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Griess Reagent Kit ab234044 uses a classic protocol for the estimation of nitrite in biological samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.


Images

Functional Studies - Griess reagent (AB234044), expandable thumbnail
  • Functional Studies - Griess reagent (AB234044), expandable thumbnail

Publications

Key facts

Detection method
Colorimetric
Sample types
Tissue Lysate, Urine, Plasma, Serum, Cell Lysate
Assay type
Quantitative
Assay time
1h 30m
Sensitivity
= 1 nmol/well

What's included?

200 Test
Components
Buffer III
1 x 30 mL
Griess Reagent I
1 x 10 mL
Griess Reagent II
1 x 10 mL
Nitrite Standard
1 x 1 Vial

Recommended products

Griess Reagent Kit ab234044 uses a classic protocol for the estimation of nitrite in biological samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Detection method
Colorimetric
Sample types
Tissue Lysate, Urine, Plasma, Serum, Cell Lysate
Assay type
Quantitative
Assay time
1h 30m
Assay Platform
Microplate reader
Sensitivity
= 1 nmol/well

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Please refer to protocols

Notes

Griess Reagent Kit ab234044 uses a classic protocol for the estimation of nitrite in biological samples.

Nitrite is reduced to nitrogen oxide using Griess Reagent I. Nitrogen oxide then reacts with Griess Reagent II forming a stable product that can be detected by its absorbance at 540 nm.

The two-step assay is simple, fast and can detect nitrite levels as low as 1 nmol/well.

This product is manufactured by BioVision, an Abcam company and was previously called K544 Nitrite Assay Kit (Griess Reagent). K544-200 is the same size as the 200 test size of ab234044.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - Griess reagent (ab234044), expandable thumbnail

    Functional Studies - Griess reagent (ab234044)

  • Functional Studies - Griess reagent (ab234044), expandable thumbnail

    Functional Studies - Griess reagent (ab234044)

    Nitrite concentration in pooled human serum and HeLa nuclear extracts.

    Human serum was deproteinized using 10 kDa columns. Filtrate (50 μl, undiluted) was collected and assayed according to kit protocols. HeLa nuclear cells were homogenized using Nitrite Assay Buffer (100 μg protein).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com