Glycogen Phosphorylase Activity Assay Kit (Fluorometric)

Product details

This product is manufactured by BioVision, an Abcam company and was previously sold under product code K2104.

Glycogen phosphorylase (EC 2.4.1.1) catalyzes the rate-limiting step in the glycogenolysis pathway, cleaving the α1-4 glycoside bonds on linear glycogen chains to release glucose and utilizing inorganic phosphate to produce glucose-1-phosphate (G1P). In mammals, glycogen phosphorylase is abundant in muscle, liver and brain tissues. There are two forms of glycogen phosphorylase, namely glycogen phosphorylase a and b isoforms. Glycogen phosphorylase a is the highly active form found in muscle tissue, whereas glycogen phosphorylase b isoform has only limited activity. Glycogen phosphorylase is a clinically significant enzyme, as various mutations are associated with different congenital glycogen storage diseases affecting both muscle and liver. In addition, this enzyme has been suggested as a biomarker for gastric cancer and has been investigated as a drug target for treatment of type 2 diabetes. The Glycogen Phosphorylase Activity Assay Kit is based on the detection of glucose-1-phosphate by a set of enzymatic reactions, ultimately reducing a fluorogenic probe to form a stable fluorophore measured at Ex/Em = 538/587 nm. The intensity of the fluorescence is directly proportional to the glycogen phosphorylase activity in samples. This kit is a rapid, sensitive and a convenient tool for detecting glycogen phosphorylase activity in samples. The kit can detect as low as 30 μU of enzyme activity in a variety of sample types.