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AB139485

Golgi/ER Staining Kit - Cytopainter

5

(3 Reviews)

|

(2 Publications)

Golgi/ER Staining Kit | Cytopainter (ab139485) provides a rapid and convenient method for visualizing the Golgi and ER within living cells, without requiring lengthy transfection procedures.
5 Images
Fluorescence Microscopy - Golgi/ER Staining Kit - Cytopainter (AB139485)
  • Fluorescence Microscopy

AbReview39138****

Fluorescence Microscopy - Golgi/ER Staining Kit - Cytopainter (AB139485)

Fluoroescent staining using CytoPainter Golgi/ER Staining Kit (ab139485).

CytoPainter Golgi/ER Staining Kit (ab139485) staining live rat astrocyte cells. Cells were seeded on a Ibidi u-slide 18 well flat poly-L-Lysine coated. Cells were washed with 1XAssay Solution, incubated for 30 min at 37° with the Organelle Reagent III, washed 3 times with ice cold medium (DMEM F-12) and incubated again in medium for 30 minutes at 37°.

ER (red), Golgi (green), Nuclear (blue).

This image is courtesy of an Abreview submitted by Edwin Hernandez

Fluorescence Microscopy - Golgi/ER Staining Kit - Cytopainter (AB139485)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Golgi/ER Staining Kit - Cytopainter (AB139485)

Absorbance and fluorescent emission - blue dye. Absorbance and fluorescent emission spectra for the blue dye

Fluorescence Microscopy - Golgi/ER Staining Kit - Cytopainter (AB139485)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Golgi/ER Staining Kit - Cytopainter (AB139485)

Fluorescence image - red dye. Fluorescence excitation and emission spectra for the red dye

Fluorescence Microscopy - Golgi/ER Staining Kit - Cytopainter (AB139485)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Golgi/ER Staining Kit - Cytopainter (AB139485)

Fluoroescent staining using Organelle Reagent III. ab139485 kit staining of MDCK epithelial cells. The ER (red), Golgi (green) and nuclear (blue) dyes highlight their respective subcellular targets with high dependability.

Fluorescence Microscopy - Golgi/ER Staining Kit - Cytopainter (AB139485)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Golgi/ER Staining Kit - Cytopainter (AB139485)

Fluorescence image - green dye. Fluorescence excitation and emission spectra for the green dye

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Assay Platform

Fluorescence microscope

Product details

Golgi/ER Staining Kit | Cytopainter (ab139485) provides a rapid and convenient method for visualizing the Golgi and ER within living cells, without requiring lengthy transfection procedures. ab139485 is validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line Jurkat, canine kidney cell line MDCK, and human bone osteosarcoma epithelial cell line U2OS. The kit should also be suitable for identifying Golgi body and endoplasmic reticulum perturbing agents and thus can be a useful tool for examining the transport and recycling of molecules from the GA to ER in cellular secretory pathways. Organelle Reagent III contains a mixture of GA-selective, ER-selective and nucleus-selective dyes suitable for live cell staining. Compared with other commercially available dyes for labeling Golgi bodies, the green dye component of the Reagent III is more faithfully localized to the Golgi apparatus, with minimal staining of the endoplasmic reticulum. The red dye component of the Reagent III stains the endoplasmic reticulum with high fidelity and is specifically designed for use with green fluorescing probes. Review other dyes and kits for , or the

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab139485-100Test", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"10X Assay Buffer 1", "number":"AB139485-CMP02", "productcode":"" }, { "size":"1 x 1.2 mL", "name":"50X Assay Buffer 2", "number":"AB139485-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Organelle Reagent III (lyophilized)", "number":"AB139485-CMP01", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
-80°C

Product protocols

Target data

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 62: PubMed36416344

2022

Overcharged lipid metabolism in mechanisms of antitumor by ‑derived polysaccharide.

Applications

Unspecified application

Species

Unspecified reactive species

Xiumin Li,Qiaoling Su,Yutian Pan

Scientific reports 6:29169 PubMed27375121

2016

Nanoscale imaging of untreated mammalian cells in a medium with low radiation damage using scanning electron-assisted dielectric microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoko Okada,Toshihiko Ogura
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