AB245880

H&E Staining Kit (Hematoxylin and Eosin)

Name: H&E Staining Kit (Hematoxylin and Eosin) | Abcam
Brand: Abcam Limited
SKU: AB245880
Price: 75 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(362 Publications)

H&E Staining Kit (Hematoxylin and Eosin) ab245880 is intended for use in histology (fixed paraffin embedded or frozen tissue section) and cytology applications.

- Uses improved Eosin formulation: lower evaporation rate, better color patterns, and improved surface tension to remain on tissue section
- Hematoxylin solution provides optimal contrast to Eosin stained cytoplasm
- Cited in over 220 publications

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (AB245880)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (AB245880)

ab245880 H&E Staining Kit used to perform Hematoxylin and Eosin staining of formalin-fixed-paraffin embedded tissue sections.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (AB245880)

ab245880 H&E Staining Kit used to perform Hematoxylin and Eosin staining of formalin-fixed-paraffin embedded tissue sections.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (AB245880)

ab245880 H&E Staining Kit used to perform Hematoxylin and Eosin staining of formalin-fixed-paraffin embedded tissue sections.

Key facts

Sample types

Tissue sections

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Product details

How the assay works

Included in H&E Staining Kit (Hematoxylin and Eosin) is a newly formulated Eosin that provides the benefits of a traditional alcoholic formula with significant improvements in usability. Advantages include lower evaporation rate, better color patterns, reduced tendency to spill over container, hands, and countertops, and improved surface tension to remain on tissue section. The Hematoxylin produces crisp, intense blue nuclei providing optimal contrast to the Eosin stained cytoplasm.

Cytoplasm: Light Pink
Collagen: Pink
Muscle: Pink/Rose
Erythrocytes: Pink/Red
Nuclei: Blue

Control Tissue: Any well fixed paraffin embedded or frozen tissue section. Cell smear.

H&E Staining Kit (Hematoxylin and Eosin) protocol summary

Deparaffinize and hydrate.
Apply adequate Hematoxylin and incubate for 5 minutes.
Apply adequate Bluing Reagent and incubate for 10-15 seconds.
Apply adequate Eosin Y Solution (Modified Alcoholic) and incubate for 2-3 minutes.
Clear and mount.

Other Notes

Please note, the 500 mL selling size, was previously described as 1 Kit. There has been no change to the physical product.

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What's included?

{ "values": { "500mL": { "sellingSize": "500 mL", "publicAssetCode":"ab245880-500mL", "assetComponentDetails": [ { "size":"1 x 500 mL", "name":"Bluing Reagent", "number":"AB245880-CMP01" }, { "size":"1 x 500 mL", "name":"Eosin Y Solution", "number":"AB245880-CMP03" }, { "size":"1 x 500 mL", "name":"Hematoxylin (Modified Mayer's Solution)", "number":"AB245880-CMP02" } ] }, "30mL": { "sellingSize": "30 mL", "publicAssetCode":"ab245880-30mL", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Eosin Y Solution", "number":"AB245880-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"Bluing Reagent", "number":"AB245880-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"Hematoxylin (Modified Mayer's Solution)", "number":"AB245880-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

Storage information

Ambient

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Product protocols

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Target data

See full target information Haematoxylin

Additional targets

Dtxcid201473824

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Publications (362)

Recent publications for all applications. Explore the full list and refine your search

Journal of materials science. Materials in medicine 36:56 PubMed40560284

2025

Osteogenic potential of nanostructured Cu/W/Co in Fe-Mn alloys designed for maxillofacial applications: in vivo study in dog model.

Applications

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Mohamed A Abdel Hamid,Samir A Elborolosy,Sara El Moshy,Hany R Ammar,S Sivasankaran,Walid S Salem,Elham A Hassan

The Journal of clinical investigation 135: PubMed40519164

2025

Hemodynamic forces prevent myxomatous valve disease in mice through KLF2/4 signaling.

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Jesse A Pace,Lauren M Goddard,Courtney C Hong,Liqing Wang,Jisheng Yang,Mei Chen,Yitian Xu,Martin H Dominguez,Siqi Gao,Xiaowen Chen,Patricia Mericko-Ishizuka,Can Tan,Tsutomu Kume,Wenbao Yu,Kai Tan,Wayne W Hancock,Giovanni Ferrari,Mark L Kahn

Nature communications 16:5216 PubMed40473627

2025

Control of epithelial tissue organization by mRNA localization.

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Devon E Mason,Thomas D Madsen,Alexander N Gasparski,Dong Kong,Neal Jiwnani,Terry Lechler,Jadranka Loncarek,Roberto Weigert,Ramiro Iglesias-Bartolome,Stavroula Mili

Innovation (Cambridge (Mass.)) 6:100767 PubMed40470326

2025

A high-definition spatiotemporal transcriptomic atlas of mammalian kidney development.

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Shan Jiang,Hao Yu,Ting Zhao,Yao Lu,Xuesong Li,Lei Gao,Jie Mi,Wenzhe Xia,Lanjun Geng,Panpan Li,Mingyue Chen,Mengyao Kang,Jiang Liu,Yuwen Ke

Frontiers in immunology 16:1557535 PubMed40416961

2025

Virus-specific host responses and gene signatures following infection with major SARS-CoV-2 variants of concern: role of ZBP1 in viral clearance and lung inflammation.

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Unspecified application

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Amany Elsharkawy,Hamid Reza Jahantigh,Anchala Guglani,Shannon Stone,Komal Arora,Mukesh Kumar

Scientific reports 15:18022 PubMed40410198

2025

Heterozygous interferon signaling deficient mice as animal models for Chikungunya virus infection in the heart.

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Unspecified application

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Unspecified reactive species

Shazeed-Ul Karim,Farzana Nazneen,Prince M D Denyoh,David S Bai,Damian G Romero,Fengwei Bai

Communications biology 8:736 PubMed40355528

2025

Selective serotonin reuptake inhibitors induce cardiac toxicity through dysfunction of mitochondria and sarcomeres.

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Unspecified application

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Yawei Shen,Cameron E Brown,Xiao Li,Peng Zhang,Stacey R McGee,Søren C Spina,J Ricardo Loret de Mola,Joanna L Fiddler,Haodi Wu,Qing Liu

European journal of immunology 55:e202451595 PubMed40346757

2025

Itaconate Has Limited Protective Effects in Experimental Malaria Models.

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Unspecified application

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Unspecified reactive species

Fran Prenen,Emilie Pollenus,Hanne Meers,Sofie Knoops,Rebecca Sadler,Margot Deckers,Evanna L Mills,Philippe E Van den Steen

Biology direct 20:60 PubMed40346625

2025

Prmt1-mediated methylation regulates Ncoa4 stability to transactivate Adamts genes and promote bone extracellular matrix degradation in chronic hematogenous osteomyelitis.

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Unspecified application

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Unspecified reactive species

Xun Chen,Ning Duan,Wentao Zhang,Tao Song,Fei Cong

Nature communications 16:4326 PubMed40346042

2025

Maternal gut microbiota influences immune activation at the maternal-fetal interface affecting pregnancy outcome.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Giugliano,Andrea Gatti,Martina Rusin,Tilo Schorn,Silvia Pimazzoni,Michela Calanni-Pileri,Valentina Fraccascia,Sara Carloni,Maria Rescigno

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

H&E Staining Kit (Hematoxylin and Eosin)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (AB245880)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (AB245880)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (AB245880)

Key facts

Sample types

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Publications (362)

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