GSH/GSSG Ratio Detection Assay Kit II (Fluorometric - Green)

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Functional Studies - GSH/GSSG Ratio Detection Assay Kit II (Fluorometric - Green) (AB205811)

GSH/GSSG Ratio Detection Assay Kit.

Reduced GSH and total GSH levels in cell lysates. Cells lysed to the concentration of 1e7 cells per mL and tested diluted 6-54 fold.

* Cells serum starved for 24 hours.

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Functional Studies - GSH/GSSG Ratio Detection Assay Kit II (Fluorometric - Green) (AB205811)

Sample Calibration Curve - Reduced GSH.

Reduced GSH dose responses were measured in a black 96-well plate with ab205811 using a fluorescence microplate reader. 50 μL of GSH standards (0.01 to 5 μM) or blank control was added into each well, and then 50 μL of GSH Assay Mixture was added. The fluorescence intensity was measured at Ex/Em = 490/520 nm after 30 minutes incubation.

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Supplier Data

Functional Studies - GSH/GSSG Ratio Detection Assay Kit II (Fluorometric - Green) (AB205811)

Sample Calibration Curve - Total GSH.

Total GSH dose responses were measured with ab205811 in a black 96-well plate using a fluorescence microplate reader. 50 μL of GSSG standards (0.01 to 5 μM), GSH-containing samples or blank control were added into each well, and then 50 μL of Total GSH Reaction Mixture was added. Fluorescence intensity was measured at Ex/Em = 490/520 nm after 30 minutes incubation.