Skip to main content

H&E Staining Kit (Hematoxylin and Eosin) ab245880 is intended for use in histology (fixed paraffin embedded or frozen tissue section) and cytology applications.

- Uses improved Eosin formulation: lower evaporation rate, better color patterns, and improved surface tension to remain on tissue section
- Hematoxylin solution provides optimal contrast to Eosin stained cytoplasm
- Cited in over 220 publications


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (AB245880), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (AB245880), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (AB245880), expandable thumbnail

Publications

Key facts

Sample types
Tissue sections

Associated Products

Select an associated product type

2 products for Alternative Product

Additional Targets

Dtxcid201473824

What's included?

1 Kit
Components
Bluing Reagent
1 x 500 mL
Eosin Y Solution
1 x 500 mL
Hematoxylin (Modified Mayer's Solution)
1 x 500 mL

Recommended products

H&E Staining Kit (Hematoxylin and Eosin) ab245880 is intended for use in histology (fixed paraffin embedded or frozen tissue section) and cytology applications.

- Uses improved Eosin formulation: lower evaporation rate, better color patterns, and improved surface tension to remain on tissue section
- Hematoxylin solution provides optimal contrast to Eosin stained cytoplasm
- Cited in over 220 publications

Key facts

Sample types
Tissue sections

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
Storage information
Ambient

Notes

How the assay works

Included in H&E Staining Kit (Hematoxylin and Eosin) is a newly formulated Eosin that provides the benefits of a traditional alcoholic formula with significant improvements in usability. Advantages include lower evaporation rate, better color patterns, reduced tendency to spill over container, hands, and countertops, and improved surface tension to remain on tissue section. The Hematoxylin produces crisp, intense blue nuclei providing optimal contrast to the Eosin stained cytoplasm.

Cytoplasm: Light Pink
Collagen: Pink
Muscle: Pink/Rose
Erythrocytes: Pink/Red
Nuclei: Blue

Control Tissue: Any well fixed paraffin embedded or frozen tissue section. Cell smear.

H&E Staining Kit (Hematoxylin and Eosin) protocol summary

Deparaffinize and hydrate.
Apply adequate Hematoxylin and incubate for 5 minutes.
Apply adequate Bluing Reagent and incubate for 10-15 seconds.
Apply adequate Eosin Y Solution (Modified Alcoholic) and incubate for 2-3 minutes.
Clear and mount.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (ab245880), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (ab245880)

    ab245880 H&E Staining Kit used to perform Hematoxylin and Eosin staining of formalin-fixed-paraffin embedded tissue sections.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (ab245880), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (ab245880)

    ab245880 H&E Staining Kit used to perform Hematoxylin and Eosin staining of formalin-fixed-paraffin embedded tissue sections.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (ab245880), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (ab245880)

    ab245880 H&E Staining Kit used to perform Hematoxylin and Eosin staining of formalin-fixed-paraffin embedded tissue sections.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com