Glycogen Assay Kit II (Colorimetric)
5
(1 Review)
|
(59 Publications)
- Simple and fast protocol with results within one hour
- Can detect 4 - 40 µg/ml of glycogen in samples
- Cited in > 50 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
- FuncS
Unknown
Functional Studies - Glycogen Assay Kit II (Colorimetric) (AB169558)
Glycogen measured in tissue lysates showing quantity (μg) per mg of extracted protein.
Protein concentration for samples varied from 25 mg/mL to 50 mg/mL. Samples were diluted 2-54 fold.
- FuncS
Unknown
Functional Studies - Glycogen Assay Kit II (Colorimetric) (AB169558)
Standard curve : mean of duplicates (+/- SD) with background reads subtracted
- FuncS
PubMed
Functional Studies - Glycogen Assay Kit II (Colorimetric) (AB169558)
Glycogen metabolism.
Glycogen level was highest in Alstonville larvae (n = 10 biological rep/mitotype).
Aw WC, Towarnicki SG, Melvin RG, et al., PLoS Genet. 2018;14(11):e1007735, Fig 14a, doi:10.1371/journal.pgen.1007735. Reproduced under the creative commons license. http://creativecommons.org/licenses/by/4.0/
- FuncS
Unknown
Functional Studies - Glycogen Assay Kit II (Colorimetric) (AB169558)
Glycogen measured in cell lysates showing quantity (μg) per 1 mln cells.
Samples with the concentration of 1e7 cells/mL (HeLa) and 6.6e6 cells/mL (HCT116) were used. Samples were diluted 2-6 fold.
- Schematic Diagram
Supplier Data
Schematic Diagram - Glycogen Assay Kit II (Colorimetric) (AB169558)
Representative image of Glycogen Assay Kit II (Colorimetric) ab169558
Components shown from left to right :
- Glycogen Standard
- Developer Solution III
- Developer Mix D
- Hydrolysis Enzyme Mix I
- Assay Buffer 8
- Assay Buffer 2
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
Product details
Glycogen Assay Kit II (Colorimetric) ab169558 provides a simple, fast and robust way to measure glycogen levels in biological samples. This kit uses a different readout chemistry to our most popular Glycogen Assay Kit ab65620.
How the assay works
In this glycogen assay protocol, glycogen is hydrolyzed into glucose using glucoamylase, glucose is then oxidized to form an intermediate that reduces a colorless probe to a colored product with strong absorbance at 450 nm.
Glycogen assay protocol summary:
- Add samples and standards to wells
- Add hydrolysis enzyme and incubate for 30 min at room temp
- Add reaction mix and incubate for 30 min at room temp
- Analyze with microplate reader
How other researchers are using Glycogen Assay Kit ab169558
Glycogen Assay Kit II has been used in a variety of sample types including:
Rat hepatic tissue homogenates 1
Human chondrosarcoma cells 2
Mouse adipose tissue samples 3
References:
1-Bzdega W et al. 2023
2-Pathmanapan S et al. 2023
3- Liebscher G et al. 2022
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Glycogen functions to store energy in the form of glucose units allowing for a quick release when the body requires immediate energy. It does not belong to a traditional complex but does interact with various enzymes in glycogen metabolism. Glycogen phosphorylase and glycogen synthase regulate its breakdown and synthesis respectively. Enzymatic activities such as the hydrolysis of glycogen play a significant role in mobilizing glucose units from glycogen therefore facilitating metabolic processes.
Pathways
Glycogen is integral in glycolysis and gluconeogenesis. During glycolysis enzymes such as phosphoglucomutase convert glycogen-derived glucose-1-phosphate into glucose-6-phosphate which then enters glycolysis to produce energy. In gluconeogenesis glycogen can be synthesized as an energy reserve. Glycogen metabolism involves key proteins like insulin and glucagon that control glucose levels and consequently the activities of enzymes acting on glycogen.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (59)
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Science advances 11:eado3440 PubMed40378221
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Physiological reports 12:e70040 PubMed39256891
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Nutrients 16: PubMed39064713
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The Journal of biological chemistry 300:107569 PubMed39009342
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Nutrients 16: PubMed38931176
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Molecular neurodegeneration 19:50 PubMed38902734
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Redox biology 74:103234 PubMed38861834
2024
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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