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AB273271

Glycogen Phosphorylase Assay Kit (Colorimetric)

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(4 Publications)

Glycogen Phosphorylase Assay Kit ab273271 is based on the detection of G1P by a set of enzymatic reactions to generate a colored product with a strong absorbance at 450 nm.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
4 Images
Functional Studies - Glycogen Phosphorylase Assay Kit (Colorimetric) (AB273271)
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Functional Studies - Glycogen Phosphorylase Assay Kit (Colorimetric) (AB273271)

Glycogen phosphorylase activity in different sample types.

Specific activity of Glycogen Phosphorylase in different sample types.

Functional Studies - Glycogen Phosphorylase Assay Kit (Colorimetric) (AB273271)
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Supplier Data

Functional Studies - Glycogen Phosphorylase Assay Kit (Colorimetric) (AB273271)

Reaction time-course.

Reaction curves of Glycogen Phosphorylase versus no enzyme control.

Functional Studies - Glycogen Phosphorylase Assay Kit (Colorimetric) (AB273271)
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Supplier Data

Functional Studies - Glycogen Phosphorylase Assay Kit (Colorimetric) (AB273271)

G1P Standard Curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

G1P Standard Curve.

Schematic Diagram - Glycogen Phosphorylase Assay Kit (Colorimetric) (AB273271)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Glycogen Phosphorylase Assay Kit (Colorimetric) (AB273271)

Representative image of Glycogen Phosphorylase Assay Kit (Colorimetric) ab273271

Components shown from left to right :

- Developer Solution III

- Enzyme Mix XVII

- G1P Standard

- Glycogen Phosphorylase

- Developer Mix G

- Glycogen Substrate

- Assay Buffer 37

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Adherent cells, Suspension cells, Tissue

Assay type

Enzyme activity (quantitative)

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Glycogen Phosphorylase Assay Kit (Colorimetric) (ab273271) is based on the detection of G1P by a set of enzymatic reactions to generate a colored product with a strong absorbance at 450 nm. The OD 450 nm signal is directly proportional to the glycogen phosphorylase activity. The Glycogen Phosphorylase Colorimetric Assay Kit is rapid, sensitive and convenient tool for detecting glycogen phosphorylase activity. The kit can detect as low as 10 mU in a variety of sample types.

Other Notes
This product was previously called K179 Biovision Glycogen Phosphorylase Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273271-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Glycogen Phosphorylase", "number":"AB273271-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"G1P Standard", "number":"AB273271-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Enzyme Mix XVII", "number":"AB273271-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"Assay Buffer 37", "number":"AB273271-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix G", "number":"AB273271-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution III", "number":"AB273271-CMP07", "productcode":"" }, { "size":"1 x 1 Each", "name":"Glycogen Substrate", "number":"AB273271-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycogen Phosphorylase also known as 'Phosphorylase' is an enzyme that catalyzes the breakdown of glycogen into glucose-1-phosphate. This enzyme exists in different isoforms including muscle liver and brain glycogen phosphorylases. The molecular mass of glycogen phosphorylase is approximately 97 kDa. It typically expresses in tissues that store glycogen such as liver and muscle enabling these tissues to convert stored glycogen into glucose during times of energy demand.
Biological function summary

This protein plays a role in glycogen metabolism by releasing glucose units through the glycogen phosphorylase reaction. It is not part of a complex but functions independently. Glycogen phosphorylase activity increases during fasting or physical exertion providing an accessible source of glucose. This regulation is important for maintaining blood glucose levels especially in liver tissue where glycogen phosphorylase function is essential for homeostasis.

Pathways

Glycogen phosphorylase is integral to glycogenolysis a part of carbohydrate metabolism. It works alongside other enzymes such as glycogen synthase in balancing the breakdown and synthesis of glycogen. In hormonal control pathways glycogen phosphorylase activity is modulated by epinephrine and glucagon which promote its conversion to the active form. Through these pathways phosphorylases exhibit dynamic responses to varying energy needs.

Glycogen phosphorylase is linked to McArdle's disease a glycogen storage disorder resulting from muscle phosphorylase deficiency. This condition causes muscle pain and weakness upon exertion. Another related disorder is Hers disease caused by liver glycogen phosphorylase deficiency resulting in hypoglycemia and hepatomegaly. Both conditions highlight the importance of the glycogen phosphorylase function for normal muscle and liver physiology.
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Product protocols

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Target data

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:113535 PubMed38060450

2023

Epinephrine inhibits PI3Kα via the Hippo kinases.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Yu Lin,Shakti Ramsamooj,Tiffany Perrier,Katarina Liberatore,Louise Lantier,Neil Vasan,Kannan Karukurichi,Seo-Kyoung Hwang,Edward A Kesicki,Edward R Kastenhuber,Thorsten Wiederhold,Tomer M Yaron,Emily M Huntsman,Mengmeng Zhu,Yilun Ma,Marcia N Paddock,Guoan Zhang,Benjamin D Hopkins,Owen McGuinness,Robert E Schwartz,Baran A Ersoy,Lewis C Cantley,Jared L Johnson,Marcus D Goncalves

Biochemistry 62:1360-1368 PubMed36989206

2023

Allosteric Regulation of Glycogen Phosphorylase by Order/Disorder Transition of the 250' and 280s Loops.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Kish,Sivaraman Subramanian,Victoria Smith,Natasha Lethbridge,Lindsay Cole,Frank Vollmer,Nicholas J Bond,Jonathan J Phillips

International journal of molecular sciences 23: PubMed36077341

2022

A Mouse Model of Glycogen Storage Disease Type IX-Beta: A Role for in Glycogenolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Charles J Arends,Lane H Wilson,Ana Estrella,Oh Sung Kwon,David A Weinstein,Young Mok Lee

Cells 11: PubMed35563842

2022

Glycogen Storage Disease Phenotypes Accompanying the Perturbation of the Methionine Cycle in NDRG3-Deficient Mouse Livers.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Ahm Sohn,Dong Chul Lee,Anna Park,Minho Kang,Byoung-Ha Yoon,Chul-Ho Lee,Yong-Hoon Kim,Kyoung-Jin Oh,Cha Yeon Kim,Seong-Hwan Park,Han Koo,Hyoung-Chin Kim,Won Kee Yoon,Dae-Sik Lim,Daesoo Kim,Kyung Chan Park,Young Il Yeom
View all publications
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