Glycogen Assay Kit II (Colorimetric) ab169558 provides a simple, fast and robust way to measure glycogen levels in biological samples.
- Simple and fast protocol with results within one hour
- Can detect 4 - 40 µg/ml of glycogen in samples
- Cited in > 50 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Nutrients 15:2023How Does CBG Administration Affect Sphingolipid Deposition in the Liver of Insulin-Resistant Rats?Applications:Unspecified applicationReactive species:Unspecified reactive speciesWiktor Bzdęga,Piotr Franciszek Kurzyna,Ewa Harasim-Symbor,Adam Hołownia,Adrian Chabowski,Karolina Konstantynowicz-NowickaPubMed 37892425
- Cell reports 42:1125782023Mutant IDH regulates glycogen metabolism from early cartilage development to malignant chondrosarcoma formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSinthu Pathmanapan,Raymond Poon,Tomasa Barrientos De Renshaw,Puviindran Nadesan,Makoto Nakagawa,Gireesh A Seesankar,Adrian Kwan Ho Loe,Hongyuan H Zhang,Joan J Guinovart,Jordi Duran,Christopher B Newgard,Jay S Wunder,Benjamin A AlmanPubMed 37267108
- Molecular metabolism 71:1017052023The lysosomal LAMTOR / Ragulator complex is essential for nutrient homeostasis in brown adipose tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGudrun Liebscher,Nemanja Vujic,Renate Schreiber,Markus Heine,Caroline Krebiehl,Madalina Duta-Mare,Giorgia Lamberti,Cedric H de Smet,Michael W Hess,Thomas O Eichmann,Sarah Hölzl,Ludger Scheja,Joerg Heeren,Dagmar Kratky,Lukas A HuberPubMed 36907508
- iScience 26:1059112023Perturbation of placental protein glycosylation by endoplasmic reticulum stress promotes maladaptation of maternal hepatic glucose metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong Wa Yung,Xiaohui Zhao,Luke Glover,Charlotte Burrin,Poh-Choo Pang,Carolyn J P Jones,Carolyn Gill,Kate Duhig,Matts Olovsson,Lucy C Chappell,Stuart M Haslam,Anne Dell,Graham J Burton,D Stephen Charnock-JonesPubMed 36660474
- Toxics 11:2023Can Maternal Exposure to Air Pollution Affect Post-Natal Liver Development?Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Song,Ling Chen,Ellen Bennett,Amanda J Wheeler,Katherine Southam,Seiha Yen,Fay Johnston,Graeme R ZoskyPubMed 36668787
- Nature communications 13:70382022N6-methyladenosine modification governs liver glycogenesis by stabilizing the glycogen synthase 2 mRNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Zhang,Huilong Yin,Xiaofang Zhang,Xunliang Jiang,Yongkang Liu,Haolin Zhang,Yingran Peng,Da Li,Yanping Yu,Jinbao Zhang,Shuli Cheng,Angang Yang,Rui ZhangPubMed 36396934
- Insect science 30:816-8282022Functional evaluation of the insulin/insulin-like growth factor signaling pathway in determination of wing polyphenism in pea aphid.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiyang Yuan,Yanyan Wang,Wanwan Ye,Erliang Yuan,Jian Di,Xin Chen,Yanling Xing,Yucheng Sun,Feng GePubMed 36178731
- Frontiers in immunology 13:9766282022Enforcing GLUT3 expression in CD8 T cells improves fitness and tumor control by promoting glucose uptake and energy storage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisabetta Cribioli,Greta Maria Paola Giordano Attianese,Pierpaolo Ginefra,Amandine Signorino-Gelo,Romain Vuillefroy de Silly,Nicola Vannini,Christoph Hess,Melita Irving,George CoukosPubMed 36203587
- Molecular biology of the cell 33:ar1062022Dynamic glucose uptake, storage, and release by human microvascular endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamaneh Yazdani,Philip J Bilan,Javier R Jaldin-Fincati,Janice Pang,Felicia Ceban,Ekambir Saran,John H Brumell,Spencer A Freeman,Amira KlipPubMed 35921166
- PloS one 17:e02696202022Evidence for a novel, effective approach to targeting carcinoma catabolism exploiting the first-in-class, anti-cancer mitochondrial drug, CPI-613.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMoises O Guardado Rivas,Shawn D Stuart,Daniel Thach,Michael Dahan,Robert Shorr,Zuzana Zachar,Paul M BinghamPubMed 35675354
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Mammals
- Range
- 4 - 40 µg/mL
- Assay time
- 1h
- Sensitivity
- > 4 µg/mL
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Glycogen Assay Kit II (Colorimetric) ab169558 provides a simple, fast and robust way to measure glycogen levels in biological samples.
- Simple and fast protocol with results within one hour
- Can detect 4 - 40 µg/ml of glycogen in samples
- Cited in > 50 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Mammals
- Range
- 4 - 40 µg/mL
- Assay time
- 1h
- Assay Platform
- Microplate reader
- Sensitivity
- > 4 µg/mL
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Glycogen Assay Kit II (Colorimetric) ab169558 provides a simple, fast and robust way to measure glycogen levels in biological samples. This kit uses a different readout chemistry to our most popular Glycogen Assay Kit Glycogen Assay Kit ab65620.
How the assay works
In this glycogen assay protocol, glycogen is hydrolyzed into glucose using glucoamylase, glucose is then oxidized to form an intermediate that reduces a colorless probe to a colored product with strong absorbance at 450 nm.
Glycogen assay protocol summary:
- Add samples and standards to wells
- Add hydrolysis enzyme and incubate for 30 min at room temp
- Add reaction mix and incubate for 30 min at room temp
- Analyze with microplate reader
How other researchers are using Glycogen Assay Kit ab169558
Glycogen Assay Kit II has been used in a variety of sample types including:
Rat hepatic tissue homogenates 1
Human chondrosarcoma cells 2
Mouse adipose tissue samples 3
References:
1-Bzdega W et al. 2023
2-Pathmanapan S et al. 2023
3- Liebscher G et al. 2022
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glycogen acts as a critical energy storage polymer in the human body. It is a branched glucose polymer commonly referred to as animal starch which forms granular aggregates within cells. These aggregates typically weigh between 400 to 600 kDa. Glycogen is primarily expressed in the liver and skeletal muscle tissues. In the liver glycogen maintains blood glucose levels while in muscles it supplies energy during contraction. Measurement of glycogen levels in tissues can be performed using specialized tools like a glycogen assay kit glycogen x or a glycogen test.
- Biological function summary
Glycogen functions to store energy in the form of glucose units allowing for a quick release when the body requires immediate energy. It does not belong to a traditional complex but does interact with various enzymes in glycogen metabolism. Glycogen phosphorylase and glycogen synthase regulate its breakdown and synthesis respectively. Enzymatic activities such as the hydrolysis of glycogen play a significant role in mobilizing glucose units from glycogen therefore facilitating metabolic processes.
- Pathways
Glycogen is integral in glycolysis and gluconeogenesis. During glycolysis enzymes such as phosphoglucomutase convert glycogen-derived glucose-1-phosphate into glucose-6-phosphate which then enters glycolysis to produce energy. In gluconeogenesis glycogen can be synthesized as an energy reserve. Glycogen metabolism involves key proteins like insulin and glucagon that control glucose levels and consequently the activities of enzymes acting on glycogen.
- Associated diseases and disorders
Glycogen storage diseases (GSD) and type 2 diabetes illustrate the consequences of abnormal glycogen metabolism. The accumulation or deficiency of glycogen in tissues causes various forms of GSDs which lead to organ dysfunction. In diabetes impaired insulin action affects glycogen synthesis and breakdown resulting in dysregulated blood sugar levels. Key proteins associated with these conditions include insulin glucagon and glycogen synthase.
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4 product images
Functional Studies - Glycogen Assay Kit II (Colorimetric) (ab169558)
Glycogen measured in tissue lysates showing quantity (μg) per mg of extracted protein.
Protein concentration for samples varied from 25 mg/mL to 50 mg/mL. Samples were diluted 2-54 fold.
Functional Studies - Glycogen Assay Kit II (Colorimetric) (ab169558)
Glycogen measured in cell lysates showing quantity (μg) per 1 mln cells.
Samples with the concentration of 1e7 cells/mL (HeLa) and 6.6e6 cells/mL (HCT116) were used. Samples were diluted 2-6 fold.
Functional Studies - Glycogen Assay Kit II (Colorimetric) (ab169558)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Aw WC, Towarnicki SG, Melvin RG, et al., PLoS Genet. 2018;14(11):e1007735, Fig 14a, doi:10.1371/journal.pgen.1007735. Reproduced under the creative commons license. http://creativecommons.org/licenses/by/4.0/Functional Studies - Glycogen Assay Kit II (Colorimetric) (ab169558)
Glycogen metabolism.
Glycogen level was highest in Alstonville larvae (n = 10 biological rep/mitotype).
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