Glycogen Colorimetric Assay Kit
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(2 Publications)
- FuncS
Unknown
Functional Studies - Glycogen Colorimetric Assay Kit (AB282931)
Glycogen in rat liver lysate.
Rat liver (17 mg) was homogenized with 680 μl of deionized water, boiled for 10 min, and then centrifuged for 10 min. at 18000 x g. Supernatant was collected. Supernatant was diluted 10 times and 5 μl was used for the assay following the kit's protocol. Sample was spiked with 0.2 μg of Glycogen Standard.
- FuncS
Unknown
Functional Studies - Glycogen Colorimetric Assay Kit (AB282931)
Glycogen Standard Curve
Product details
This Glycogen Colorimetric Assay Kit (ab282931) is an easy and convenient assay to measure glycogen levels in biological samples. In the assay, glucoamylase hydrolyzes the glycogen releasing glucose which is then specifically oxidized to produce a product that reacts with OxiRed Probe to generate color (OD 590 nm). The method is quantitative, rapid, simple, sensitive, and designed for high throughput format. The assay can detect as low as 0.025 μg of glycogen in 384-well assay format.
Sample Type: Animal tissues such as liver etc and Cell culture: adherent or suspension cells
This product is manufactured by BioVision, an Abcam company and was previously called K960 EZScreen™ Glycogen Colorimetric Assay Kit (384-Well). K960-400 is the same size as the 400 test size of ab282931.
Glycogen is a branched polysaccharide, made of glucose units that serves as the primary source of energy storage in animals. Most of the glucose residues in glycogen are linked by α-1,4-glycosidic bonds with branching via α-1,6 linkage. In mammals, the two major sites of glycogen storage are liver and skeletal muscle. In liver, glycogen synthesis and degradation are regulated to maintain blood-glucose levels, while stored glycogen in muscle can be processed to meet the energy needs of the muscle itself. Abnormal ability to utilize glycogen is found in patients suffering diabetes and other genetic glycogen storage related diseases.
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REACH authorisation
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Properties and storage information
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Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Glycogen functions to store energy in the form of glucose units allowing for a quick release when the body requires immediate energy. It does not belong to a traditional complex but does interact with various enzymes in glycogen metabolism. Glycogen phosphorylase and glycogen synthase regulate its breakdown and synthesis respectively. Enzymatic activities such as the hydrolysis of glycogen play a significant role in mobilizing glucose units from glycogen therefore facilitating metabolic processes.
Pathways
Glycogen is integral in glycolysis and gluconeogenesis. During glycolysis enzymes such as phosphoglucomutase convert glycogen-derived glucose-1-phosphate into glucose-6-phosphate which then enters glycolysis to produce energy. In gluconeogenesis glycogen can be synthesized as an energy reserve. Glycogen metabolism involves key proteins like insulin and glucagon that control glucose levels and consequently the activities of enzymes acting on glycogen.
Product protocols
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Publications (2)
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Journal of xenobiotics 15: PubMed39846548
2025
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Pharmaceutics 15: PubMed37765275
2023
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