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AB273344

Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric)

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(3 Publications)

Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric) (ab273344) is a robust assay for the measurement of Glyceraldehyde 3-Phosphate (GA3P) in biological samples.
3 Images
Functional Studies - Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric) (AB273344)
  • FuncS

Supplier Data

Functional Studies - Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric) (AB273344)

Example data.

Interpolation of kinetic data for mouse kidney lysate. Δ GA3P = (GA3P spiked – GA3P unspiked).

Functional Studies - Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric) (AB273344)
  • FuncS

Supplier Data

Functional Studies - Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric) (AB273344)

Example data.

GA3P in rat muscle and mouse kidney lysates.

Functional Studies - Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric) (AB273344)
  • FuncS

Supplier Data

Functional Studies - Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric) (AB273344)

Example data.

Kinetics of enzymatic reaction to measure GA3P in rat muscle (608 ng) and mouse kidney (8.5 μg) lysate with and without GA3P Standard spikes.

Key facts

Detection method

Fluorescent

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Quantitative

Assay Platform

Microplate

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Reactivity data

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Product details

Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric) (ab273344) is a robust assay for the measurement of Glyceraldehyde 3-Phosphate (GA3P) in biological samples. The GA3P developer oxidizes GA3P with the release of NADH, which is used by the GA3P enzyme mix to convert the non-fluorescent GA3P probe to a fluorescent product measured at Ex/Em = 535/587 nm.

This product is manufactured by BioVision, an Abcam company and was previously called K2018 Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric). K2018-100 is the same size as the 100 test size of ab273344.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glyceraldehyde 3-phosphate also known as G3P or triose phosphate is a three-carbon sugar phosphate molecule involved in several metabolic processes. It has a molecular mass of about 170.1 g/mol. Glyceraldehyde 3-phosphate is expressed in various tissues where glycolysis and gluconeogenesis occur. As an intermediate in these metabolic pathways glyceraldehyde 3-phosphate plays a critical role in converting glucose molecules into energy.
Biological function summary

Glyceraldehyde 3-phosphate is essential in energy production and biosynthesis. It participates in the glycolytic pathway where it is a substrate for the enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH). This enzyme facilitates its conversion to 13-bisphosphoglycerate playing a part in ATP generation. Additionally glyceraldehyde 3-phosphate exists in the Calvin Cycle of photosynthesis in plants acting in the formation of glucose from carbon dioxide.

Pathways

Glyceraldehyde 3-phosphate is an important component in glycolysis and gluconeogenesis. These pathways involve its interaction with enzymes like triose phosphate isomerase and GAPDH. In glycolysis its conversion helps generate ATP and pyruvate for further energy production. In gluconeogenesis it contributes to glucose production from non-carbohydrate sources. Glyceraldehyde 3-phosphate also links to the pentose phosphate pathway connecting energy production to nucleotide synthesis.

Abnormal metabolism of glyceraldehyde 3-phosphate links to conditions such as cancer and diabetes. Cancer cells often exhibit altered glycolysis (known as the Warburg effect) where glyceraldehyde 3-phosphate levels can impact cell proliferation. In diabetes impaired glucose metabolism affects glyceraldehyde 3-phosphate processing leading to energy deficits. In these conditions proteins like GAPDH are affected influencing disease progression.
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Product protocols

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Target data

See full target information Glyceraldehyde 3-Phosphate
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 26:552-566 PubMed38561547

2024

Glucose controls lipolysis through Golgi PtdIns4P-mediated regulation of ATGL.

Applications

Unspecified application

Species

Unspecified reactive species

Lianggong Ding,Florian Huwyler,Fen Long,Wu Yang,Jonas Binz,Kendra Wernlé,Matthias Pfister,Manuel Klug,Miroslav Balaz,Barbara Ukropcova,Jozef Ukropec,Chunyan Wu,Tongtong Wang,Min Gao,Pierre-Alain Clavien,Philipp Dutkowski,Mark W Tibbitt,Christian Wolfrum

Nature 617:386-394 PubMed37100912

2023

A druggable copper-signalling pathway that drives inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Stéphanie Solier,Sebastian Müller,Tatiana Cañeque,Antoine Versini,Arnaud Mansart,Fabien Sindikubwabo,Leeroy Baron,Laila Emam,Pierre Gestraud,G Dan Pantoș,Vincent Gandon,Christine Gaillet,Ting-Di Wu,Florent Dingli,Damarys Loew,Sylvain Baulande,Sylvère Durand,Valentin Sencio,Cyril Robil,François Trottein,David Péricat,Emmanuelle Näser,Céline Cougoule,Etienne Meunier,Anne-Laure Bègue,Hélène Salmon,Nicolas Manel,Alain Puisieux,Sarah Watson,Mark A Dawson,Nicolas Servant,Guido Kroemer,Djillali Annane,Raphaël Rodriguez

International journal of biological sciences 18:3668-3675 PubMed35813468

2022

IL-6/STAT3 Axis Activates Glut5 to Regulate Fructose Metabolism and Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoke Huang,Jing Fang,Weiqi Lai,Yu Hu,Liang Li,Yuanyou Zhong,Shiwei Yang,Dan He,Rui Liu,Qingfeng Tang
View all publications
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