Glycolysis Stress Test Companion Assay (ab222945) allows glycolytic characterization at user-defined glucose concentrations in live cells when used in combination with our Glycolysis Assay [Extracellular Acidification] (ab197244).
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Key facts
- Detection method
- Fluorescent
- Sample types
- Adherent cells
- Assay type
- Cell-based (quantitative)
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Glycolysis Stress Test Companion Assay (ab222945) allows glycolytic characterization at user-defined glucose concentrations in live cells when used in combination with our Glycolysis Assay [Extracellular Acidification] (ab197244).
Key facts
- Detection method
- Fluorescent
- Sample types
- Adherent cells
- Assay type
- Cell-based (quantitative)
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Glycolysis Stress Test Companion Assay (ab222945) allows glycolytic characterization at user-defined glucose concentrations in live cells when used in combination with our Glycolysis Assay [Extracellular Acidification] (Glycolysis Assay [Extracellular acidification] ab197244).
The assay uses 2-Deoxyglucose (2-DG) and oligomycin to facilitate an in-depth analysis of cellular glycolytic flux: 2-DG blocks glycolysis through competitive hexokinase inhibition allowing quantification of non-glycolytic extracellular acidification (ECA); oligomycin inhibits ATP synthase, therefore preventing aerobic ATP generation and forcing cells to increase glycolytic flux to meet ATP demand. The levels of such compensatory glycolysis achievable under test conditions can reveal underlying glycolytic perturbation not evident under basal conditions and facilitates assessment of ATP-demand limited glycolysis capability. Glucose oxidase is included in the kit to provide a convenient cell-free ECA assay signal control.
Learn more about the full range of in live cells. Or review the full for other assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress.
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Functional Studies - Glycolysis Stress Test Companion Assay (ab222945)
Functional Studies - Glycolysis Stress Test Companion Assay (ab222945)
Functional Studies - Glycolysis Stress Test Companion Assay (ab222945)
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