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AB169558

Glycogen Assay Kit II (Colorimetric)

5

(1 Review)

|

(59 Publications)

Glycogen Assay Kit II (Colorimetric) ab169558 provides a simple, fast and robust way to measure glycogen levels in biological samples.

- Simple and fast protocol with results within one hour
- Can detect 4 - 40 µg/ml of glycogen in samples
- Cited in > 50 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
5 Images
Functional Studies - Glycogen Assay Kit II (Colorimetric) (AB169558)
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Functional Studies - Glycogen Assay Kit II (Colorimetric) (AB169558)

Glycogen measured in tissue lysates showing quantity (μg) per mg of extracted protein.

Protein concentration for samples varied from 25 mg/mL to 50 mg/mL. Samples were diluted 2-54 fold.

Functional Studies - Glycogen Assay Kit II (Colorimetric) (AB169558)
  • FuncS

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Functional Studies - Glycogen Assay Kit II (Colorimetric) (AB169558)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

Functional Studies - Glycogen Assay Kit II (Colorimetric) (AB169558)
  • FuncS

PubMed

Functional Studies - Glycogen Assay Kit II (Colorimetric) (AB169558)

Glycogen metabolism.

Glycogen level was highest in Alstonville larvae (n = 10 biological rep/mitotype).

Aw WC, Towarnicki SG, Melvin RG, et al., PLoS Genet. 2018;14(11):e1007735, Fig 14a, doi:10.1371/journal.pgen.1007735. Reproduced under the creative commons license. http://creativecommons.org/licenses/by/4.0/

Functional Studies - Glycogen Assay Kit II (Colorimetric) (AB169558)
  • FuncS

Unknown

Functional Studies - Glycogen Assay Kit II (Colorimetric) (AB169558)

Glycogen measured in cell lysates showing quantity (μg) per 1 mln cells.

Samples with the concentration of 1e7 cells/mL (HeLa) and 6.6e6 cells/mL (HCT116) were used. Samples were diluted 2-6 fold.

Schematic Diagram - Glycogen Assay Kit II (Colorimetric) (AB169558)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Glycogen Assay Kit II (Colorimetric) (AB169558)

Representative image of Glycogen Assay Kit II (Colorimetric) ab169558

Components shown from left to right :

- Glycogen Standard

- Developer Solution III

- Developer Mix D

- Hydrolysis Enzyme Mix I

- Assay Buffer 8

- Assay Buffer 2

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Quantitative

Sensitivity

> 4 µg/mL

Range

4 - 40 µg/mL

Assay time

1h

Assay Platform

Microplate reader

Product details

Glycogen Assay Kit II (Colorimetric) ab169558 provides a simple, fast and robust way to measure glycogen levels in biological samples. This kit uses a different readout chemistry to our most popular Glycogen Assay Kit ab65620.

How the assay works

In this glycogen assay protocol, glycogen is hydrolyzed into glucose using glucoamylase, glucose is then oxidized to form an intermediate that reduces a colorless probe to a colored product with strong absorbance at 450 nm.

Glycogen assay protocol summary:

- Add samples and standards to wells
- Add hydrolysis enzyme and incubate for 30 min at room temp
- Add reaction mix and incubate for 30 min at room temp
- Analyze with microplate reader

How other researchers are using Glycogen Assay Kit ab169558

Glycogen Assay Kit II has been used in a variety of sample types including:
Rat hepatic tissue homogenates 1
Human chondrosarcoma cells 2
Mouse adipose tissue samples 3
References:

1-Bzdega W et al. 2023
2-Pathmanapan S et al. 2023
3- Liebscher G et al. 2022

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab169558-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Hydrolysis Enzyme Mix I", "number":"AB169558-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"Glycogen Standard", "number":"AB169558-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix D", "number":"AB169558-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution III", "number":"AB169558-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 8", "number":"AB169558-CMP03", "productcode":"AB323941" }, { "size":"1 x 25 mL", "name":"Assay Buffer 2", "number":"AB169558-CMP02", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycogen acts as a critical energy storage polymer in the human body. It is a branched glucose polymer commonly referred to as animal starch which forms granular aggregates within cells. These aggregates typically weigh between 400 to 600 kDa. Glycogen is primarily expressed in the liver and skeletal muscle tissues. In the liver glycogen maintains blood glucose levels while in muscles it supplies energy during contraction. Measurement of glycogen levels in tissues can be performed using specialized tools like a glycogen assay kit glycogen x or a glycogen test.
Biological function summary

Glycogen functions to store energy in the form of glucose units allowing for a quick release when the body requires immediate energy. It does not belong to a traditional complex but does interact with various enzymes in glycogen metabolism. Glycogen phosphorylase and glycogen synthase regulate its breakdown and synthesis respectively. Enzymatic activities such as the hydrolysis of glycogen play a significant role in mobilizing glucose units from glycogen therefore facilitating metabolic processes.

Pathways

Glycogen is integral in glycolysis and gluconeogenesis. During glycolysis enzymes such as phosphoglucomutase convert glycogen-derived glucose-1-phosphate into glucose-6-phosphate which then enters glycolysis to produce energy. In gluconeogenesis glycogen can be synthesized as an energy reserve. Glycogen metabolism involves key proteins like insulin and glucagon that control glucose levels and consequently the activities of enzymes acting on glycogen.

Glycogen storage diseases (GSD) and type 2 diabetes illustrate the consequences of abnormal glycogen metabolism. The accumulation or deficiency of glycogen in tissues causes various forms of GSDs which lead to organ dysfunction. In diabetes impaired insulin action affects glycogen synthesis and breakdown resulting in dysregulated blood sugar levels. Key proteins associated with these conditions include insulin glucagon and glycogen synthase.

Product protocols

Publications (59)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eado3440 PubMed40378221

2025

is a metabolic switch that shifts hepatic energy storage from lipid to glycogen.

Applications

Unspecified application

Species

Unspecified reactive species

Kate Townsend Creasy,Minal B Mehta,Carolin V Schneider,Joseph Park,David Zhang,Swapnil V Shewale,John S Millar,Marijana Vujkovic,Nicholas J Hand,Paul M Titchenell,Joseph A Baur,Daniel J Rader

International journal of molecular sciences 26: PubMed40076869

2025

Hepatic Deletion of Carbohydrate Response Element Binding Protein Impairs Hepatocarcinogenesis in a High-Fat Diet-Induced Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Majedul Karim,Jessica Prey,Franziska Willer,Helen Leiner,Mohd Yasser,Frank Dombrowski,Silvia Ribback

Cell death discovery 10:486 PubMed39627194

2024

TNFα-CXCR1/2 partners in crime in insulin resistance conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Castelli,Housem Kacem,Laura Brandolini,Cristina Giorgio,Marta Sofia Scenna,Marcello Allegretti,Annamaria Cimini,Michele d'Angelo

Journal of cachexia, sarcopenia and muscle 15:2387-2401 PubMed39482890

2024

Short-chain fatty acids enhance muscle mass and function through the activation of mTOR signalling pathways in sarcopenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoran Liu,Pui Yan Wong,Qianjin Wang,Hei Yuet Wong,Tao Huang,Can Cui,Ning Zhang,Wing Hoi Cheung,Ronald Man Yeung Wong

Physiological reports 12:e70040 PubMed39256891

2024

PKM2 regulates metabolic flux and oxidative stress in the murine heart.

Applications

Unspecified application

Species

Unspecified reactive species

Katie C Y Lee,Allison L Williams,Lu Wang,Guoxiang Xie,Wei Jia,Anastasia Fujimoto,Mariana Gerschenson,Ralph V Shohet

Nutrients 16: PubMed39064713

2024

Impaired Fat Absorption from Intestinal Tract in High-Fat Diet Fed Male Mice Deficient in Proglucagon-Derived Peptides.

Applications

Unspecified application

Species

Unspecified reactive species

Koki Nishida,Shinji Ueno,Yusuke Seino,Shihomi Hidaka,Naoya Murao,Yuki Asano,Haruki Fujisawa,Megumi Shibata,Takeshi Takayanagi,Kento Ohbayashi,Yusaku Iwasaki,Katsumi Iizuka,Shoei Okuda,Mamoru Tanaka,Tadashi Fujii,Takumi Tochio,Daisuke Yabe,Yuuichiro Yamada,Yoshihisa Sugimura,Yoshiki Hirooka,Yoshitaka Hayashi,Atsushi Suzuki

The Journal of biological chemistry 300:107569 PubMed39009342

2024

Glycogen myophosphorylase loss causes increased dependence on glucose in iPSC-derived retinal pigment epithelium.

Applications

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Species

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Basudha Basu,Magdalena Karwatka,Becky China,Martin McKibbin,Kamron Khan,Chris F Inglehearn,John E Ladbury,Colin A Johnson

Nutrients 16: PubMed38931176

2024

The Metabolic and Endocrine Effects of a 12-Week Allulose-Rich Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin B Cayabyab,Marley J Shin,Micah S Heimuli,Iris J Kim,Dominic P D'Agostino,Richard J Johnson,Andrew P Koutnik,Nick Bellissimo,David M Diamond,Nicholas G Norwitz,Juan A Arroyo,Paul R Reynolds,Benjamin T Bikman

Molecular neurodegeneration 19:50 PubMed38902734

2024

Mis-localization of endogenous TDP-43 leads to ALS-like early-stage metabolic dysfunction and progressive motor deficits.

Applications

Unspecified application

Species

Unspecified reactive species

Yiying Hu,Alexander Hruscha,Chenchen Pan,Martina Schifferer,Michael K Schmidt,Brigitte Nuscher,Martin Giera,Sarantos Kostidis,Özge Burhan,Frauke van Bebber,Dieter Edbauer,Thomas Arzberger,Christian Haass,Bettina Schmid

Redox biology 74:103234 PubMed38861834

2024

Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyun Guo,Yumeng Li,Shiquan Wang,Yongheng Yang,Tiantian Xu,Jianshuai Zhao,Jin Wang,Wenqiang Zuo,Pengju Wang,Guangchao Zhao,Huaning Wang,Wugang Hou,Hailong Dong,Yanhui Cai
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