Glycine Assay Kit (Fluorometric) (ab211100) provides a provides a simple, sensitive, and high-throughput adaptable assay that detects physiological concentrations of glycine (GLY) in multiple biological samples such as cell and tissue lysates and biological fluids.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- The Journal of clinical investigation 134:2024Impaired glycine neurotransmission causes adolescent idiopathic scoliosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolu Wang,Ming Yue,Jason Pui Yin Cheung,Prudence Wing Hang Cheung,Yanhui Fan,Meicheng Wu,Xiaojun Wang,Sen Zhao,Anas M Khanshour,Jonathan J Rios,Zheyi Chen,Xiwei Wang,Wenwei Tu,Danny Chan,Qiuju Yuan,Dajiang Qin,Guixing Qiu,Zhihong Wu,Terry Jianguo Zhang,Shiro Ikegawa,Nan Wu,Carol A Wise,Yong Hu,Keith Dip Kei Luk,You-Qiang Song,Bo GaoPubMed 37962965
- Nature communications 14:9492023Time-restricted feeding promotes muscle function through purine cycle and AMPK signaling in Drosophila obesity models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher Livelo,Yiming Guo,Farah Abou Daya,Vasanthi Rajasekaran,Shweta Varshney,Hiep D Le,Stephen Barnes,Satchidananda Panda,Girish C MelkaniPubMed 36810287
- Microorganisms 9:2021Identifying a Novel Bile Salt Hydrolase from the Keystone Gut Bacterium .Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuillaume Déjean,Héloïse Tudela,Lisa Bruno,Déborah Kissi,Georges Rawadi,Sandrine P ClausPubMed 34207623
- Cell reports 34:1088692021The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichelle Grace Acoba,Ebru S Selen Alpergin,Santosh Renuse,Lucía Fernández-Del-Río,Ya-Wen Lu,Oleh Khalimonchuk,Catherine F Clarke,Akhilesh Pandey,Michael J Wolfgang,Steven M ClaypoolPubMed 33730581
- Nature communications 7:135012016Inhibition of glycine transporter-1 in the dorsal vagal complex improves metabolic homeostasis in diabetes and obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica T Y Yue,Mona A Abraham,Paige V Bauer,Mary P LaPierre,Peili Wang,Frank A Duca,Beatrice M Filippi,Owen Chan,Tony K T LamPubMed 27874011
Key facts
- Detection method
Fluorescent
- Sample types
Tissue Lysate, Saliva, Urine, Plasma, Serum, Cell Lysate
- Assay type
Quantitative
- Reactive species
Mammals
- Sensitivity
= 1 µM
Target data
What's included?
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Glycine Assay Kit (Fluorometric) (ab211100) provides a provides a simple, sensitive, and high-throughput adaptable assay that detects physiological concentrations of glycine (GLY) in multiple biological samples such as cell and tissue lysates and biological fluids.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Fluorescent
- Sample types
Tissue Lysate, Saliva, Urine, Plasma, Serum, Cell Lysate
- Assay type
Quantitative
- Reactive species
Mammals
- Assay Platform
Microplate reader
- Sensitivity
= 1 µM
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Glycine Assay Kit (Fluorometric) (ab211100) provides a provides a simple, sensitive, and high-throughput adaptable assay that detects physiological concentrations of glycine (GLY) in multiple biological samples such as cell and tissue lysates and biological fluids. In this assay, Glycine is oxidized in the presence of GLY Enzyme Mix is converted to an intermediate, which reacts with the fluorescent probe to generate a strong stable signal at Ex/Em = 535/587 nm. The intensity of the signal is directly proportional to the amount of GLY in the sample.
The reaction is specific and other amino acids do not interfere with the assay. The assay can detect as little as 1 μM of Glycine in a variety of biological samples.
This product is manufactured by BioVision, an Abcam company and was previously called K589 Glycine Assay Kit (Fluorometric). K589-100 is the same size as the 100 test size of ab211100.
Glycine (GLY, G) is one of the 20 standard amino acids commonly found in proteins. Glycine's side chain is a hydrogen substituent, which makes it the smallest proteogenic and the only non-chiral amino acid. Basic functions of Glycine include the participation in the synthesis of creatine, glutathione, heme groups, and conjugated bile acids (bile salts). It is also present as one of the most abundant residues in the triple-helical structure of collagen.
Glycine acts as a glucogenic amino acid by regulating sugar levels in blood. Therefore, glycine supplementation has been used in patients suffering anemia, hypoglycemia and chronic fatigue. Glycine possesses both inhibitory and excitatory neurotransmitter functions in the brain stem and spinal cord. In cancer cells, glycine consumption is highly correlated to cancer cell proliferation via purine synthesis. Glycine uptake in cancer cell studies supports the role of this amino acid in tumorigenesis and malignancy.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glycine a simple amino acid plays a critical role in various biological processes. It is often referred to as Gly or by its chemical formula C2H5NO2. Glycine has a molecular mass of approximately 75.07 g/mol. It is ubiquitously expressed in many tissues but is especially abundant in the central nervous system and connective tissues. Glycine acts as a building block for proteins and serves as an essential neurotransmitter in the central nervous system.
- Biological function summary
Glycine influences the function of neurotransmitter systems. It serves as an inhibitory neurotransmitter in the spinal cord brainstem and retina through glycine receptors. Glycine forms part of collagen complexes in connective tissue contributing to structure and stability. Additionally glycine participates in the synthesis of other biomolecules like purines which are necessary for nucleic acid structure. Glycine's low molecular weight and small size enable it to fit into spaces within protein structures enhancing flexibility and function.
- Pathways
Glycine influences the central nervous system's glycinergic pathways and is integral to the biosynthesis of serine and the glutathione pathway. Glycine's involvement in the glycinergic pathway associates it with glycine receptors and transporters that modulate synaptic transmissions. Within the glutathione pathway glycine works alongside glutamate and cysteine to form glutathione a vital antioxidant. This action highlights its cooperative role with proteins like the glutamate-cysteine ligase in cellular oxidative stress defense.
- Associated diseases and disorders
Glycine shows relevance to conditions such as glycine encephalopathy and schizophrenia. Excess glycine accumulation due to enzyme defects can lead to glycine encephalopathy impacting the central nervous system. Glycine concentrations and related receptor functions may also affect schizophrenia where neurotransmitter systems are disrupted. Glycine's involvement with NMDA receptors has been studied in these disorders suggesting its potential as a target for treatment strategies.
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2 product images
Functional Studies - Glycine Assay Kit (Fluorometric) (ab211100)
Typical Glycine standard calibration curve.
Functional Studies - Glycine Assay Kit (Fluorometric) (ab211100)
Estimation of Glycine in human serum, saliva, and urine. Samples were deproteinized using >10 kD spin column (10kD Spin Column ab93349) and diluted with GLY Assay Buffer prior to performing assay: (serum 1:64 dilution; saliva 1:32 dilution; urine 1:128 dilution). 25 μL of each diluted sample was spiked with 0.3 nmol of Glycine Standard and assayed following the kit protocol. Glycine concentrations in serum (224 ± 21μM), saliva (149 ± 7 μM) and urine (54 ± 4 μM/mM creatinine).
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