GSH+GSSG / GSH Assay Kit (Colorimetric) ab239709 provides a convenient, colorimetric method for analyzing either total glutathione or the reduced form glutathione alone using a microtiter plate reader.
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- Cell biology and toxicology 40:32024POU6F1 promotes ferroptosis by increasing lncRNA-CASC2 transcription to regulate SOCS2/SLC7A11 signaling in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingyun Wang,Qiaoyu Jia,Shuqin Jiang,Wenquan Lu,Hanbing NingPubMed 38267746
- iScience 26:1085662023Suppression of the - axis shifts arginine into the phosphocreatine energy system in pancreatic cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEros Di Giorgio,Himanshi Choudhary,Annalisa Ferino,Ylenia Cortolezzis,Emiliano Dalla,Francesca D'Este,Marina Comelli,Valentina Rapozzi,Luigi E XodoPubMed 38144458
- Molecular medicine (Cambridge, Mass.) 29:1372023Resveratrol reduces ROS-induced ferroptosis by activating SIRT3 and compensating the GSH/GPX4 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingjie Wang,Tianli Shen,Jie Lian,Kai Deng,Chao Qu,Enmeng Li,Gan Li,Yiwei Ren,Zijun Wang,Zhengdong Jiang,Xuejun Sun,Xuqi LiPubMed 37858064
- Environmental toxicology 38:2476-24862023Cisplatin triggers oxidative stress, apoptosis and pro-inflammatory responses by inhibiting the SIRT1-mediated Nrf2 pathway in chondrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPei-Ling Hsieh,Kun-Ling Tsai,Wan-Ching Chou,Chin-Hsien Wu,I-Ming Jou,Yuan-Kun Tu,Ching-Hou MaPubMed 37497868
- International journal of molecular sciences 24:2023Opioids Alleviate Oxidative Stress via the Nrf2/HO-1 Pathway in LPS-Stimulated Microglia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkash Shivling Mali,Ondrej Honc,Lucie Hejnova,Jiri NovotnyPubMed 37446267
- Cancer science 114:3301-33172023Growth differentiation factor 15 induces cisplatin resistance through upregulation of xCT expression and glutathione synthesis in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheng-Fan Wang,Yuh-Lih Chang,Wen-Liang Fang,Anna Fen-Yau Li,Chian-Feng Chen,Tien-Shun Yeh,Giun-Yi Hung,Kuo-Hung Huang,Hsin-Chen LeePubMed 37260027
- Molecules (Basel, Switzerland) 28:2023Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbdullah Alsrhani,Abozer Y Elderdery,Badr Alzahrani,Nasser A N Alzerwi,Maryam Musleh Althobiti,Musaed Rayzah,Bandar Idrees,Ahmed M E Elkhalifa,Suresh K Subbiah,Pooi Ling MokPubMed 37049991
- Antioxidants (Basel, Switzerland) 12:2023Rutin Protects Fibroblasts from UVA Radiation through Stimulation of Nrf2 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisabetta Tabolacci,Giuseppe Tringali,Veronica Nobile,Sara Duca,Michela Pizzoferrato,Patrizia Bottoni,Maria Elisabetta ClementiPubMed 37107196
- Pulmonary circulation 13:e122052023Transpulmonary amino acid metabolism in the sugen hypoxia model of pulmonary hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicolas Philip,Hongyang Pi,Mahin Gadkari,Xin Yun,John Huetsch,Cissy Zhang,Robert Harlan,Aurelie Roux,David Graham,Larissa Shimoda,Anne Le,Scott Visovatti,Peter J Leary,Sina A Gharib,Catherine Simpson,Lakshmi Santhanam,Jochen Steppan,Karthik SureshPubMed 36873460
- Journal of medical virology 95:e285762023Oxidative stress-A key determinant of complications and negative outcome in hepatitis E virus infected pregnancies: A comprehensive account involving cases from northeast India.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatasha Kashyap,Mafidul Islam,Harpreet Kaur,Diptika Tiwari,Anjuma Begum,Moumita Bose,Chandana Ray Das,Anjan Kumar Saikia,Pankaj Kalita,Purabi Deka Bose,Sujoy BosePubMed 36779361
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Serum, Cell culture extracts
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2 products for Alternative Product
Target data
Alternative names
GSH, Oxidised glutathione, Reduced glutathione
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GSH+GSSG / GSH Assay Kit (Colorimetric) ab239709 provides a convenient, colorimetric method for analyzing either total glutathione or the reduced form glutathione alone using a microtiter plate reader.
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Serum, Cell culture extracts
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
GSH+GSSG / GSH Assay Kit (Colorimetric) (ab239709) provides a convenient, colorimetric method for analyzing either total glutathione or the reduced form glutathione alone using a microtiter plate reader.
The assay is based on the glutathione recycling system by DTNB and glutathione reductase. DTNB and glutathione (GSH) react to generate 2-nitro-5-thiobenzoic acid which has a yellow color. Therefore, GSH concentration can be determined by measuring absorbance at 412 nm.
The generated GSSG can be reduced back to GSH by glutathione reductase, and GSH reacts with DTNB again to produce more 2-nitro-5-thiobenzoic acid. Therefore, the recycling system dramatically improves the sensitivity of total glutathione detection.
The kit includes the 5-Sulfosalicylic acid (SSA) for the removal of proteins from samples and for the protection of GSH oxidation and g-glutamyl transpeptidase reaction.
The kit can quantify glutathione from 1-100 ng/well in a 200 µL reaction. For detecting lower glutathione concentrations, such as in blood samples, increasing reaction time will generate stronger signal.
The kit can also specifically detect the reduced form of glutathione (GSH) by omitting the glutathione reductase from the reaction mixture. The sensitivity for detecting the reduced form of glutathione (without recycling system) is 100 times lower than detecting the total glutathione.
This product is manufactured by BioVision, an Abcam company and was previously called K261 Glutathione Colorimetric Assay Kit. K261-100 is the same size as the 100 test size of ab239709.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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Functional Studies - GSH+GSSG / GSH Assay Kit (Colorimetric) (ab239709)
Glutathione Standard Curve.
Various amounts of standard glutathione was added to the glutathione reaction and incubated for 10 min according to the kit instructions. Absorbance was measured at O.D. 405 nm.
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