H&E Staining Kit (Hematoxylin and Eosin) ab245880 is intended for use in histology (fixed paraffin embedded or frozen tissue section) and cytology applications.
- Uses improved Eosin formulation: lower evaporation rate, better color patterns, and improved surface tension to remain on tissue section
- Hematoxylin solution provides optimal contrast to Eosin stained cytoplasm
- Cited in over 220 publications
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- The journal of gene medicine 26:e36882024MEG3 shuttled by exosomes released from human bone marrow mesenchymal stem cells promotes TP53 stability to regulate MCM5 transcription in keloid fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeibin Zhu,Yuanjian Ye,Ying Shao,Chunli XuePubMed 38686583
- Cell death & disease 15:2462024Genetic and pharmacological reduction of CDK14 mitigates synucleinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJean-Louis A Parmasad,Konrad M Ricke,Benjamin Nguyen,Morgan G Stykel,Brodie Buchner-Duby,Amanda Bruce,Haley M Geertsma,Eric Lian,Nathalie A Lengacher,Steve M Callaghan,Alvin Joselin,Julianna J Tomlinson,Michael G Schlossmacher,William L Stanford,Jiyan Ma,Patrik Brundin,Scott D Ryan,Maxime W C RousseauxPubMed 38575601
- Heliyon 10:e235612024Restoring glucose balance: Conditional HMGB1 knockdown mitigates hyperglycemia in a Streptozotocin induced mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZeyu Liu,Gowtham Annarapu,Hamza O Yazdani,Qinge Wang,Silvia Liu,Jian-Hua Luo,Yan-Ping Yu,Baoguo Ren,Matthew D Neal,Satdarshan P Monga,Roberto Ivan Mota AlvidrezPubMed 38187339
- Asian Pacific journal of cancer prevention : APJCP 24:4321-43272024Diagnostic Accuracy of Immunohistochemistry for HER2-Positive Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiwaporn Thanasan,Kweekrong Sukhakul,Sakchai Chitpakdee,Nakarin KitkumthornPubMed 38156869
- Frontiers in oncology 13:12399572024BME-free primary patient-specific organoids obtained with a one-day mimicking method to replicate the corresponding tumor for personalized treatment options.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Zhu,Zhechun Ding,Yini Wang,Qing Wu,Dongmei Chen,Luanhong Wang,Yuancheng Li,Yao Yao,Jiman Huang,Yun Li,Xiaojing Wang,Yanchun Lin,Tian Guan,Haoyu Zeng,Congzhu LiPubMed 38162496
- Advanced healthcare materials 13:e23033372023Nanointegrative Glycoengineering-Activated Necroptosis of Triple Negative Breast Cancer Stem Cells Enables Self-Amplifiable Immunotherapy for Systemic Tumor Rejection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoubo Zhao,Yanan Li,Jing He,Menghuan Li,Xuemei Yao,Huocheng Yang,Zhong Luo,Peng Luo,Min SuPubMed 38154036
- Cells 12:2023Daphnetin Alleviates Bleomycin-Induced Pulmonary Fibrosis through Inhibition of Epithelial-to-Mesenchymal Transition and IL-17A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSoo-Jin Park,Hyung Won Ryu,Ji-Hyeong Kim,Hwa-Jeong Hahn,Hyun-Jae Jang,Sung-Kyun Ko,Sei-Ryang Oh,Hyun-Jun LeePubMed 38132116
- International journal of molecular medicine 53:2023An LGR4 agonist activates the GSK‑3β pathway to inhibit RANK‑RANKL signaling during osteoclastogenesis in bone marrow‑derived macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuria Jang,Hyeonjoon Lee,Yongjin Cho,Eunseo Choi,Suenghwan Jo,Hong Moon Sohn,Beom Chang Kim,Young Jong Ko,Wonbong LimPubMed 38063193
- International journal of molecular sciences 24:2023Gintonin Alleviates HCl/Ethanol- and Indomethacin-Induced Gastric Ulcers in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHan-Sung Cho,Tae Woo Kwon,Ji-Hun Kim,Rami Lee,Chun-Sik Bae,Hyoung-Chun Kim,Jong-Hoon Kim,Sun-Hye Choi,Ik-Hyun Cho,Seung-Yeol NahPubMed 38069044
- Turkish archives of otorhinolaryngology 61:124-1332023Effects of Cross-linked Hyaluronic Acid in a Rat Model of Vestibular and Cochlear Toxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErdal Erkoç,Aslı Çakır Çetin,Serpil Mungan Durankaya,Serap Çilaker Mıçılı,Pembe Keskinoğlu,Osman Yılmaz,Günay Kırkım,Enis Alpin GüneriPubMed 38020411
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- Sample types
- Tissue sections
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Dtxcid201473824
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H&E Staining Kit (Hematoxylin and Eosin) ab245880 is intended for use in histology (fixed paraffin embedded or frozen tissue section) and cytology applications.
- Uses improved Eosin formulation: lower evaporation rate, better color patterns, and improved surface tension to remain on tissue section
- Hematoxylin solution provides optimal contrast to Eosin stained cytoplasm
- Cited in over 220 publications
Key facts
- Sample types
- Tissue sections
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
- Storage information
- Ambient
Notes
Included in H&E Staining Kit (Hematoxylin and Eosin) is a newly formulated Eosin that provides the benefits of a traditional alcoholic formula with significant improvements in usability. Advantages include lower evaporation rate, better color patterns, reduced tendency to spill over container, hands, and countertops, and improved surface tension to remain on tissue section. The Hematoxylin produces crisp, intense blue nuclei providing optimal contrast to the Eosin stained cytoplasm.
Cytoplasm: Light Pink
Collagen: Pink
Muscle: Pink/Rose
Erythrocytes: Pink/Red
Nuclei: Blue
Control Tissue: Any well fixed paraffin embedded or frozen tissue section. Cell smear.
H&E Staining Kit (Hematoxylin and Eosin) protocol summary
Deparaffinize and hydrate.
Apply adequate Hematoxylin and incubate for 5 minutes.
Apply adequate Bluing Reagent and incubate for 10-15 seconds.
Apply adequate Eosin Y Solution (Modified Alcoholic) and incubate for 2-3 minutes.
Clear and mount.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (ab245880)
ab245880 H&E Staining Kit used to perform Hematoxylin and Eosin staining of formalin-fixed-paraffin embedded tissue sections.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (ab245880)
ab245880 H&E Staining Kit used to perform Hematoxylin and Eosin staining of formalin-fixed-paraffin embedded tissue sections.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - H&E Staining Kit (Hematoxylin and Eosin) (ab245880)
ab245880 H&E Staining Kit used to perform Hematoxylin and Eosin staining of formalin-fixed-paraffin embedded tissue sections.
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