AB211100

Glycine Assay Kit (Fluorometric)

Name: Glycine Assay Kit (Fluorometric) (ab211100) | Abcam
Brand: Abcam Limited
SKU: AB211100
Price: 755 USD
Availability: InStock

Be the first to review this product! Submit a review

(9 Publications)

Glycine Assay Kit (Fluorometric) (ab211100) provides a provides a simple and sensitive assay that detects physiological concentrations of glycine (GLY).

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

3 Images

Functional Studies - Glycine Assay Kit (Fluorometric) (AB211100)

FuncS

Supplier Data

Functional Studies - Glycine Assay Kit (Fluorometric) (AB211100)

Typical Glycine standard calibration curve.

FuncS

Supplier Data

Functional Studies - Glycine Assay Kit (Fluorometric) (AB211100)

Estimation of Glycine in human serum, saliva, and urine. Samples were deproteinized using >10 kD spin column (ab93349) and diluted with GLY Assay Buffer prior to performing assay : (serum 1 : 64 dilution; saliva 1 : 32 dilution; urine 1 : 128 dilution). 25 μL of each diluted sample was spiked with 0.3 nmol of Glycine Standard and assayed following the kit protocol. Glycine concentrations in serum (224 ± 21μM), saliva (149 ± 7 μM) and urine (54 ± 4 μM/mM creatinine).

Schematic Diagram - Glycine Assay Kit (Fluorometric) (AB211100)

Schematic Diagram

Supplier Data

Schematic Diagram - Glycine Assay Kit (Fluorometric) (AB211100)

Representative image of Glycine Assay Kit (Fluorometric) ab211100

Components shown from left to right :

- OxiRed Probe (2 vials)

- Converter Mix H

- Glycine Standard

- Developer Solution V

- Assay Buffer 58

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Fluorescent

Sample types

Tissue Lysate, Saliva, Urine, Plasma, Serum, Cell Lysate

Assay type

Quantitative

Sensitivity

= 1 µM

Assay Platform

Microplate reader

style

left-column

Product details

Glycine Assay Kit (Fluorometric) (ab211100) provides a provides a simple and sensitive assay that detects physiological concentrations of glycine (GLY).

Glycine assay principle
In the Glycine assay, Glycine is oxidized in the presence of GLY Enzyme Mix and is converted to an intermediate, which reacts with a fluorescent probe to generate a strong stable signal at Ex/Em = 535/587 nm. The intensity of the signal is directly proportional to the amount of GLY in the sample.

The reaction is specific and other amino acids do not interfere with the assay.

Glycine assay protocol summary
- Add samples and standards to wells
- Add reaction mix and incubate for 60 min min
- Analyze with fluorescence microplate reader (Ex/Em = 535/587 nm) in end point mode

Other Notes
This product was previously called K589 Biovision Glycine Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.

Glycine (GLY, G) is one of the 20 standard amino acids commonly found in proteins. Glycine's side chain is a hydrogen substituent, which makes it the smallest proteogenic and the only non-chiral amino acid. Basic functions of Glycine include the participation in the synthesis of creatine, glutathione, heme groups, and conjugated bile acids (bile salts). It is also present as one of the most abundant residues in the triple-helical structure of collagen.

Glycine acts as a glucogenic amino acid by regulating sugar levels in blood. Therefore, glycine supplementation has been used in patients suffering anemia, hypoglycemia and chronic fatigue. Glycine possesses both inhibitory and excitatory neurotransmitter functions in the brain stem and spinal cord. In cancer cells, glycine consumption is highly correlated to cancer cell proliferation via purine synthesis. Glycine uptake in cancer cell studies supports the role of this amino acid in tumorigenesis and malignancy.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

style

left-column

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab211100-100Test", "assetComponentDetails": [ { "size":"2 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB211100-CMP04", "productcode":"AB309437" }, { "size":"1 x 1 Vial", "name":"Glycine Standard", "number":"AB211100-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution V", "number":"AB211100-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Converter Mix H", "number":"AB211100-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 58", "number":"AB211100-CMP01", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycine a simple amino acid plays a critical role in various biological processes. It is often referred to as Gly or by its chemical formula C2H5NO2. Glycine has a molecular mass of approximately 75.07 g/mol. It is ubiquitously expressed in many tissues but is especially abundant in the central nervous system and connective tissues. Glycine acts as a building block for proteins and serves as an essential neurotransmitter in the central nervous system.

Biological function summary

Glycine influences the function of neurotransmitter systems. It serves as an inhibitory neurotransmitter in the spinal cord brainstem and retina through glycine receptors. Glycine forms part of collagen complexes in connective tissue contributing to structure and stability. Additionally glycine participates in the synthesis of other biomolecules like purines which are necessary for nucleic acid structure. Glycine's low molecular weight and small size enable it to fit into spaces within protein structures enhancing flexibility and function.

Pathways

Glycine influences the central nervous system's glycinergic pathways and is integral to the biosynthesis of serine and the glutathione pathway. Glycine's involvement in the glycinergic pathway associates it with glycine receptors and transporters that modulate synaptic transmissions. Within the glutathione pathway glycine works alongside glutamate and cysteine to form glutathione a vital antioxidant. This action highlights its cooperative role with proteins like the glutamate-cysteine ligase in cellular oxidative stress defense.

Glycine shows relevance to conditions such as glycine encephalopathy and schizophrenia. Excess glycine accumulation due to enzyme defects can lead to glycine encephalopathy impacting the central nervous system. Glycine concentrations and related receptor functions may also affect schizophrenia where neurotransmitter systems are disrupted. Glycine's involvement with NMDA receptors has been studied in these disorders suggesting its potential as a target for treatment strategies.

style

left-column

Product protocols

style

left-column

Target data

See full target information Glycine

style

left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 7:531-555 PubMed40033006

2025

Regulation of mammalian cellular metabolism by endogenous cyanide production.

Applications

Unspecified application

Species

Unspecified reactive species

Karim Zuhra,Maria Petrosino,Lucia Janickova,Jovan Petric,Kelly Ascenção,Thibaut Vignane,Moustafa Khalaf,Thilo M Philipp,Stella Ravani,Abhishek Anand,Vanessa Martins,Sidneia Santos,Serkan Erdemir,Sait Malkondu,Barbara Sitek,Taha Kelestemur,Anna Kieronska-Rudek,Tomas Majtan,Luis Filgueira,Darko Maric,Stefan Chlopicki,David Hoogewijs,György Haskó,Andreas Papapetropoulos,Brian A Logue,Gerry R Boss,Milos R Filipovic,Csaba Szabo

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2407134 PubMed39721015

2024

Fatty Acid Oxidation-Glycolysis Metabolic Transition Affects ECM Homeostasis in Silica-Induced Pulmonary Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqing Sun,Siyun Zhou,Lan Peng,Wei Wang,Yi Liu,Ting Wang,Demin Cheng,Ziwei Li,Haojie Xiong,Xinying Jia,Wenxiu Lian,Jiandong Jiao,Chunhui Ni

BMC cancer 24:1515 PubMed39696051

2024

Redox homeostasis of one-carbon metabolism-dependent reprogramming is critical for RCC progression under exogenous serine/glycine-deprived conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Huijuan Wang,Mengzhen Fan,Sichang Liu,Mengjiao Qu,Xin Hou,Junqing Hou,Yanxin Xu,Xiaodi Shang,Chen Liu,Mingxia He,Jianzheng Gao,Jingying Chen,Xia Li

International journal of biological sciences 20:4146-4161 PubMed39247829

2024

N6-methyladenosine modification of LATS2 promotes hepatoblastoma progression by inhibiting ferroptosis through the YAP1/ATF4/PSAT1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Guoqing Zhu,Yi Xie,Zhixuan Bian,Ji Ma,Ni Zhen,Tianshu Chen,Jiabei Zhu,Siwei Mao,Xiaochen Tang,Li Liu,Song Gu,Miao Ding,Qiuhui Pan

The Journal of clinical investigation 134: PubMed37962965

2024

Impaired glycine neurotransmission causes adolescent idiopathic scoliosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolu Wang,Ming Yue,Jason Pui Yin Cheung,Prudence Wing Hang Cheung,Yanhui Fan,Meicheng Wu,Xiaojun Wang,Sen Zhao,Anas M Khanshour,Jonathan J Rios,Zheyi Chen,Xiwei Wang,Wenwei Tu,Danny Chan,Qiuju Yuan,Dajiang Qin,Guixing Qiu,Zhihong Wu,Terry Jianguo Zhang,Shiro Ikegawa,Nan Wu,Carol A Wise,Yong Hu,Keith Dip Kei Luk,You-Qiang Song,Bo Gao

Nature communications 14:949 PubMed36810287

2023

Time-restricted feeding promotes muscle function through purine cycle and AMPK signaling in Drosophila obesity models.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Livelo,Yiming Guo,Farah Abou Daya,Vasanthi Rajasekaran,Shweta Varshney,Hiep D Le,Stephen Barnes,Satchidananda Panda,Girish C Melkani

Microorganisms 9: PubMed34207623

2021

Identifying a Novel Bile Salt Hydrolase from the Keystone Gut Bacterium .

Applications

Unspecified application

Species

Unspecified reactive species

Guillaume Déjean,Héloïse Tudela,Lisa Bruno,Déborah Kissi,Georges Rawadi,Sandrine P Claus

Cell reports 34:108869 PubMed33730581

2021

The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Michelle Grace Acoba,Ebru S Selen Alpergin,Santosh Renuse,Lucía Fernández-Del-Río,Ya-Wen Lu,Oleh Khalimonchuk,Catherine F Clarke,Akhilesh Pandey,Michael J Wolfgang,Steven M Claypool

Nature communications 7:13501 PubMed27874011

2016

Inhibition of glycine transporter-1 in the dorsal vagal complex improves metabolic homeostasis in diabetes and obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica T Y Yue,Mona A Abraham,Paige V Bauer,Mary P LaPierre,Peili Wang,Frank A Duca,Beatrice M Filippi,Owen Chan,Tony K T Lam

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Glycine Assay Kit (Fluorometric)

Functional Studies - Glycine Assay Kit (Fluorometric) (AB211100)

Functional Studies - Glycine Assay Kit (Fluorometric) (AB211100)

Schematic Diagram - Glycine Assay Kit (Fluorometric) (AB211100)

Key facts

Detection method

Sample types

Assay type

Sensitivity

Assay Platform

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Assay Kits

Product promise