GST Activity Assay Kit (Fluorometric) provides a simple, fluorescence-based in vitro assay for detecting GST activity using fluorescence plate reader.
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- Frontiers in physiology 13:10644762022Elevated antioxidant defence in the brain of deep-diving pinnipeds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGerrit A Martens,Lars P Folkow,Thorsten Burmester,Cornelia GeßnerPubMed 36589435
- Experimental & molecular medicine 49:e3182017Inhibition of glutathione metabolism attenuates esophageal cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Peng et. al.PubMed 28428633
- Human vaccines & immunotherapeutics 8:765-762012Advances in vaccines against neglected tropical diseases: enhancing physical stability of a recombinant hookworm vaccine through biophysical and formulation studies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJordan L Plieskatt,Wanderson C Rezende,Chris M Olsen,Jared M Trefethen,Sangeeta B Joshi,C Russell Middaugh,Peter J Hotez,Maria Elena BottazziPubMed 22495115
Key facts
- Detection method
- Fluorescent
- Sample types
- Purified protein, Tissue Lysate, Saliva, Urine, Plasma, Serum, Other biological fluids, Cell Lysate
- Assay type
- Enzyme activity
- Assay time
- 2h
- Sensitivity
- < 0.5 mU/well
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Target data
Function
Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis (PubMed:11152686). Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid (PubMed:16624487).
Alternative names
Glutathione S-transferase A1, 13-hydroperoxyoctadecadienoate peroxidase, GST HA subunit 1, GST class-alpha member 1, GST-epsilon, GSTA1-1, GTH1, GSTA1
What's included?
Recommended products
GST Activity Assay Kit (Fluorometric) provides a simple, fluorescence-based in vitro assay for detecting GST activity using fluorescence plate reader.
Key facts
- Detection method
- Fluorescent
- Sample types
- Purified protein, Tissue Lysate, Saliva, Urine, Plasma, Serum, Other biological fluids, Cell Lysate
- Assay type
- Enzyme activity
- Assay time
- 2h
- Assay Platform
- Microplate reader
- Sensitivity
- < 0.5 mU/well
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's GST Activity Assay Kit (Fluorometric) provides a simple, fluorescence-based in vitro assay for detecting the GST activity using fluorescence plate reader. The assay utilizes monochlorobimane (MCB), a dye that reacts with glutathione. The free form of MCB is almost nonfluorescent, whereas the dye fluoresces in blue (Ex/Em = 380/461 nm) after reaction with glutathione catalysed by GST. The change in fluorescence overtime allows for an easy measurement of the sample GST level.
The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein.
Visit our FAQs page for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called K260 GST Fluorometric Activity Assay Kit. K260-100 is the same size as the 100 test size of ab65325.
Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.
- Biological function summary
GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.
- Pathways
GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.
- Associated diseases and disorders
GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.
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1 product image
Functional Studies - GST Activity Assay Kit (Fluorometric) (ab65325)
Standard Calibration Curve of GST Measured by Fluorometry. Various amounts of standard GST were incubated with GSH and MCB according to the kit instructions. Fluorescence was measured at ex/em 380/460 nm.
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Product protocols
- Visit the general protocols
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