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Glycerol-3-Phosphate Assay kit (Colorimetric) (ab174094) is a sensitive, fast and easy-to-use kit.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

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Images

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Cell Lysate

Assay type

Quantitative

Reactive species

Mammals

Sensitivity

< 2 nmol/well

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What's included?

100 Test
Components
Assay Buffer V
1 x 25 mL
Developer Solution III
1 x 1 Vial
Development Enzyme Mix VII
1 x 1 Vial
G3P Standard
1 x 1 Vial

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Glycerol-3-Phosphate Assay kit (Colorimetric) (ab174094) is a sensitive, fast and easy-to-use kit.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Cell Lysate

Assay type

Quantitative

Reactive species

Mammals

Assay Platform

Microplate reader

Sensitivity

< 2 nmol/well

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Abcam's Glycerol-3-Phosphate Assay kit (Colorimetric) (ab174094) is a sensitive, fast and easy-to-use kit. In this assay, G3P is oxidized by G3P Enzyme Mix to form an intermediate, which reduces a nearly colorless probe to a colored product with strong absorbance at 450 nm. This assay kit can detect G3P less than 2 nmol/well and can be used for a variety of sample types.

Visit our FAQs page for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called K641 Glycerol-3-Phosphate (G3P) Colorimetric Assay Kit. K641-100 is the same size as the 100 test size of ab174094.

Glycerol-3-phosphate (G3P) is an important intermediate for all living organisms. Glycerol-3-Phosphate is produced either by glycerol via glycerol kinase or by dihydroxyacetone phosphate through glycerol-3-phosphate dehydrogenase. In response to cellular signals, glycerol-3-phosphate can be utilized in multiple pathways: it can be further converted into glyceraldehyde-3-phosphate and enter glycolysis or rapidly generate NAD+ in brain or muscle tissues through the G3P shuttle or enter the lipid biosynthetic pathway. Recent studies have found that glycerol-3-phosphate is a novel regulator and plays a fundamental defense role in plant pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric) (ab174094), expandable thumbnail

    Functional Studies - Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric) (ab174094)

    G3P Standard Curve.

    This is example data only.

  • Functional Studies - Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric) (ab174094), expandable thumbnail

    Functional Studies - Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric) (ab174094)

    Measurement of G3P in various samples.

    Measurement of G3P in rat liver (100 μg), 3T3-L1 (40 μg) and Hela (50 μg) lysate. This is example data.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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