Glycolysis Stress Test Complete Assay Kit (ab222946) offers a complete solution to measure glycolytic characterization at user-defined glucose concentrations in live cells using conventional fluorescence plate readers.
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- Nature communications 13:45272022Minimally invasive detection of cancer using metabolic changes in tumor-associated natural killer cells with Oncoimmune probes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeeptha Ishwar,Rupa Haldavnekar,Krishnan Venkatakrishnan,Bo TanPubMed 35927264
- Acta pharmacologica Sinica 43:157-1662021DUBR suppresses migration and invasion of human lung adenocarcinoma cells via ZBTB11-mediated inhibition of oxidative phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Nie,Min-Juan Hu,Qin Zhang,Jun Lu,Fang-Fei Qian,Le-le Zhang,Fang Hu,Chang-Hui Li,Shu-Hui Cao,Jing-Wen Li,Yue Wang,Xue-Yan Zhang,Mi-Die Xu,Bao-Hui HanPubMed 33758355
Key facts
- Detection method
- Fluorescent
- Sample types
- Adherent cells
- Assay type
- Cell-based (quantitative)
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Glycolysis Stress Test Complete Assay Kit (ab222946) offers a complete solution to measure glycolytic characterization at user-defined glucose concentrations in live cells using conventional fluorescence plate readers.
Key facts
- Detection method
- Fluorescent
- Sample types
- Adherent cells
- Assay type
- Cell-based (quantitative)
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Glycolysis Stress Test Complete Assay Kit (ab222946) offers a complete solution to measure glycolytic characterization at user-defined glucose concentrations in live cells using conventional fluorescence plate readers. This product combines Glycolysis Assay [Extracellular acidification] (Glycolysis Assay [Extracellular acidification] ab197244) and Glycolysis Stress Test Companion Assay (Glycolysis Stress Test Companion Assay ab222945) in one practical kit for single purchase.
The assay uses 2-Deoxyglucose (2-DG) and oligomycin to facilitate an in-depth analysis of cellular glycolytic flux: 2-DG blocks glycolysis through competitive hexokinase inhibition allowing quantification of non-glycolytic extracellular acidification (ECA); oligomycin inhibits ATP synthase, therefore preventing aerobic ATP generation and forcing cells to increase glycolytic flux to meet ATP demand. The levels of such compensatory glycolysis achievable under test conditions can reveal underlying glycolytic perturbation not evident under basal conditions and facilitates assessment of ATP-demand limited glycolysis capability. Glucose oxidase is included in the kit to provide a convenient cell-free ECA assay signal control.
Learn more about the full range of in live cells. Or review the full for other assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress.
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Functional Studies - Glycolysis Stress Test Complete Assay Kit (ab222946)
Functional Studies - Glycolysis Stress Test Complete Assay Kit (ab222946)
Functional Studies - Glycolysis Stress Test Complete Assay Kit (ab222946)
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