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AB239709

GSH+GSSG / GSH Assay Kit (Colorimetric)

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(44 Publications)

GSH+GSSG / GSH Assay Kit (Colorimetric) ab239709 provides a convenient method to quantify either total glutathione, or the reduced form glutathione alone, using any colorimetric (412 nm) plate reader.

- Cited in >40 publications
2 Images
Functional Studies - GSH+GSSG / GSH Assay Kit (Colorimetric) (AB239709)
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Functional Studies - GSH+GSSG / GSH Assay Kit (Colorimetric) (AB239709)

Glutathione Standard Curve.

Various amounts of standard glutathione was added to the glutathione reaction and incubated for 10 min according to the kit instructions. Absorbance was measured at O.D. 405 nm.

Other - GSH+GSSG / GSH Assay Kit (Colorimetric) (AB239709)
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Supplier Data

Other - GSH+GSSG / GSH Assay Kit (Colorimetric) (AB239709)

Representative image of GSH+GSSG / GSH Assay Kit (Colorimetric) ab239709

Components shown from left to right :

- DTNB (2 vials)

- NADPH Generating Mix (2 vials)

- GSH Standard (2 vials)

- Glutathione Reductase (2 vials)

- Sulfosalicylic Acid

- Glutathione Reaction Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Cell culture extracts

Assay Platform

Microplate reader

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Product details

GSH+GSSG / GSH Assay Kit (Colorimetric) (ab239709) provides a convenient, colorimetric method for analyzing either total glutathione or the reduced form glutathione alone using a microtiter plate reader.

The assay is based on the glutathione recycling system by DTNB and glutathione reductase. DTNB and glutathione (GSH) react to generate 2-nitro-5-thiobenzoic acid which has a yellow color. Therefore, GSH concentration can be determined by measuring absorbance at 412 nm.

The generated GSSG can be reduced back to GSH by glutathione reductase, and GSH reacts with DTNB again to produce more 2-nitro-5-thiobenzoic acid. Therefore, the recycling system dramatically improves the sensitivity of total glutathione detection.

The kit includes the 5-Sulfosalicylic acid (SSA) for the removal of proteins from samples and for the protection of GSH oxidation and g-glutamyl transpeptidase reaction.

The kit can quantify glutathione from 1-100 ng/well in a 200 µL reaction. For detecting lower glutathione concentrations, such as in blood samples, increasing reaction time will generate stronger signal.

The kit can also specifically detect the reduced form of glutathione (GSH) by omitting the glutathione reductase from the reaction mixture. The sensitivity for detecting the reduced form of glutathione (without recycling system) is 100 times lower than detecting the total glutathione.

This product is manufactured by BioVision, an Abcam company and was previously called K261 Glutathione Colorimetric Assay Kit. K261-100 is the same size as the 100 test size of ab239709.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

See full target information Glutathione
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Publications (44)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutics 17: PubMed40573993

2025

Curcumin-Induced Apoptotic Cell Death in Human Glioma Cells Is Enhanced by Clusterin Deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Pinky Sultana,Jiri Novotny

Research (Washington, D.C.) 8:0703 PubMed40416363

2025

Targeting the Exonic Circular RNA/O-GlcNAc Transferase/Forkhead Box C1 Axis Inhibits Asparagine- and Alanine-Mediated Ferroptosis Repression in Neuroblastoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Qilan Li,Yang Cheng,Chunhui Yang,Minxiu Tian,Xiaojing Wang,Dan Li,Xinyue Li,Jiaying Qu,Shunchen Zhou,Liduan Zheng,Qiangsong Tong

Journal of proteome research 24:2771-2782 PubMed40360154

2025

Upregulations of SNAT2 and GLS-1 Are Key Osmoregulatory Responses of Human Corneal Epithelial Cells to Hyperosmotic Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Kenrick Kai-Yuen Chan,Alan Chun-Kit Lee,Shing-Yan Roy Chung,Man-Sau Wong,Chi-Wai Do,Thomas Chuen Lam,Hang-Kin Kong

Antioxidants (Basel, Switzerland) 13: PubMed39594565

2024

Low-Dose Melittin Enhanced Pigment Production Through the Upregulation of Tyrosinase Activity and Dendricity in Melanocytes by Limiting Oxidative Stress: A Therapeutic Implication for Vitiligo.

Applications

Unspecified application

Species

Unspecified reactive species

Manoj Kumar Tembhre, Shipra

The British journal of nutrition :1-15 PubMed39468832

2024

Evaluation of a probiotic blend on psychosocial health and biomarkers of inflammatory, immune and stress response in adults with subthreshold depression: a double-blind, randomised, placebo-controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

George Moschonis,Katerina Sarapis,Stephanie Resciniti,Renate Hall,Kanny Yim,Matilda Tonkovic,Clare Fitzgerald,Fay Anixiadis,Antony Vinh,Quynh Nhu Dinh,Rachael A Cronin,Matthew W Hale,Bradley J Wright,Marco Pane,Caroline J Tuck,Jessica R Biesiekierski

Antioxidants (Basel, Switzerland) 13: PubMed39456422

2024

Oxidative Stress in Polycystic Ovary Syndrome: Impact of Combined Oral Contraceptives.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolás Santander,Esteban G Figueroa,Alejandro González-Candia,Manuel Maliqueo,Bárbara Echiburú,Nicolás Crisosto,Francisca Salas-Pérez

Biomedicines 12: PubMed39457585

2024

Deferasirox's Anti-Chemoresistance and Anti-Metastatic Effect on Non-Small Cell Lung Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yamixa Delgado,Anamaris Torres-Sanchez,Daraishka Perez,Grace Torres,Sthephanie Estrada,Natalia Ortiz Alvelo,Jaisy Vega,Laurie Santos,Aracelis Torres,Bismark A Madera,Yancy Ferrer-Acosta

BMC cardiovascular disorders 24:531 PubMed39354361

2024

The role and possible mechanism of the ferroptosis-related SLC7A11/GSH/GPX4 pathway in myocardial ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Bingxin Chen,Ping Fan,Xue Song,Mingjun Duan

Nature communications 15:8226 PubMed39300060

2024

MFSD7C protects hemolysis-induced lung impairments by inhibiting ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Huirui Wang,Xiaona You,Jingcheng Wang,Xinyi Chen,Yinghui Gao,Mengmeng Wang,Wenru Zhang,Jiaozhen Zhang,Yang Yu,Bo Han,Mei Qi,Xiaohui Liu,Hongxiang Lou,Ting Dong

Current research in pharmacology and drug discovery 6:100186 PubMed38846010

2024

The polyphenolic compound punicalagin protects skin fibroblasts from UVA radiation oxidative damage.

Applications

Unspecified application

Species

Unspecified reactive species

Giada Bianchetti,Patrizia Bottoni,Giuseppe Tringali,Giuseppe Maulucci,Elisabetta Tabolacci,Maria Elisabetta Clementi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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