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AB270553

GTPase Assay Kit

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(2 Publications)

GTPase Assay Kit (ab270553) employs a 96-well plate format with all of the reagents necessary for measuring GTPase activity.
2 Images
Functional Studies - GTPase Assay Kit (AB270553)
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Supplier Data

Functional Studies - GTPase Assay Kit (AB270553)

Dynamic Range for GTPase Assay Kit.

PiColorLockTM has been designed to have a large linear range, thus reducing the need for sample dilution. Competitors' products are linear over a much narrower range of concentrations.

Functional Studies - GTPase Assay Kit (AB270553)
  • FuncS

Supplier Data

Functional Studies - GTPase Assay Kit (AB270553)

Data provided for demonstration purposes only.

Standard Curve for GTPase Assay Kit (ab270553).

Key facts

Detection method

Colorimetric

Assay type

Enzyme activity (quantitative)

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

GTPase Assay Kit (ab270553) employs a 96-well plate format with all of the reagents necessary for measuring GTPase activity. The kit also contains PiColorLock™ (a superior malachite green reagent) which has special additives to prevent backgrounds arising out of non-enzymatic GTP hydrolysis. The PiColorLock™ assay is based on the change in absorbance of the dye malachite green in the presence of phosphomolybdate complexes. Unlike most malachite dye-based solutions, PiColorLockTM gives a stable end- point signal and is not prone to precipitation. PiColorLockTM has special additives to prevent backgrounds arising from nonenzymatic GTP hydrolysis.

This product is manufactured by Expedeon, an Abcam company. It was previously called GTPase Enzyme Assay Kit. Expedeon product code 602-0120 is the same as the 2 x 96 tests size of this product.

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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab270553-2x96Tests", "assetComponentDetails": [ { "size":"1 x 500 µL", "name":"Accelerator", "number":"AB270553-CMP01", "productcode":"" }, { "size":"2 x 1 Unit", "name":"96-well clear low binding plates", "number":"AB270553-CMP05", "productcode":"" }, { "size":"1 x 5 mL", "name":"0.5 M Tris pH 7.5", "number":"AB270553-CMP08", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stabilizer", "number":"AB270553-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"0.1 mM Pi Standard", "number":"AB270553-CMP04", "productcode":"" }, { "size":"4 x 1 Vial", "name":"GTP (lyophilized)", "number":"AB270553-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"PiColorLock", "number":"AB270553-CMP06", "productcode":"" }, { "size":"1 x 1500 µL", "name":"0.1 M Magnesium chloride", "number":"AB270553-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

See full target information GTPase
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 61:5047-5070 PubMed38159198

2024

Presenilin2 D439A Mutation Induces Dysfunction of Mitochondrial Fusion/Fission Dynamics and Abnormal Regulation of GTPase Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Chenhao Gao,Junkui Shang,Zhengyu Sun,Mingrong Xia,Dandan Gao,Ruihua Sun,Wei Li,Fengyu Wang,Jiewen Zhang

Cell 184:3643-3659.e23 PubMed34166613

2021

Structural basis for VIPP1 oligomerization and maintenance of thylakoid membrane integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Tilak Kumar Gupta,Sven Klumpe,Karin Gries,Steffen Heinz,Wojciech Wietrzynski,Norikazu Ohnishi,Justus Niemeyer,Benjamin Spaniol,Miroslava Schaffer,Anna Rast,Matthias Ostermeier,Mike Strauss,Jürgen M Plitzko,Wolfgang Baumeister,Till Rudack,Wataru Sakamoto,Jörg Nickelsen,Jan M Schuller,Michael Schroda,Benjamin D Engel
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com