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Glycogen Assay Kit II (Colorimetric) ab169558 provides a simple, fast and robust way to measure glycogen levels in biological samples.

- Simple and fast protocol with results within one hour
- Can detect 4 - 40 µg/ml of glycogen in samples
- Cited in > 50 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.


Images

Functional Studies - Glycogen Assay Kit II (Colorimetric) (AB169558), expandable thumbnail
  • Functional Studies - Glycogen Assay Kit II (Colorimetric) (AB169558), expandable thumbnail
  • Functional Studies - Glycogen Assay Kit II (Colorimetric) (AB169558), expandable thumbnail
  • Functional Studies - Glycogen Assay Kit II (Colorimetric) (AB169558), expandable thumbnail

Publications

Key facts

Detection method
Colorimetric
Sample types
Tissue, Suspension cells, Adherent cells
Assay type
Quantitative
Reactive species
Mammals
Range
4 - 40 µg/mL
Assay time
1h
Sensitivity
> 4 µg/mL

Associated Products

Select an associated product type

1 product for Alternative Product

What's included?

100 Test
Components
Assay Buffer 2
1 x 25 mL
Assay Buffer 8
1 x 25 mL
Developer Mix D
1 x 1 Vial
Developer Solution III
1 x 1 Vial
Glycogen Standard
1 x 100 µL
Hydrolysis Enzyme Mix I
1 x 1 Vial

Recommended products

Glycogen Assay Kit II (Colorimetric) ab169558 provides a simple, fast and robust way to measure glycogen levels in biological samples.

- Simple and fast protocol with results within one hour
- Can detect 4 - 40 µg/ml of glycogen in samples
- Cited in > 50 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Detection method
Colorimetric
Sample types
Tissue, Suspension cells, Adherent cells
Assay type
Quantitative
Reactive species
Mammals
Range
4 - 40 µg/mL
Assay time
1h
Assay Platform
Microplate reader
Sensitivity
> 4 µg/mL

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Glycogen Assay Kit II (Colorimetric) ab169558 provides a simple, fast and robust way to measure glycogen levels in biological samples. This kit uses a different readout chemistry to our most popular Glycogen Assay Kit Glycogen Assay Kit ab65620.

How the assay works

In this glycogen assay protocol, glycogen is hydrolyzed into glucose using glucoamylase, glucose is then oxidized to form an intermediate that reduces a colorless probe to a colored product with strong absorbance at 450 nm.

Glycogen assay protocol summary:

- Add samples and standards to wells
- Add hydrolysis enzyme and incubate for 30 min at room temp
- Add reaction mix and incubate for 30 min at room temp
- Analyze with microplate reader

How other researchers are using Glycogen Assay Kit ab169558

Glycogen Assay Kit II has been used in a variety of sample types including:
Rat hepatic tissue homogenates 1
Human chondrosarcoma cells 2
Mouse adipose tissue samples 3
References: 

1-Bzdega W et al. 2023
2-Pathmanapan S et al. 2023
3- Liebscher G et al. 2022

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Glycogen acts as a critical energy storage polymer in the human body. It is a branched glucose polymer commonly referred to as animal starch which forms granular aggregates within cells. These aggregates typically weigh between 400 to 600 kDa. Glycogen is primarily expressed in the liver and skeletal muscle tissues. In the liver glycogen maintains blood glucose levels while in muscles it supplies energy during contraction. Measurement of glycogen levels in tissues can be performed using specialized tools like a glycogen assay kit glycogen x or a glycogen test.

Biological function summary

Glycogen functions to store energy in the form of glucose units allowing for a quick release when the body requires immediate energy. It does not belong to a traditional complex but does interact with various enzymes in glycogen metabolism. Glycogen phosphorylase and glycogen synthase regulate its breakdown and synthesis respectively. Enzymatic activities such as the hydrolysis of glycogen play a significant role in mobilizing glucose units from glycogen therefore facilitating metabolic processes.

Pathways

Glycogen is integral in glycolysis and gluconeogenesis. During glycolysis enzymes such as phosphoglucomutase convert glycogen-derived glucose-1-phosphate into glucose-6-phosphate which then enters glycolysis to produce energy. In gluconeogenesis glycogen can be synthesized as an energy reserve. Glycogen metabolism involves key proteins like insulin and glucagon that control glucose levels and consequently the activities of enzymes acting on glycogen.

Associated diseases and disorders

Glycogen storage diseases (GSD) and type 2 diabetes illustrate the consequences of abnormal glycogen metabolism. The accumulation or deficiency of glycogen in tissues causes various forms of GSDs which lead to organ dysfunction. In diabetes impaired insulin action affects glycogen synthesis and breakdown resulting in dysregulated blood sugar levels. Key proteins associated with these conditions include insulin glucagon and glycogen synthase.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Functional Studies - Glycogen Assay Kit II (Colorimetric) (ab169558), expandable thumbnail

    Functional Studies - Glycogen Assay Kit II (Colorimetric) (ab169558)

    Glycogen measured in tissue lysates showing quantity (μg) per mg of extracted protein.

    Protein concentration for samples varied from 25 mg/mL to 50 mg/mL. Samples were diluted 2-54 fold.

  • Functional Studies - Glycogen Assay Kit II (Colorimetric) (ab169558), expandable thumbnail

    Functional Studies - Glycogen Assay Kit II (Colorimetric) (ab169558)

    Glycogen measured in cell lysates showing quantity (μg) per 1 mln cells.

    Samples with the concentration of 1e7 cells/mL (HeLa) and 6.6e6 cells/mL (HCT116) were used. Samples were diluted 2-6 fold.

  • Functional Studies - Glycogen Assay Kit II (Colorimetric) (ab169558), expandable thumbnail

    Functional Studies - Glycogen Assay Kit II (Colorimetric) (ab169558)

    Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Functional Studies - Glycogen Assay Kit II (Colorimetric) (ab169558), expandable thumbnail
    Aw WC, Towarnicki SG, Melvin RG, et al., PLoS Genet. 2018;14(11):e1007735, Fig 14a, doi:10.1371/journal.pgen.1007735. Reproduced under the creative commons license. http://creativecommons.org/licenses/by/4.0/

    Functional Studies - Glycogen Assay Kit II (Colorimetric) (ab169558)

    Glycogen metabolism.

    Glycogen level was highest in Alstonville larvae (n = 10 biological rep/mitotype).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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