Griess Reagent Kit ab234044 uses a classic protocol for the estimation of nitrite in biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Journal of medicinal food 24:852-8592021The Decursin Analog, CYJ-27, Suppresses Inflammation Via the Downregulation of NF-B and STAT-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWonhwa Lee,Hyunchae Sim,Yoon-Jung Choi,Ju Young Seo,Mi-Young Yun,Gyu Yong Song,Jong-Sup BaePubMed 34382871
- American journal of physiology. Lung cellular and molecular physiology 320:L508-L5212021Glucose-6-phosphate dehydrogenase deficiency contributes to metabolic abnormality and pulmonary hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMathews Valuparampil Varghese,Joel James,Olga Rafikova,Ruslan RafikovPubMed 33502933
- Molecular metabolism 43:1011232020Inhibition of mitochondrial fission and iNOS in the dorsal vagal complex protects from overeating and weight gain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBianca Patel,Lauryn E New,Joanne C Griffiths,Jim Deuchars,Beatrice M FilippiPubMed 33227495
- Micromachines 11:2020A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamineh Barmaki et. al.PubMed 33143339
- The Journal of clinical investigation 129:4261-42752019Lactoferrin-induced myeloid-derived suppressor cell therapy attenuates pathologic inflammatory conditions in newborn mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYufeng Liu,Michela Perego,Qiang Xiao,Yumei He,Shuyu Fu,Juan He,Wangkai Liu,Xing Li,Yanlai Tang,Xiaoyu Li,Weiming Yuan,Wei Zhou,Fan Wu,Chunhong Jia,Qiliang Cui,George S Worthen,Erik A Jensen,Dmitry I Gabrilovich,Jie ZhouPubMed 31483289
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Urine, Plasma, Serum, Cell Lysate
- Assay type
Quantitative
- Assay time
1h 30m
- Sensitivity
= 1 nmol/well
Target data
What's included?
Recommended products
Griess Reagent Kit ab234044 uses a classic protocol for the estimation of nitrite in biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Urine, Plasma, Serum, Cell Lysate
- Assay type
Quantitative
- Assay time
1h 30m
- Assay Platform
Microplate reader
- Sensitivity
= 1 nmol/well
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Please refer to protocols
Notes
Griess Reagent Kit ab234044 uses a classic protocol for the estimation of nitrite in biological samples.
Nitrite is reduced to nitrogen oxide using Griess Reagent I. Nitrogen oxide then reacts with Griess Reagent II forming a stable product that can be detected by its absorbance at 540 nm.
The two-step assay is simple, fast and can detect nitrite levels as low as 1 nmol/well.
This product is manufactured by BioVision, an Abcam company and was previously called K544 Nitrite Assay Kit (Griess Reagent). K544-200 is the same size as the 200 test size of ab234044.
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2 product images
Functional Studies - Griess reagent (ab234044)
Functional Studies - Griess reagent (ab234044)
Nitrite concentration in pooled human serum and HeLa nuclear extracts.
Human serum was deproteinized using 10 kDa columns. Filtrate (50 μl, undiluted) was collected and assayed according to kit protocols. HeLa nuclear cells were homogenized using Nitrite Assay Buffer (100 μg protein).
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Product protocols
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