Glutathione Reductase Assay Kit (ab83461) is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples.
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- Nature communications 14:69372023A genome-scale metabolic model of parasitic whipworm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesÖmer F Bay,Kelly S Hayes,Jean-Marc Schwartz,Richard K Grencis,Ian S RobertsPubMed 37907472
- PloS one 18:e02903102023Monitoring physiological processes of fast-growing broilers during the whole life cycle: Changes of redox-homeostasis effected to trassulfuration pathway predicting the development of non-alcoholic fatty liver disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeorgina Pesti-Asbóth,Endre Szilágyi,Piroska Bíróné Molnár,János Oláh,László Babinszky,Levente Czeglédi,Zoltán Cziáky,Melinda Paholcsek,László Stündl,Judit RemenyikPubMed 37590293
- Foods (Basel, Switzerland) 12:2023Quercetin-Induced Glutathione Depletion Sensitizes Colorectal Cancer Cells to Oxaliplatin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinkyung Lee,Chan Ho Jang,Yoonsu Kim,Jisun Oh,Jong-Sang KimPubMed 37107528
- Nutrients 15:2023Human Milk Composition and Nutritional Status of Omnivore Human Milk Donors Compared with Vegetarian/Vegan Lactating Mothers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNoelia Ureta-Velasco,Kristin Keller,Diana Escuder-Vieco,Javier Fontecha,María V Calvo,Javier Megino-Tello,José C E Serrano,Carmen Romero Ferreiro,Nadia Raquel García-Lara,Carmen R Pallás-AlonsoPubMed 37111074
- Scientific reports 12:212132022Gum Arabic nanoformulation rescues neuronal lesions in bromobenzene-challenged rats by its antioxidant, anti-apoptotic and cytoprotective potentials.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHailah M Almohaimeed,Hanan Waly,Nasser S Abou Khalil,Khaled M A Hassanein,Basal Sulaiman M Alkhudhairy,Elham A Abd-AllahPubMed 36481816
- Current issues in molecular biology 44:5390-54042022Cardamom Extract Alleviates the Oxidative Stress, Inflammation and Apoptosis Induced during Acetaminophen-Induced Hepatic Toxicity via Modulating Nrf2/HO-1/NQO-1 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEssraa A R Alkhalifah,Amjad A Alobaid,Marwah A Almajed,Manar K Alomair,Lama S Alabduladheem,Sarah F Al-Subaie,Abdullah Akbar,Mahesh V Attimarad,Nancy S Younis,Maged E MohamedPubMed 36354677
- Frontiers in plant science 13:9791412022Ultrasonication affects the melatonin and auxin levels and the antioxidant system in potato .Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeorgina Pesti-Asbóth,Piroska Molnár-Bíróné,Ildikó Forgács,Judit Remenyik,Judit DobránszkiPubMed 36247572
- Life (Basel, Switzerland) 12:2022Ameliorative Effect of D-Carvone against Hepatic Ischemia-Reperfusion-Induced Injury in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaged E Mohamed,Nancy S YounisPubMed 36294936
- Journal of neurotrauma 39:1075-10892022Advanced Age and Neurotrauma Diminish Glutathione and Impair Antioxidant Defense after Spinal Cord Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew N Stewart,Ethan P Glaser,Caitlin A Mott,William M Bailey,Patrick G Sullivan,Samir P Patel,John C GenselPubMed 35373589
- Biotech (Basel (Switzerland)) 11:2022Effects of Intramuscular Injections of Vitamins AD3E and C in Combination on Fertility, Immunity, and Proteomic and Transcriptomic Analyses of Dairy Cows during Early Gestation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWirot Likittrakulwong,Pisit Poolprasert,Worawatt Hanthongkul,Sittiruk RoytrakulPubMed 35822793
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate
- Assay type
Enzyme activity
- Range
0.1 - 40 mU/mL
- Assay time
40m
- Sensitivity
> 0.1 mU/mL
Target data
Function
Maintains high levels of reduced glutathione in the cytosol.
Alternative names
GR, GRase, GSR, GRD1, GLUR
What's included?
Recommended products
Glutathione Reductase Assay Kit (ab83461) is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples.
Alternative names
GR, GRase, GSR, GRD1, GLUR
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate
- Assay type
Enzyme activity
- Range
0.1 - 40 mU/mL
- Assay time
40m
- Assay Platform
Microplate reader
- Sensitivity
> 0.1 mU/mL
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Glutathione Reductase Assay Kit (ab83461) is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5'-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.
Since Glutathione Reductase has significantly higher concentrations in cells (mM range) compared to Thioredoxin Reductase (μM range), we predict that ab83461 will detect mostly GR activity in samples.
Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. **Related products** Review the to learn about more assays for oxidative stress.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glutathione reductase also known as GSR or GSH reductase is an enzyme critical for maintaining the cellular redox balance by reducing glutathione disulfide (GSSG) to its sulfhydryl form GSH. It has a molecular mass of approximately 100 kDa and is widely expressed in various tissues with high levels found in the liver and red blood cells. This enzyme is a flavoprotein that requires FAD as a cofactor to drive the reduction process playing an important role in the recycling of glutathione which is essential for detoxification processes in the cell.
- Biological function summary
Glutathione reductase activity supports cellular defense against oxidative stress by regenerating reduced glutathione. It is not part of a larger complex but interacts with other antioxidants to protect cells from damage. The enzyme works continuously to keep the intracellular environment balanced by maintaining an adequate GSH/GSSG ratio. This balance is vital for neutralizing reactive oxygen species (ROS) which can otherwise cause cellular damage and contribute to disease development.
- Pathways
Glutathione reductase function is integral to the glutathione metabolism and pentose phosphate pathways. It collaborates closely with related proteins like glutathione peroxidase which uses GSH to reduce hydrogen peroxide therefore preventing its toxic accumulation. By ensuring a steady supply of GSH glutathione reductase supports the antioxidant defenses necessary for cellular survival under stress conditions linking it to various signaling cascades that monitor and manage oxidative stress levels.
- Associated diseases and disorders
Glutathione reductase has significant implications in conditions characterized by oxidative stress such as Alzheimer's disease and cardiovascular diseases. Impairments in glutathione reductase activity can lead to insufficient detoxification of ROS contributing to the pathophysiology of these conditions. In Alzheimer's the enzyme's dysfunction allows for increased oxidative damage often involving interactions with other proteins such as amyloid-beta which exacerbates neuronal damage. Similarly in cardiovascular diseases disrupted glutathione homeostasis can drive oxidative stress-related damage to vascular tissues.
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6 product images
Cao, Zeyu et al., Scientific reports?vol. 7,1 16278., Fig 5, doi:10.1038/s41598-017-16446-7Functional Studies - Glutathione Reductase (GR) Assay Kit (ab83461)
ICAC decreased the ROS level induced by EV71 infection via modulating the antioxidant enzymes involved in GSH metabolism. Infected (100 TCID50 EV71) Vero cells were treated with medium or 100 µM ICAC for 12 h. Uninfected cells were used as the control group. The antioxidant enzymes activities of the Vero cells were detected (n = 3). All results were expressed as the means ± SEs. Asterisks indicate that the data significantly differ from the EV71 group at the P < 0.05 level according to one-way analysis of variance.
Functional Studies - Glutathione Reductase (GR) Assay Kit (ab83461)
Glutathione reductase measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1=2 min; T2=30 min).
Functional Studies - Glutathione Reductase (GR) Assay Kit (ab83461)
Glutathione reductase measured in cell lysates (mU) per 106 cells (T1=2 min; T2=30 min)
Functional Studies - Glutathione Reductase (GR) Assay Kit (ab83461)
Glutathione reductase measured in human plasma and serum (T1=2 min; T2=30 min) showing activity (mU) per ml of tested sample. No activity was detected in saliva or urine.
Functional Studies - Glutathione Reductase (GR) Assay Kit (ab83461)
TNB Standard Curve using ab83461.
Functional Studies - Glutathione Reductase (GR) Assay Kit (ab83461)
Functional Studies - Glutathione Reductase Assay Kit (ab83461) Glutathione Reductase assay time line using ab83461.
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