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Glycerol-3-Phosphate Dehydrogenase (G3PDH) Assay Kit (Colorimetric) (ab174095) is a product where G3PDH reacts with the substrate to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.

Detection method

Colorimetric

Sample types

Tissue Lysate, Cell Lysate

Assay type

Enzyme activity (quantitative)

Reactive species

Mammals

Sensitivity

< 1 mU/well

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Shipping To:
United States

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Target data

What's included?

100 Test
Components
GPDH Assay Buffer
1 x 27 mL
GPDH Positive Control
1 x 1 Vial
GPDH Probe
1 x 1 Vial
GPDH Substrate
1 x 1 Vial
NADH Standard
1 x 1 Vial

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Glycerol-3-Phosphate Dehydrogenase (G3PDH) Assay Kit (Colorimetric) (ab174095) is a product where G3PDH reacts with the substrate to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Cell Lysate

Assay type

Enzyme activity (quantitative)

Reactive species

Mammals

Assay Platform

Microplate reader

Sensitivity

< 1 mU/well

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Glycerol-3-Phosphate Dehydrogenase (G3PDH) Assay Kit (Colorimetric) (ab174095) is a product where G3PDH reacts with the substrate to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and rapid and can detect Glycerol-3-Phosphate dehydrogenase activity less than 1 mU/well.

Visit our FAQs page for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called K640 Glycerol-3-Phosphate Dehydrogenase Activity Colorimetric Assay Kit. K640-100 is the same size as the 100 test size of ab174095.

Glycerol-3-Phosphate Dehydrogenase (EC 1.1.1.8) is an important enzyme for lipid metabolism. It catalyzes the reversible conversion between dihydroxyacetone phosphate and glycerol-3-phosphate. GPDH plays multiple functions inside cells; it links carbohydrate and lipid metabolism, and provides electrons through the Glycerol-3-Phosphate Shuttle. When progenitor adipocytes differentiate into mature adipocytes, GPDH activity increases significantly. Analysis of glycerol-3-phosphate dehydrogenase activity is crucial for the study of fatty acid metabolic pathways.

Product promise

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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