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AB65326

GST Assay Kit (Colorimetric)

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(18 Publications)

GST Assay Kit (Colorimetric) (ab65326) is used to detect GST activity in crude cell lysates and purified protein fractions, and also to quantitate GST-tagged fusion proteins.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

Glutathione S-transferase A1, 13-hydroperoxyoctadecadienoate peroxidase, GST HA subunit 1, GST class-alpha member 1, GST-epsilon, GSTA1-1, GTH1, GSTA1

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Functional Studies - GST Assay Kit (Colorimetric) (AB65326)
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Functional Studies - GST Assay Kit (Colorimetric) (AB65326)

GST activity calculated in cell lysates, with background signal subtracted (duplicates +/- SD), using equation in protocol.

Functional Studies - GST Assay Kit (Colorimetric) (AB65326)
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Supplier Data

Functional Studies - GST Assay Kit (Colorimetric) (AB65326)

GST Kinetic Assay performed according to the protocol.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate

Assay type

Enzyme activity

Sensitivity

< 4 mU/well

Assay time

1h 30m

Assay Platform

Microplate reader

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Product details

GST Assay Kit (Colorimetric) (ab65326) is used to detect GST activity in crude cell lysates and purified protein fractions, and also to quantitate GST-tagged fusion proteins.

The GST assay protocol is based on the GST-catalyzed reaction between GSH and the GST substrate CDNB.

CDNB has the broadest range of isozyme detectability (e.g., alpha-, mu-, pi-, and other GST isoforms). The GST-catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected by spectrophotometer at 340 nm.

One unit of GST activity is defined as the amount of enzyme producing 1 umol of GS-DNB conjugate/min under the conditions of the assay. Detection limit of assay: active GST < 1 mU.

GST assay protocol summary:
- add samples and positive control to wells
- add glutathione to wells
- add reaction mix
- analyze with a microplate reader for at least 10 min

Other Notes
This product was previously called K263 Biovision GST Colorimetric Activity Assay Kit. Biovision was acquired by Abcam in 2021.

Glutathione S-transferase (GST, EC 2.5.1.13) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.
Biological function summary

GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.

Pathways

GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.

GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.
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Product protocols

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Target data

Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis (PubMed : 11152686). Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid (PubMed : 16624487).
See full target information Glutathione S-transferase A1
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:15388 PubMed38965296

2024

Proteomic analysis of Rana sylvatica reveals differentially expressed proteins in liver in response to anoxia, dehydration or freezing stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yingxi Li,Zoran Minic,Nico Hüttmann,Abdullah Khraibah,Kenneth B Storey,Maxim V Berezovski

Toxicological sciences : an official journal of the Society of Toxicology 201:73-84 PubMed38897649

2024

Altered oxidative stress and antioxidant biomarkers concentrations in pregnant individuals exposed to oil and gas sites in Northeastern British Columbia.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew W Day,Coreen Daley,Yifan Wu,Maduomethaa Pathmaraj,Marc-André Verner,Élyse Caron-Beaudoin

Scientific reports 13:14652 PubMed37670115

2023

Immuno-physiological effects of dietary reishi mushroom powder as a source of beta-glucan on Rohu, Labeo rohita challenged with Aeromonas veronii.

Applications

Unspecified application

Species

Unspecified reactive species

Tutul Kumar Saha, Mariom,Tanvir Rahman,Mohammad Moniruzzaman,Taesun Min,Zakir Hossain

Pharmaceutics 15: PubMed37111775

2023

Extracellular Vesicles and Their Mimetics: A Comparative Study of Their Pharmacological Activities and Immunogenicity Profiles.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Heng Chng,Ram Pravin Kumar Muthuramalingam,Charles Kang Liang Lou,Silas New,Yub Raj Neupane,Choon Keong Lee,Ayca Altay Benetti,Chenyuan Huang,Praveen Thoniyot,Wei Seong Toh,Jiong-Wei Wang,Giorgia Pastorin

Cells 12: PubMed36899865

2023

Differential Effects of Nonsteroidal Anti-Inflammatory Drugs in an In Vitro Model of Human Leaky Gut.

Applications

Unspecified application

Species

Unspecified reactive species

Michele d'Angelo,Laura Brandolini,Mariano Catanesi,Vanessa Castelli,Cristina Giorgio,Margherita Alfonsetti,Mara Tomassetti,Mara Zippoli,Elisabetta Benedetti,Maria Candida Cesta,Sandro Colagioia,Pasquale Cocchiaro,Annamaria Cimini,Marcello Allegretti

Metabolites 13: PubMed36677055

2023

Quercetin, a Plant Flavonol Attenuates Diabetic Complications, Renal Tissue Damage, Renal Oxidative Stress and Inflammation in Streptozotocin-Induced Diabetic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Arshad Husain Rahmani,Mohammed A Alsahli,Amjad Ali Khan,Saleh A Almatroodi

Brazilian journal of biology = Revista brasleira de biologia 84:e258234 PubMed35830129

2022

Ameliorative potential role of Rosmarinus officinalis extract on toxicity induced by etoposide in male albino rats.

Applications

Unspecified application

Species

Unspecified reactive species

Reham M Morsi,Doaa S Mansour,Amr M Mousa

Antioxidants (Basel, Switzerland) 10: PubMed34829553

2021

17β-Estradiol Abrogates Oxidative Stress and Neuroinflammation after Cortical Stab Wound Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Kamran Saeed,Myeung Hoon Jo,Jun Sung Park,Sayed Ibrar Alam,Ibrahim Khan,Riaz Ahmad,Amjad Khan,Rahat Ullah,Myeong Ok Kim

Pharmaceutics 13: PubMed33670981

2021

6-Gingerol, a Bioactive Compound of Ginger Attenuates Renal Damage in Streptozotocin-Induced Diabetic Rats by Regulating the Oxidative Stress and Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Saleh A Almatroodi,Abdullah M Alnuqaydan,Ali Yousif Babiker,Mashael Abdullah Almogbel,Amjad Ali Khan,Arshad Husain Rahmani

Antioxidants (Basel, Switzerland) 9: PubMed33053761

2020

Protective Effect of Geraniol on Oxidative, Inflammatory and Apoptotic Alterations in Isoproterenol-Induced Cardiotoxicity: Role of the Keap1/Nrf2/HO-1 and PI3K/Akt/mTOR Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Nancy S Younis,Mohamed S Abduldaium,Maged E Mohamed
View all publications
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