GSH/GSSG Ratio Detection Assay Kit II ab205811 is used to quantify total glutathione, GSH and GSSG. Simply add probe(s) and incubate at room temperature for 10-60 mins. Readout on any fluorometric (Ex/Em = 490/520 nm) plate reader.
- Cited in >60 publications
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- Chemical biology & drug design 103:e144892024Cinnamaldehyde protects cardiomyocytes from oxygen-glucose deprivation/reoxygenation-induced lipid peroxidation and DNA damage via activating the Nrf2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan-Guang Li,Jiang-Hong Li,Hai-Qin Wang,Junhua Liao,Xiao-Ya DuPubMed 38404216
- World journal of stem cells 15:1017-10342023Dissecting molecular mechanisms underlying ferroptosis in human umbilical cord mesenchymal stem cells: Role of cystathionine γ-lyase/hydrogen sulfide pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Hu,Xiang-Xi Zhang,Tao Zhang,Wan-Cheng YuPubMed 38058959
- Molecular medicine (Cambridge, Mass.) 29:1402023Targeted inhibition of CX3CL1 limits podocytes ferroptosis to ameliorate cisplatin-induced acute kidney injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiming Gong,Tengfang Lai,Liudan Liang,Yan Jiang,Fahui LiuPubMed 37875838
- Frontiers in pharmacology 14:12331842023Oxidative stress in rat brain during experimental status epilepticus: effect of antioxidants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarius Fuchs,Christian Viel,Alina Lehto,Helene Lau,Jochen KleinPubMed 37767398
- Chinese medicine 18:1102023Gegen Qinlian decoction (GQD) inhibits ulcerative colitis by modulating ferroptosis-dependent pathway in mice and organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue Wang,Jianye Quan,Chengkui Xiu,Jiali Wang,Jiaqi ZhangPubMed 37649073
- Journal of experimental & clinical cancer research : CR 42:1902023TNFAIP2 confers cisplatin resistance in head and neck squamous cell carcinoma via KEAP1/NRF2 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTeng Xu,Yuemei Yang,Zhihong Chen,Jinsong Wang,Xiaolei Wang,Yang Zheng,Chao Wang,Yachen Wang,Zaiou Zhu,Xu Ding,Junbo Zhou,Gang Li,Hongchuang Zhang,Wei Zhang,Yunong Wu,Xiaomeng SongPubMed 37525222
- Nature communications 14:38232023Increased glucose availability sensitizes pancreatic cancer to chemotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAli Vaziri-Gohar,Jonathan J Hue,Ata Abbas,Hallie J Graor,Omid Hajihassani,Mehrdad Zarei,George Titomihelakis,John Feczko,Moeez Rathore,Sylwia Chelstowska,Alexander W Loftus,Rui Wang,Mahsa Zarei,Maryam Goudarzi,Renliang Zhang,Belinda Willard,Li Zhang,Adam Kresak,Joseph E Willis,Gi-Ming Wang,Curtis Tatsuoka,Joseph M Salvino,Ilya Bederman,Henri Brunengraber,Costas A Lyssiotis,Jonathan R Brody,Jordan M WinterPubMed 37380658
- Antioxidants (Basel, Switzerland) 12:2023Defects in Glutathione System in an Animal Model of Amyotrophic Lateral Sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFranziska T Wunsch,Nils Metzler-Nolte,Carsten Theiss,Veronika MatschkePubMed 37237880
- Cell death & disease 14:7820233-Carboxy-4-methyl-5-propyl-2-furanpropanoic acid (CMPF) induces cell death through ferroptosis and acts as a trigger of apoptosis in kidney cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJung Sun Park,Dong-Hyun Kim,Hoon-In Choi,Chang Seong Kim,Eun Hui Bae,Seong Kwon Ma,Soo Wan KimPubMed 36732325
- Cancer cell 41:356-372.e102023Neutrophil-activating therapy for the treatment of cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIan L Linde,Tyler R Prestwood,Jingtao Qiu,Genay Pilarowski,Miles H Linde,Xiangyue Zhang,Lei Shen,Nathan E Reticker-Flynn,David Kung-Chun Chiu,Lauren Y Sheu,Simon Van Deursen,Lorna L Tolentino,Wen-Chao Song,Edgar G EnglemanPubMed 36706760
Key facts
- Detection method
- Fluorescent
- Sample types
- Urine, Plasma, Tissue Extracts, Cell Lysate
- Assay type
- Quantitative
- Assay time
- 30m
- Sensitivity
- = 10 nM
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GSH/GSSG Ratio Detection Assay Kit II ab205811 is used to quantify total glutathione, GSH and GSSG. Simply add probe(s) and incubate at room temperature for 10-60 mins. Readout on any fluorometric (Ex/Em = 490/520 nm) plate reader.
- Cited in >60 publications
Key facts
- Detection method
- Fluorescent
- Sample types
- Urine, Plasma, Tissue Extracts, Cell Lysate
- Assay type
- Quantitative
- Assay time
- 30m
- Assay Platform
- Microplate reader
- Sensitivity
- = 10 nM
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
How the assay works
The GSH/GSSG assay protocol uses a proprietary non-fluorescent water-soluble dye that becomes strongly fluorescent upon reacting with GSH. With a one-step fluorimetric method, the kit can detect as little as 1 picomole of GSH or GSSG in a 100 µL assay volume.
The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and readily adapted to automation without a separation step. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/520 nm.
GSH/GSSG assay protocol summary:
- add samples (deproteinized) and standards to wells
- for GSH assay add Thiol Green in assay buffer, or for total glutathione (GSH + GSSG) assay also add GSSG probe
- incubate for 10 - 60 min at room temp
- analyze with microplate reader
GSSG levels can be calculated by subtracting GSH from total glutathione levels.
NOTE: For measuring GSH Standard only, there is enough reagent provided to perform 200 tests.
How other researchers are using
GSH/GSSG Ratio Detection Assay Kit II (Fluorometric - Green) has been used in a variety of sample type including:
- Human umbilical cord mesenchymal stem cells (hUCMSCs) 1
- Mouse kidney tissues 2
- Rat Brain homogenates 3
References:
1 - Hu B et al. 2023; 2 - Gong Q et al. 2023; 3 - Fuchs M et al. 2023.
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3 product images
Functional Studies - GSH/GSSG Ratio Detection Assay Kit II (Fluorometric - Green) (ab205811)
GSH/GSSG Ratio Detection Assay Kit.
Reduced GSH and total GSH levels in cell lysates. Cells lysed to the concentration of 1e7 cells per mL and tested diluted 6-54 fold.
* Cells serum starved for 24 hours.
Functional Studies - GSH/GSSG Ratio Detection Assay Kit II (Fluorometric - Green) (ab205811)
Sample Calibration Curve - Reduced GSH.
Reduced GSH dose responses were measured in a black 96-well plate with ab205811 using a fluorescence microplate reader. 50 μL of GSH standards (0.01 to 5 μM) or blank control was added into each well, and then 50 μL of GSH Assay Mixture was added. The fluorescence intensity was measured at Ex/Em = 490/520 nm after 30 minutes incubation.
Functional Studies - GSH/GSSG Ratio Detection Assay Kit II (Fluorometric - Green) (ab205811)
Sample Calibration Curve - Total GSH.
Total GSH dose responses were measured with ab205811 in a black 96-well plate using a fluorescence microplate reader. 50 μL of GSSG standards (0.01 to 5 μM), GSH-containing samples or blank control were added into each well, and then 50 μL of Total GSH Reaction Mixture was added. Fluorescence intensity was measured at Ex/Em = 490/520 nm after 30 minutes incubation.
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