slightly depending on the isoform. This enzyme is localized in many tissues across different organs including the liver kidney and muscles. It catalyzes the reduction of hydrogen peroxide and organic hydroperoxides using glutathione (GSH) as a substrate preventing the formation of free radicals.

Biological function summary

The enzyme plays a significant role in the detoxification of reactive oxygen species. GPx does not usually form a complex with other proteins; instead it acts individually within cells to maintain the balance of oxidative and reductive forces. By converting harmful peroxides into non-toxic alcohols and water it protects cell membranes and other cellular components from oxidative damage. GPx activity is often measured in laboratories using assays to determine its efficiency in catalyzing these reactions.

Pathways

GPx engages in critical cellular antioxidant defense mechanisms. It is an integral part of the glutathione redox cycle where it partners with glutathione reductase another enzyme important for regenerating reduced glutathione. Additionally GPx links to the pentose phosphate pathway providing reduced NADPH necessary for maintaining glutathione in its reduced form. These pathways protect cells from oxidative stress and keep redox homeostasis in check.

Associated diseases and disorders

Alterations in GPx activity correlate with various health conditions. Researchers link decreased GPx activity to cardiovascular diseases and neurodegenerative disorders highlighting its importance in maintaining cellular health. In cardiovascular diseases the imbalance of oxidative stress pathways involves proteins like superoxide dismutase in addition to GPx worsening the effects of oxidative damage. In neurodegenerative disorders abnormal protein aggregations disturb GPx activities emphasizing the role of antioxidants in disease prevention and management.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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6 product images

  • Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530), expandable thumbnail
    George, Akash K et al., Scientific reports?vol. 8,1 5158., Fig 4, doi:10.1038/s41598-018-23568-z

    Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530)

    Scatter dot plots representing the levels of glutathione peroxidase (GPx) in brain tissue in different mice groups.

  • Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530), expandable thumbnail
    Liu, Baohai et al., Frontiers in pharmacology?vol. 9 347., Fig 8, doi:10.3389/fphar.2018.00347

    Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530)

    The effects of PCSE on the activity of GPX in a mouse UC model.

  • Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530), expandable thumbnail

    Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530)

    Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530), expandable thumbnail

    Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530)

    Glutathione Peroxidase Activity measured in biological fluids showing quantity (nmol) per 1 mL after 10 min of incubation. Samples diluted 1-27 fold.

  • Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530), expandable thumbnail

    Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530)

    Glutathione Peroxidase Activity measured in cell lysates showing quantity (nmol) per 1 mln cells after 10 min of incubation. Undiluted sample contained 2.5e6 cells per well. Samples were diluted 1-9 fold.

  • Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530), expandable thumbnail

    Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530)

    Tests example obtained using ab102530

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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