Golgi/ER Staining Kit | Cytopainter (ab139485) provides a rapid and convenient method for visualizing the Golgi and ER within living cells, without requiring lengthy transfection procedures.
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- International journal of oncology 62:2022Overcharged lipid metabolism in mechanisms of antitumor by ‑derived polysaccharide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiumin Li,Qiaoling Su,Yutian PanPubMed 36416344
- Scientific reports 6:291692016Nanoscale imaging of untreated mammalian cells in a medium with low radiation damage using scanning electron-assisted dielectric microscopy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomoko Okada et. al.PubMed 27375121
Key facts
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based
- Reactive species
- Mammals
What's included?
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Golgi/ER Staining Kit | Cytopainter (ab139485) provides a rapid and convenient method for visualizing the Golgi and ER within living cells, without requiring lengthy transfection procedures.
Key facts
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based
- Reactive species
- Mammals
- Assay Platform
- Fluorescence microscope
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- -80°C
Notes
Golgi/ER Staining Kit | Cytopainter (ab139485) provides a rapid and convenient method for visualizing the Golgi and ER within living cells, without requiring lengthy transfection procedures. ab139485 is validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line Jurkat, canine kidney cell line MDCK, and human bone osteosarcoma epithelial cell line U2OS. The kit should also be suitable for identifying Golgi body and endoplasmic reticulum perturbing agents and thus can be a useful tool for examining the transport and recycling of molecules from the GA to ER in cellular secretory pathways. Organelle Reagent III contains a mixture of GA-selective, ER-selective and nucleus-selective dyes suitable for live cell staining. Compared with other commercially available dyes for labeling Golgi bodies, the green dye component of the Reagent III is more faithfully localized to the Golgi apparatus, with minimal staining of the endoplasmic reticulum. The red dye component of the Reagent III stains the endoplasmic reticulum with high fidelity and is specifically designed for use with green fluorescing probes. Review other dyes and kits for , or the
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5 product images
This image is courtesy of a customer review submitted by Edwin HernandezFluorescence Microscopy - Golgi/ER Staining Kit - Cytopainter (ab139485)
CytoPainter Golgi/ER Staining Kit (ab139485) staining live rat astrocyte cells. Cells were seeded on a Ibidi u-slide 18 well flat poly-L-Lysine coated. Cells were washed with 1XAssay Solution, incubated for 30 min at 37° with the Organelle Reagent III, washed 3 times with ice cold medium (DMEM F-12) and incubated again in medium for 30 minutes at 37°.
ER (red), Golgi (green), Nuclear (blue).
Fluorescence Microscopy - Golgi/ER Staining Kit - Cytopainter (ab139485)
Absorbance and fluorescent emission - blue dye. Absorbance and fluorescent emission spectra for the blue dye
Fluorescence Microscopy - Golgi/ER Staining Kit - Cytopainter (ab139485)
Fluorescence image - red dye. Fluorescence excitation and emission spectra for the red dye
Fluorescence Microscopy - Golgi/ER Staining Kit - Cytopainter (ab139485)
Fluorescence image - green dye. Fluorescence excitation and emission spectra for the green dye
Fluorescence Microscopy - Golgi/ER Staining Kit - Cytopainter (ab139485)
Fluoroescent staining using Organelle Reagent III. ab139485 kit staining of MDCK epithelial cells. The ER (red), Golgi (green) and nuclear (blue) dyes highlight their respective subcellular targets with high dependability.
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