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AB234044

Griess Assay Kit / Griess Reagent Kit

5

(1 Review)

|

(5 Publications)

Griess Assay Kit / Griess Reagent Kit ab234044 is a nitrite assay kit based on the Griess reaction. Includes nitrite standard for quantitation. Readout on any colorimetric (540 nm) plate reader.
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Functional Studies - Griess Assay Kit / Griess Reagent Kit (AB234044)
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Supplier Data

Functional Studies - Griess Assay Kit / Griess Reagent Kit (AB234044)

Nitrite concentration in pooled human serum and HeLa nuclear extracts.

Human serum was deproteinized using 10 kDa columns. Filtrate (50 μl, undiluted) was collected and assayed according to kit protocols. HeLa nuclear cells were homogenized using Nitrite Assay Buffer (100 μg protein).

Functional Studies - Griess Assay Kit / Griess Reagent Kit (AB234044)
  • FuncS

Supplier Data

Functional Studies - Griess Assay Kit / Griess Reagent Kit (AB234044)

Nitrite standard curve

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Urine, Plasma, Serum, Cell Lysate

Results type

Quantitative

Sensitivity

= 1 nmol/well

Assay time

1h 30m

Assay Platform

Microplate reader

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Product details

Griess Assay Kit / Griess Reagent Kit ab234044 uses a classic protocol for the estimation of nitrite in biological samples.

Griess assay principle
In the Griess reaction, Nitrite is reduced to nitrogen oxide using Griess Reagent I. Nitrogen oxide then reacts with Griess Reagent II forming a stable product that can be detected by its absorbance at 540 nm.
Griess Reagent I is 1% Sulfanilamide in 5% Phosphoric Acid.
Griess Reagent II is 0.1% NED (N-(1-Naphthyl)ethylenediamine dihydrochloride) in water.

The two-step assay is simple, fast and can detect nitrite levels as low as 1 nmol/well.

Griess assay protocol summary
- add samples and standards to wells
- sequentially add Griess Reagent I, Griess Reagent II, assay buffer to wells
- incubate for 10-min at room temperature
- analyse on a plate reader

Related Nitrite / Nitrate assays
- To measure total nitrate/nitrite levels, use Nitric Oxide Assay Kit ab65328.

Other Notes
This product was previously called K544 Biovision Nitrite Assay Kit (Griess Reagent). Abcam acquired Biovision in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab234044-200Test", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Buffer III", "number":"AB234044-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Nitrite Standard", "number":"AB234044-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"Griess Reagent II", "number":"AB234044-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"Griess Reagent I", "number":"AB234044-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Please refer to protocols
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Product protocols

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Target data

See full target information Nitrite
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of medicinal food 24:852-859 PubMed34382871

2021

The Decursin Analog, CYJ-27, Suppresses Inflammation Via the Downregulation of NF-B and STAT-1.

Applications

Unspecified application

Species

Unspecified reactive species

Wonhwa Lee,Hyunchae Sim,Yoon-Jung Choi,Ju Young Seo,Mi-Young Yun,Gyu Yong Song,Jong-Sup Bae

American journal of physiology. Lung cellular and molecular physiology 320:L508-L521 PubMed33502933

2021

Glucose-6-phosphate dehydrogenase deficiency contributes to metabolic abnormality and pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Mathews Valuparampil Varghese,Joel James,Olga Rafikova,Ruslan Rafikov

Molecular metabolism 43:101123 PubMed33227495

2020

Inhibition of mitochondrial fission and iNOS in the dorsal vagal complex protects from overeating and weight gain.

Applications

Unspecified application

Species

Unspecified reactive species

Bianca Patel,Lauryn E New,Joanne C Griffiths,Jim Deuchars,Beatrice M Filippi

Micromachines 11: PubMed33143339

2020

A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture.

Applications

Unspecified application

Species

Unspecified reactive species

Samineh Barmaki,Daniela Obermaier,Esko Kankuri,Jyrki Vuola,Sami Franssila,Ville Jokinen

The Journal of clinical investigation 129:4261-4275 PubMed31483289

2019

Lactoferrin-induced myeloid-derived suppressor cell therapy attenuates pathologic inflammatory conditions in newborn mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yufeng Liu,Michela Perego,Qiang Xiao,Yumei He,Shuyu Fu,Juan He,Wangkai Liu,Xing Li,Yanlai Tang,Xiaoyu Li,Weiming Yuan,Wei Zhou,Fan Wu,Chunhong Jia,Qiliang Cui,George S Worthen,Erik A Jensen,Dmitry I Gabrilovich,Jie Zhou
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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