Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric)
Be the first to review this product! Submit a review
|
(4 Publications)
- FuncS
Supplier Data
Functional Studies - Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric) (AB174094)
Measurement of G3P in various samples.
Measurement of G3P in rat liver (100 μg), 3T3-L1 (40 μg) and Hela (50 μg) lysate. This is example data.
- FuncS
Supplier Data
Functional Studies - Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric) (AB174094)
G3P Standard Curve.
This is example data only.
- Schematic Diagram
Supplier Data
Schematic Diagram - Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric) (AB174094)
Representative image of Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric) ab174094
Components shown from left to right :
- G3P Standard
- Developer Solution III
- Developer Mix F
- Assay Buffer 5
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
Product details
Glycerol-3-Phosphate Assay kit (Colorimetric) (ab174094) is a sensitive, fast and easy-to-use kit. In this assay, G3P is oxidized by G3P Enzyme Mix to form an intermediate, which reduces a nearly colorless probe to a colored product with strong absorbance at 450 nm. This assay kit can detect G3P less than 2 nmol/well and can be used for a variety of sample types.
Other Notes
This product was previously called K641 Glycerol-3-Phosphate (G3P) Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.
Glycerol-3-phosphate (G3P) is an important intermediate for all living organisms. Glycerol-3-Phosphate is produced either by glycerol via glycerol kinase or by dihydroxyacetone phosphate through glycerol-3-phosphate dehydrogenase. In response to cellular signals, glycerol-3-phosphate can be utilized in multiple pathways: it can be further converted into glyceraldehyde-3-phosphate and enter glycolysis or rapidly generate NAD+ in brain or muscle tissues through the G3P shuttle or enter the lipid biosynthetic pathway. Recent studies have found that glycerol-3-phosphate is a novel regulator and plays a fundamental defense role in plant pathogenesis.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The conversion of glycerol to glycerol-3-phosphate is an important step in various cellular functions. G3P plays a central role in triglyceride and phospholipid biosynthesis. It serves as a building block for the glycerol backbone in lipid molecules. In cellular respiration G3P is part of the glycerol-3-phosphate shuttle a mechanism in mitochondria that assists in transferring reducing equivalents from cytosolic NADH into the mitochondrial matrix linking G3P with the aerobic respiration process. This shuttle is not a complex by itself but it involves interactions with other mitochondrial components.
Pathways
Glycerol-3-phosphate is deeply entrenched in lipid metabolism and gluconeogenesis. It is an important player in the triglyceride biosynthesis pathway where it is esterified to form phosphatidic acid with acyl-CoAs. This process links it with enzymes such as acyl-CoA synthetase and phosphatidate phosphatase. Additionally the glycerol-3-phosphate shuttle pathway connects G3P to oxidative phosphorylation linking it to enzymes like mitochondrial glycerol-3-phosphate dehydrogenase and ubiquinone which are essential for ATP production.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Publications (4)
Recent publications for all applications. Explore the full list and refine your search
Proceedings of the National Academy of Sciences of the United States of America 122:e2505263122 PubMed40440069
2025
Applications
Unspecified application
Species
Unspecified reactive species
Signal transduction and targeted therapy 10:26 PubMed39848960
2025
Applications
Unspecified application
Species
Unspecified reactive species
Journal of hematology & oncology 15:93 PubMed35836291
2022
Applications
Unspecified application
Species
Unspecified reactive species
Cancer research 80:2150-2162 PubMed32179514
2020
Applications
Unspecified application
Species
Unspecified reactive species
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com