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AB282931

Glycogen Colorimetric Assay Kit

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(2 Publications)

Glycogen Colorimetric Assay Kit ab282931 is an easy and convenient assay to measure glycogen levels in biological samples.
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Functional Studies - Glycogen Colorimetric Assay Kit (AB282931)
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Functional Studies - Glycogen Colorimetric Assay Kit (AB282931)

Glycogen in rat liver lysate.

Rat liver (17 mg) was homogenized with 680 μl of deionized water, boiled for 10 min, and then centrifuged for 10 min. at 18000 x g. Supernatant was collected. Supernatant was diluted 10 times and 5 μl was used for the assay following the kit's protocol. Sample was spiked with 0.2 μg of Glycogen Standard.

Functional Studies - Glycogen Colorimetric Assay Kit (AB282931)
  • FuncS

Unknown

Functional Studies - Glycogen Colorimetric Assay Kit (AB282931)

Glycogen Standard Curve

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Results type

Quantitative

Sensitivity

= 0.025 µg

Product details

This Glycogen Colorimetric Assay Kit (ab282931) is an easy and convenient assay to measure glycogen levels in biological samples. In the assay, glucoamylase hydrolyzes the glycogen releasing glucose which is then specifically oxidized to produce a product that reacts with OxiRed Probe to generate color (OD 590 nm). The method is quantitative, rapid, simple, sensitive, and designed for high throughput format. The assay can detect as low as 0.025 μg of glycogen in 384-well assay format.

Sample Type: Animal tissues such as liver etc and Cell culture: adherent or suspension cells

This product is manufactured by BioVision, an Abcam company and was previously called K960 EZScreen™ Glycogen Colorimetric Assay Kit (384-Well). K960-400 is the same size as the 400 test size of ab282931.

Glycogen is a branched polysaccharide, made of glucose units that serves as the primary source of energy storage in animals. Most of the glucose residues in glycogen are linked by α-1,4-glycosidic bonds with branching via α-1,6 linkage. In mammals, the two major sites of glycogen storage are liver and skeletal muscle. In liver, glycogen synthesis and degradation are regulated to maintain blood-glucose levels, while stored glycogen in muscle can be processed to meet the energy needs of the muscle itself. Abnormal ability to utilize glycogen is found in patients suffering diabetes and other genetic glycogen storage related diseases.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

What's included?

{ "values": { "400Test": { "sellingSize": "400 Test", "publicAssetCode":"ab282931-400Test", "assetComponentDetails": [ { "size":"4 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB282931-CMP06", "productcode":"AB309437" }, { "size":"1 x 100 µL", "name":"Glycogen Standard", "number":"AB282931-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix B", "number":"AB282931-CMP04", "productcode":"AB154151" }, { "size":"1 x 25 mL", "name":"Assay Buffer 2", "number":"AB282931-CMP03", "productcode":"AB136652" }, { "size":"1 x 1 Vial", "name":"Hydrolysis Enzyme Mix I", "number":"AB282931-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 8", "number":"AB282931-CMP01", "productcode":"AB323941" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycogen acts as a critical energy storage polymer in the human body. It is a branched glucose polymer commonly referred to as animal starch which forms granular aggregates within cells. These aggregates typically weigh between 400 to 600 kDa. Glycogen is primarily expressed in the liver and skeletal muscle tissues. In the liver glycogen maintains blood glucose levels while in muscles it supplies energy during contraction. Measurement of glycogen levels in tissues can be performed using specialized tools like a glycogen assay kit glycogen x or a glycogen test.
Biological function summary

Glycogen functions to store energy in the form of glucose units allowing for a quick release when the body requires immediate energy. It does not belong to a traditional complex but does interact with various enzymes in glycogen metabolism. Glycogen phosphorylase and glycogen synthase regulate its breakdown and synthesis respectively. Enzymatic activities such as the hydrolysis of glycogen play a significant role in mobilizing glucose units from glycogen therefore facilitating metabolic processes.

Pathways

Glycogen is integral in glycolysis and gluconeogenesis. During glycolysis enzymes such as phosphoglucomutase convert glycogen-derived glucose-1-phosphate into glucose-6-phosphate which then enters glycolysis to produce energy. In gluconeogenesis glycogen can be synthesized as an energy reserve. Glycogen metabolism involves key proteins like insulin and glucagon that control glucose levels and consequently the activities of enzymes acting on glycogen.

Glycogen storage diseases (GSD) and type 2 diabetes illustrate the consequences of abnormal glycogen metabolism. The accumulation or deficiency of glycogen in tissues causes various forms of GSDs which lead to organ dysfunction. In diabetes impaired insulin action affects glycogen synthesis and breakdown resulting in dysregulated blood sugar levels. Key proteins associated with these conditions include insulin glucagon and glycogen synthase.

Product protocols

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of xenobiotics 15: PubMed39846548

2025

Antidiabetic Effects of Quercetin and Silk Sericin in Attenuating Dysregulation of Hepatic Gluconeogenesis in Diabetic Rats Through Potential Modulation of PI3K/Akt/FOXO1 Signaling: In Vivo and In Silico Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Heba M Abdou,Ghada M Abd Elmageed,Hussein K Hussein,Imane Yamari,Samir Chtita,Lamia M El-Samad,Mohamed A Hassan

Pharmaceutics 15: PubMed37765275

2023

The Synergistic Influence of Polyflavonoids from on Diabetes Treatment and Their Modulation of the PI3K/AKT/FOXO1 Signaling Pathways: Molecular Docking Analyses and In Vivo Investigations.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed A Hassan,Ghada M Abd Elmageed,Ibtehal G El-Qazaz,Doaa S El-Sayed,Lamia M El-Samad,Heba M Abdou
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