GSH/GSSG Ratio Detection Assay Kit (Fluorometric - Green)

• FuncS

Lab

Functional Studies - GSH/GSSG Ratio Detection Assay Kit (Fluorometric - Green) (AB138881)

Total GSH measured in cell lysates showing quantity (umol) per 1 mln cells.

Samples with the concentration of 1e7-1e8 cells/mL were used. Samples were diluted 10-1000 fold.

• FuncS

Lab

Functional Studies - GSH/GSSG Ratio Detection Assay Kit (Fluorometric - Green) (AB138881)

GSH in reduced state measured in cell lysates showing quantity (umol) per 1 mln cells.

Samples with the concentration of 1e7-1e8 cells/mL were used. Samples were diluted 10-1000 fold.

• FuncS

Lab

Functional Studies - GSH/GSSG Ratio Detection Assay Kit (Fluorometric - Green) (AB138881)

GSH in reduced state measured in tissue lysates showing quantity (umol) per miligram of extracted protein of tested sample.

Protein concentration for samples varied from 6 mg/mL to 16 mg/mL. Samples were diluted 10-100 fold.

• FuncS

Lab

Functional Studies - GSH/GSSG Ratio Detection Assay Kit (Fluorometric - Green) (AB138881)

Total GSH measured in tissue lysates showing quantity (umol) per miligram of extracted protein of tested sample.

Protein concentration for samples varied from 6 mg/mL to 16 mg/mL. Samples were diluted 10-100 fold.

• FuncS

Lab

Functional Studies - GSH/GSSG Ratio Detection Assay Kit (Fluorometric - Green) (AB138881)

GSH in reduced state measured in biological fluids showing concentration (uM) in tested samples. Human samples were diluted 10 fold. Rat sample was diluted 10-1000 fold.

• FuncS

Lab

Functional Studies - GSH/GSSG Ratio Detection Assay Kit (Fluorometric - Green) (AB138881)

Total GSH measured in biological fluids showing concentration (uM) in tested samples. Samples were diluted 10-100 fold.

• FuncS

Unknown

Functional Studies - GSH/GSSG Ratio Detection Assay Kit (Fluorometric - Green) (AB138881)

Measurement of reduced (GSH) and oxidized (GSSG) forms of glutathione in myotubes treated with 150 μM TBHP for 1 h (n = 6).

Biesemann, Nadine et al., Scientific reports: vol. 8,1 9408., Fig 4, doi:10.1038/s41598-018-27614-8

• Biochemical assay

PubMed

Biochemical assay - GSH/GSSG Ratio Detection Assay Kit (Fluorometric - Green) (AB138881)

Niu et al used Lipid Peroxidation (MDA) Assay Kit ab118970, GSH/ GSSG Assay Kit ab138881, and Glutathione Peroxidase Assay Kit ab125066 to investigate the use of porous Se@SiO2 nanospheres to achieve controlled release of selenium, a scavenger of intracellular free radicals, in mouse eyes.

Male diabetic db/db and control db/m mice were injected in the eyes with porous Se@SiO2 nanospheres, and porous SiO2 nanospheres (NPs) without Se.

Porous Se@SiO2 nanospheres inhibit diabetes-induced retinal lipid peroxidation and inflammation. Levels of MDA in retinal homogenates (n = 6). Expression levels of GPX4 in retinas were measured using western blotting; β-actin was used as a loading control (left panel). Band densities were assessed using the ImageJ software, and GPX4 expression levels are represented as their ratios to β-actin (right panel) (n = 3). Levels of GSH, GSSG, and the ratio of GSH to GSSG in retinal homogenates (n = 6). Data are represented as the mean ± SD. **p < 0.01, ***p < 0.001; ns, nonsignificant.

Retinal samples were harvested, washed, and lysed according to the manufacturer's instructions. Protein concentrations were determined using a bicinchoninic acid kit. MDA and glutathione concentrations were determined using a lipid peroxidation MDA assay kit, reduced glutathione (GSH) / oxidized glutathione (GSSG) Ratio Detection Assay kit (ab118970, ab138881; Abcam, MA, USA), and a microplate reader. The relative concentrations of MDA and glutathione were calculated by normalizing the measured concentrations to that of the total protein.

• FuncS

Supplier Data

Functional Studies - GSH/GSSG Ratio Detection Assay Kit (Fluorometric - Green) (AB138881)

GSH and Total (GSH+GSSG) dose responses were measured with ab138881. Blue line : in the presence of GSH only; Red line : in the presence of 1 : 1 GSH/GSSG.