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Glycerol-3-Phosphate Dehydrogenase (G3PDH) Assay Kit (Colorimetric) (ab174095) is a product where G3PDH reacts with the substrate to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.
Alternative names=Glycerol-3-phosphate dehydrogenase 1-like protein, GPD1-L, GPD1L, KIAA0089
Colorimetric
Tissue Lysate, Cell Lysate
Enzyme activity (quantitative)
Mammals
< 1 mU/well
Glycerol-3-Phosphate Dehydrogenase (G3PDH) Assay Kit (Colorimetric) (ab174095) is a product where G3PDH reacts with the substrate to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.
Alternative names=Glycerol-3-phosphate dehydrogenase 1-like protein, GPD1-L, GPD1L, KIAA0089
Colorimetric
Tissue Lysate, Cell Lysate
Enzyme activity (quantitative)
Mammals
Microplate reader
< 1 mU/well
Blue Ice
-20°C
-20°C
-20°C
Glycerol-3-Phosphate Dehydrogenase (G3PDH) Assay Kit (Colorimetric) (ab174095) is a product where G3PDH reacts with the substrate to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and rapid and can detect Glycerol-3-Phosphate dehydrogenase activity less than 1 mU/well.
Visit our FAQs page for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called K640 Glycerol-3-Phosphate Dehydrogenase Activity Colorimetric Assay Kit. K640-100 is the same size as the 100 test size of ab174095.
Glycerol-3-Phosphate Dehydrogenase (EC 1.1.1.8) is an important enzyme for lipid metabolism. It catalyzes the reversible conversion between dihydroxyacetone phosphate and glycerol-3-phosphate. GPDH plays multiple functions inside cells; it links carbohydrate and lipid metabolism, and provides electrons through the Glycerol-3-Phosphate Shuttle. When progenitor adipocytes differentiate into mature adipocytes, GPDH activity increases significantly. Analysis of glycerol-3-phosphate dehydrogenase activity is crucial for the study of fatty acid metabolic pathways.
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